Journal of Advances in Medical and Biomedical Research

en Co-culture-based three-dimensional culture platforms to enhance the in vitro survival and proliferation of U266 myeloma cells Medical Biology Medical Biology مقاله پژوهشی Original Research Article Background & Objective:  It has been demonstrated that three-dimensional (3D) interactions among myeloma cells, other cellular components, and extracellular matrix (ECM) constitutes within the bone marrow (BM) microenvironment play critical roles in disease progression. Therefore, the in vitro recapitulation of interactions would advance the fields of disease modeling and drug screening. In this regard, we aimed to develop simple, cost-effective, and available 3D culture conditions for U266 cells using co-culture systems to more closely mimic their native microenvironment.  Materials & Methods:  U266 cells, BM-derived mesenchymal stem cells (BM-MSCs) and human umbilical vein endothelial cells (HUVECs) were co-cultured in different matrix-based and matrix-free conditions. Subsequently, the viability and proliferation rates of U266 cells were evaluated and compared. Peripheral blood (PB) plasma was used to generate fibrin gels for matrix-based structures. Results:  Co-cultured cells in the gel-free group generated and assembled into 3D cell structures. Moreover, the viability and expansion fold of U266 cells in the gel-free group were significantly higher compared with those in the on-gel and inside-gel groups. Conclusion:  Our simple gel-free 3D co-culture system of U266 cells with BM-MSCs and HUVECs, which demonstrated higher viability and proliferation rates of myeloma cells, can serve as a platform for further in vitro studies, including drug screening. multiple myeloma, co-culture, three-dimensional cell culture, fibrin, proliferation 251 260 http://journal.zums.ac.ir/browse.php?a_code=A-10-7370-1&slc_lang=en&sid=1 Mohammadmahdi Khosravi mohammadmahdy2012@gmail.com 5200319475328460090746 0009-0003-8281-0014 No Department of Hematology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran Sadaf Vahdat s_vahdat@modares.ac.ir 5200319475328460090747 5200319475328460090747 Yes Applied Cell Sciences Division, Department of Hematology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran Saeid Abroun abroun@modares.ac.ir 5200319475328460090748 0009-0005-0242-5621 No Department of Hematology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran