en Pharmacology Pharmacology مقاله پژوهشی Original Research Article <p style="text-align: justify;"><span style="font-size:16px;"><span style="font-family:Times New Roman;"><b><span style="background:#2d7f8f"><span style="color:white">Background & Objective:</span></span><b> </b>&nbsp;</b><span style="color:black">The incidence of asthma is rising, incurring considerable healthcare expenses. Although corticosteroids are the most effective anti-inflammatory drugs for improving lung function in asthma, they have a limited influence on airway remodeling and can cause adverse effects. Therefore, interest in developing natural medications with fewer side effects is growing. The increasing use of traditional herbal medicine demands more scientific evidence for its efficacy. <i>Allium ampeloprasum</i>, which is suggested for use in traditional medicine to treat asthma, was chosen for this investigation due to its traditional applications.</span><br> <b><span style="background:#2d7f8f"><span style="color:white"><span style="letter-spacing:-.1pt">&nbsp;Materials & Methods:</span></span></span></b> &nbsp;<span lang=""><span style="color:black"><span style="letter-spacing:-.1pt">A hydroalcoholic extract of the plant was prepared. Its effect on the viability and proliferation of airway smooth muscle cells was determined using the MTT assay. The production of nitric oxide and the levels of inflammatory proteins in T lymphocyte cells were also investigated.</span></span></span><span style="color:black"><span style="letter-spacing:-.1pt"></span></span><br> <b><span lang="EN-IN"><span style="background:#2d7f8f"><span style="color:white">Results: </span></span></span>&nbsp;</b><i><span style="color:black"><span style="letter-spacing:-.1pt">A. ampeloprasum </span></span></i><span style="color:black"><span style="letter-spacing:-.1pt">extract significantly reduced the proliferation of airway smooth muscle cells in a dose- and time-dependent manner (p &le; 0.05). Platelet-derived growth factor-BB (PDGF-BB) enhanced proliferation, whereas the extract significantly suppressed this PDGF-BB-induced proliferation (p &le; 0.05). The extract did not influence basal cell viability or proliferation, regardless of LPS stimulation. In LPS-stimulated cells, the extract significantly decreased nitric oxide production (p &le; 0.05). The expression and production of LPS-induced inflammatory markers were also significantly reduced (p &le; 0.05).</span></span><br> <b><span style="background:#2d7f8f"><span style="color:white">Conclusion: </span></span>&nbsp;</b><i><span lang=""><span style="color:black">A. ampeloprasum</span></span></i><span lang=""><span style="color:black"> has anti-inflammatory effects and can prevent the proliferation of airway smooth muscle cells. </span></span><span style="color:black">It can be proposed as a possible corticosteroid substitute.</span></span></span><br> <p></p></p> Asthma, Inflammation, A. Ampeloprasum, T Lymphocytes 327 336 http://journal.zums.ac.ir/browse.php?a_code=A-10-7224-1&slc_lang=en&sid=1 Shalaw Haji Ahmed shalawhaji23@gmail.com 5200319475328460087466 0009-0000-1976-0788 No Student Research Committee, Kermanshah University of Medical Sciences, Kermanshah, Iran Mohammed Namiq Amin mnamiq771@gmail.com 5200319475328460087467 5200319475328460087467 No Department of Anatomical Sciences, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran Ali Ghanbari aligharak@yahoo.com 5200319475328460087468 5200319475328460087468 No Department of Anatomical Sciences, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran Mona Pazhouhi mona.pazhouhi@gmail.com 5200319475328460087469 5200319475328460087469 No Student Research Committee, Kermanshah University of Medical Sciences, Kermanshah, Iran Iraj Rashidi iraj.rashidi@kums.ac.ir 5200319475328460087470 5200319475328460087470 Yes Department of Anatomical Sciences, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran