Journal of Advances in Medical and Biomedical Research

en Ameliorative Role of Resveratrol in Silver Nanoparticle–Induced Oxidative Stress and Testicular Alterations in Male Mice Pharmacology Pharmacology مقاله پژوهشی Original Research Article Background & Objective:  Silver nanoparticles (AgNPs) are among the most commonly used silver derivatives, and, due to their potential reproductive toxicity, may pose a potential hazard to fertility. This study aimed to evaluate the protective effect of resveratrol against AgNPs-induced testicular damage and oxidative stress in a male mice model.  Materials & Methods:  In this experimental study, 24 adult male mice were randomly divided into four groups (n=6/group): control, AgNPs (200 mg/kg), resveratrol (20 mg/kg), and a combination of AgNPs and resveratrol. Treatments were administered daily by oral gavage for 35 days. At the end of the treatment period, serum malondialdehyde (MDA), total antioxidant capacity (TAC), superoxide dismutase (SOD), testosterone, testicular 8-OHdG levels, and histological changes including seminiferous tubule diameter, Leydig cell count, tissue structure, and spermatogenic status, were evaluated and analyzed among the groups. Results:  AgNPs significantly reduced serum testosterone levels and increased oxidative stress, as indicated by elevated SOD and 8-OHdG levels (P< 0.001). Resveratrol treatment significantly restored testosterone levels and reduced oxidative DNA damage, with values approaching those of the control group (P< 0.01). No significant changes were observed in TAC and MDA levels among the groups. AgNPs caused marked reductions in seminiferous tubule diameter, epithelial thickness, and counts of primary spermatocytes, spermatozoa, Sertoli, and Leydig cells (P<0.001). These adverse effects were significantly ameliorated by resveratrol, particularly in the resveratrol-only group (P<0.01). Conclusion:  AgNPs induce oxidative stress-mediated testicular damage and hormonal disruption in male mice. While resveratrol exerts a protective role by mitigating some of these effects, its impact was limited and did not fully reverse AgNP-induced toxicity. Silver nanoparticles, Resveratrol, Reproductive Toxicity, Testicular Damage, Mice.Mice. 274 280 http://journal.zums.ac.ir/browse.php?a_code=A-10-4479-2&slc_lang=en&sid=1 Mohammad amin behmanesh behmanesh.ma@yahoo.com 5200319475328460090757 5200319475328460090757 No Department of Histology, School of Medicine, Dezful University of Medical Sciences, Dezful, Iran sima janati sjanati@ymail.com 5200319475328460090758 5200319475328460090758 No Department of Obstetrics and Gynecology, School of Medicine, Research and Clinical Center for Infertility, Dezful University of Medical Sciences, Dezful, Iran rezvan reyhani rezvan98reyhani@yahoo.com 5200319475328460090759 0009-0003-9321-1068 No Student research committee, Dezful University of medical science, Dezfu, Iran Seyedeh Mahsa poormoosavi m.poormosavi@ymail.com 5200319475328460090760 5200319475328460090760 Yes Department of Histology, School of Medicine, Research and Clinical Center for Infertility Dezful University of Medical Sciences, Dezful, Iran