en Pharmacology Pharmacology مقاله پژوهشی Original Research Article <p style="text-align: justify;"><span style="font-size:16px;"><span style="font-family:Times New Roman;"><b><span style="background:#2d7f8f"><span style="color:white">Background & Objective:</span></span><b> </b></b><span style="color:black">Chalcones are promising compounds in the pharmaceutical field due to their antioxidant and anticancer properties. The aim of this study was to synthesize two novel chalcone derivatives (A₁ and A₂) and evaluate their biological activities, including antioxidant potential and cytotoxicity against cancer cells.</span><span style="color:black"></span><br> <b><span style="background:#2d7f8f"><span style="color:white"><span style="letter-spacing:-.1pt">&nbsp;Materials & Methods:</span></span></span></b> <span style="color:black"><span style="letter-spacing:-.1pt">The chemical structures of compounds A₁ and A₂ were confirmed using spectroscopic techniques including Proton Nuclear Magnetic Resonance (¹H-NMR), Gas Chromatography-Mass Spectrometry (GC-MS), and Ultraviolet-Visible (UV-Vis) spectroscopy. A hemolysis assay was conducted to assess biocompatibility. Antioxidant activity was measured using the DPPH radical scavenging assay at various concentrations (12.4-1000&micro;g/ml). Cytotoxicity was evaluated against human breast cancer cells (MCF-7).</span><span style="letter-spacing:-.1pt"></span></span><br> <b><span lang="EN-IN"><span style="background:#2d7f8f"><span style="color:white">Results: </span></span></span>&nbsp;</b><span style="color:black"><span style="letter-spacing:-.1pt">Both A₁ and A₂ exhibited low hemolytic activity (4.09% and 3.99% respectively, at 100&micro;g/ml), indicating good biocompatibility. Compound A₁ exhibited more potent antioxidant activity than A₂. Cytotoxicity assays demonstrated that both compounds were more toxic to MCF-7 cancer cells, with IC₅₀ values for the produced compounds A1, A2, and Tamoxifen were 34.67&micro;g/ml, 28.34&micro;g/ml, and 15.48&micro;g/ml, respectively, indicating potential selective anticancer activity.</span><span style="letter-spacing:-.1pt"></span></span><br> <b><span style="background:#2d7f8f"><span style="color:white">Conclusion: </span></span>&nbsp;</b><span style="color:black">Compounds A₁ and A₂ exhibited promising antioxidant and anticancer properties, with minimal hemolytic effects and selective toxicity toward cancer cells, making them potential candidates for further pharmaceutical development.</span></span></span></p> Sulfonamide Chalcone Derivatives, Antioxidant, Hemolysis, In silico Docking, GC-MS, 1H-NMR, UV-Vis, MCF-7 Breast Cancer 110 122 http://journal.zums.ac.ir/browse.php?a_code=A-10-7448-1&slc_lang=en&sid=1 Zainab Y. Kadhim zaniabchemo2@mu.edu.iq 5200319475328460087617 5200319475328460087617 No Department of Physiology, College of Veterinary Medicine, Al-Muthanna University, Samawah, Iraq Sarah A. Al-khafaji sarahbiology89@mu.edu.iq 5200319475328460087618 5200319475328460087618 No Department of Microbiology, College of Veterinary Medicine, Al-Muthanna University, Samawah, Iraq Rusul N. Mankhi rusul.naaem@uomisan.edu.iq 5200319475328460087619 0009-0008-8365-913X Yes Department of Pharmaceutical Chemistry, College of Pharmacy, University of Misan, Maysan, Iraq Manar A. Abddulameer manaradnan689@gmail.com 5200319475328460087620 0009-0007-9396-0893 No Department of Sciences, College of Basic Education, Al-Muthanna University, Samawah, Iraq