Journal of Advances in Medical and Biomedical Research

en IL-1B Gene Polymorphisms and Their Association with Chronic Gastritis and Gastric Cancer in Helicobacter pylori Positive Patients in Northwestern Iran Medical Biology Medical Biology مقاله پژوهشی Original Research Article Background & Objective: Interactions between Helicobacter pylori (H. pylori) and the human host particularly gastric inflammation and cytokine-mediated signaling have been extensively investigated due to their critical role in the pathogenesis of gastric disorders. This study aimed to evaluate the association between IL1B gene polymorphisms and the risk of chronic gastritis and gastric cancer in H. pylori–infected individuals from Northwestern Iran, a region with a considerable burden of gastric disease.  Materials & Methods: A total of 210 participants were enrolled and stratified into three groups: healthy controls, patients with chronic gastritis, and patients with gastric cancer (70 individuals per group). Peripheral blood samples collected in EDTA tubes were used for serological detection of H. pylori using an ELISA assay (Pishtaz Teb diagnostic kit) and for genomic DNA extraction. Genotyping of IL1B polymorphisms at positions −31, −511, and +3954 was performed using polymerase chain reaction–restriction fragment length polymorphism (PCR-RFLP) analysis. Results:  Of the 210 participants, only seven individuals in the gastritis group were positive for H. pylori. The CT genotype of IL1B −31 was more frequently observed in H. pylori–negative patients with gastritis. At the IL1B −511 locus, the CC genotype was significantly more prevalent among H. pylori negative healthy controls, suggesting a protective effect against gastritis (p = 0.023). Additionally, the combined CT+TT genotypes at this locus were significantly associated with gastritis in H. pylori positive individuals (p = 0.010). No significant associations were detected between the IL1B +3954 polymorphism and disease status or H. pylori infection. Conclusion:  These findings demonstrate a significant association between IL1B gene polymorphisms and susceptibility to chronic gastritis and gastric cancer, particularly in the context of H. pylori infection. Further large-scale studies across diverse populations are warranted to elucidate the contribution of host genetic factors to gastric disease risk. IL-1β, Polymorphism, Gastritis, Gastric Cancer, H. pylori 41 50 http://journal.zums.ac.ir/browse.php?a_code=A-10-7257-1&slc_lang=en&sid=1 Ali Sadighi ali.sadighi1996@gmail.com 5200319475328460088598 5200319475328460088598 No Liver and Gastrointestinal Disease Research Center, Tabriz University of Medical Sciences, Tabriz, Iran Amir Mehdizadeh mehdizadeha@outlook.com 5200319475328460088599 5200319475328460088599 No Hematology and Oncology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran Mohammad Hossein Somi Mhosseinsina@yahoo.com 5200319475328460088600 5200319475328460088600 No Liver and Gastrointestinal Disease Research Center, Tabriz University of Medical Sciences, Tabriz, Iran Sousan Mirnajd Gerami Smirnajd@gmail.com 5200319475328460088601 5200319475328460088601 No Liver and Gastrointestinal Disease Research Center, Tabriz University of Medical Sciences, Tabriz, Iran Leila Rahbarnia Le.rahbarnia@gmail.com 5200319475328460088602 5200319475328460088602 No Infectious and Tropical Disease Research Center, Tabriz University of Medical Sciences, Tabriz, Iran Mahsa Esmaeillou M_esmaeillu92@ms.tabrizu.ac.ir 5200319475328460088603 5200319475328460088603 No Microbiologist, Latis Scientific, London, UK Suna Kızılyıldırım sunakizilyildirim@sdu.edu.tr 5200319475328460088604 5200319475328460088604 No Department of Pharmaceutical Microbiology, Faculty of Pharmacy, Süleyman Demirel University, Isparta, Turkey Ali Bahadori Bahadoria@tbzmed.ac.ir 5200319475328460088605 5200319475328460088605 Yes Liver and Gastrointestinal Disease Research Center, Tabriz University of Medical Sciences, Tabriz, Iran