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Volume 19, Issue 74 (3-2011)
J Adv Med Biomed Res 2011, 19(74): 9-19 |
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Khorsandi L, Bahramzadeh S, Hashemitabar M, Kalantar Mahdavi S R. Metformin Effect on the Mouse Pancreatic Langerhans Islets Volume. J Adv Med Biomed Res 2011; 19 (74) :9-19
URL: http://journal.zums.ac.ir/article-1-1411-en.html
URL: http://journal.zums.ac.ir/article-1-1411-en.html
Laya Khorsandi *1 
, Somayeh Bahramzadeh2 , Mahmoud Hashemitabar2 , Seyed Rashidodin Kalantar Mahdavi2
, Somayeh Bahramzadeh2 , Mahmoud Hashemitabar2 , Seyed Rashidodin Kalantar Mahdavi2
1- Cell and Molecular Research Center, Ahwaz Jundishapur University of Medical Sciences, Ahwaz, Iran , Layasadat@yahoo.com
2- Cell and Molecular Research Center, Ahwaz Jundishapur University of Medical Sciences, Ahwaz, Iran
2- Cell and Molecular Research Center, Ahwaz Jundishapur University of Medical Sciences, Ahwaz, Iran
Abstract: (171305 Views)
Background and Objective: Metformin is a widely used medicine for treatment of type 2 diabetes. In this study, the effect of various doses of metformin on the mouse islets of langerhans volume was investigated.
Materials and methods: Twenty four C57BL/6 adult male mice weighting 30±5 gr were randomly divided into 4 groups. Normal saline was given to the control group (group 4) and the experimental groups (groups 1-3) received 75, 150 and 300 mg/kg metformin daily by intraperitoneal injection for seven days. One day after the last injection the mice were sacrificed by cervical dislocation and their pancreases were fixed in 10% formalin for histological studies. The volume of the islets of langerhans was estimated by using Cavalieri method.
Results: Volume of the islets of langerhans in doses of 75 and 150 mg/kg Metformin showed a non-significant difference in comparison to control group (P>0.05). 300 mg/kg metformin treated mice showed a significant increase in islets of langerhans volume compared to the control group (P<0.05).
Conclusion: Metformin increases in the islets of langerhans volume in a dose-dependent manner. Increasing effects of Metformin on the islets of langerhans volume may be due to proliferation or hypertrophy of beta cells.
Type of Study: Original Research Article |
Received: 2011/03/8 | Accepted: 2014/06/23 | Published: 2014/06/23
Received: 2011/03/8 | Accepted: 2014/06/23 | Published: 2014/06/23
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