en Life Science Life Science مقاله پژوهشی Original Research Article <p style="text-align: justify;"><span style="font-size:16px;"><span style="font-family:Times New Roman;"><b><span style="background:#2d7f8f"><span style="color:white">Background & Objective:</span></span><b> </b>&nbsp;</b><span lang="EN"><span style="color:black">Non-small cell lung cancer (NSCLC) stands as the predominant&nbsp;subtype of lung malignancy globally, which persists as a major driver of cancer-related deaths. This study systematically investigates bioinformatics-based investigations aimed at identifying differentially expressed genes (DEGs) and potential therapeutic targets associated with NSCLC.</span></span><b><i><span lang="EN"><span style="color:black"></span></span></i></b><br> <b>&nbsp;<span lang="EN-IN"><span style="background:#2d7f8f"><span style="color:white"><span style="letter-spacing:-.1pt">Materials & Methods:</span></span></span></span></b> &nbsp;<span lang="EN"><span style="color:black"><span style="letter-spacing:-.1pt">Publicly available RNA expression datasets were analyzed using advanced in silico tools, including the enrichr function from the GSEApy package and R statistical software (v4.3.1), to uncover molecular pathways involved in disease progression. Key oncogenic signaling pathways such as <i>TGF-&beta; </i>signaling, Wnt/&beta;-catenin, PI3K-AKT-mTOR, and MAPK were found to be </span></span></span><span lang="EN"><span style="color:black"><span style="letter-spacing:-.1pt">significantly associated with several genes, such as <i>AGER, ANK3, CSTA, FGG, AGR2, BRCA1, HDAC1, miR-577, TRIM29, PIK3CA,</i> and <i>JNK</i>. Pathway enrichment analysis further identified significant involvement in chronic myeloid leukemia, longevity regulation, thyroid hormone signaling, viral carcinogenesis, Epstein-Barr virus infection, and microRNAs in cancer.</span></span></span><span style="color:black"><span style="letter-spacing:-.1pt"></span></span><br> <b><span lang="EN-IN"><span style="background:#2d7f8f"><span style="color:white">Results: </span></span></span>&nbsp;</b><span lang="EN"><span style="color:black"><span style="letter-spacing:-.1pt">The correlation analysis revealed that <i>TRIM29</i> expression was higher in control samples, whereas <i>AGR2</i> was prominently expressed in NSCLC samples. Additionally, <i>HDAC1</i> and <i>BRCA1</i> were identified as promising diagnostic biomarkers.</span></span></span><span style="color:black"><span style="letter-spacing:-.1pt"></span></span><br> <b><span style="background:#2d7f8f"><span style="color:white">Conclusion: </span></span>&nbsp;</b><span lang="EN"><span style="color:black">These findings improve our comprehension of NSCLC disease mechanisms while identifying promising molecular candidates for formulating more potent, customized treatment approaches,&nbsp;potentially improving clinical outcomes for patients.</span></span></span></span></p> Non-small Cell Lung Cancer (NSCLC), Bioinformatics Analysis, Differentially Expressed Genes (DEGs), Pathway Enrichment, RNA-seq, Therapeutic Targets 293 306 http://journal.zums.ac.ir/browse.php?a_code=A-10-7177-3&slc_lang=en&sid=1 Aditya Kumar Jha adityajha.bhavans@gmail.com 5200319475328460087453 5200319475328460087453 No High-School Junior, VIBGYOR High School, Mumbai, India Mohammad Ali Abdullah Almoyad Almoyadmaa@mail.com 5200319475328460087454 5200319475328460087454 No Department of Basic Medical Sciences, College of Applied Medical Sciences, King Khalid University, Abha, Saudi Arabia Shadma Wahab Wahabs@mail.com 5200319475328460087455 5200319475328460087455 No Department of Pharmacognosy, College of Pharmacy, King Khalid University, Abha, Saudi Arabia Garima Gupta garimarock2014@gmail.com 5200319475328460087456 5200319475328460087456 No Graphic Era Hill University, Dehradun, India Khang Wen Goh khangwen.goh@newinti.edu.my 5200319475328460087457 5200319475328460087457 No Faculty of Business and Communication, INTI International University, Nilai, Malaysia Amirhossein Sahebkar amir_saheb2000@yahoo.com 5200319475328460087458 5200319475328460087458 Yes Department of Biochemistry, Saveetha Medical College and Hospital, Saveetha Institute of Medical and Technical Sciences, Chennai, Tamil Nadu, India Prashant Kesharwani prashantdops@gmail.com 5200319475328460087459 5200319475328460087459 No Next-Generation Translational Nanomedicine Laboratory, Department of Pharmaceutical Sciences, Dr. Harisingh Gour Vishwavidyalaya, Sagar, Madhya Pradesh, India