جمعه 6 تیر 1404
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دوره 19، شماره 77 - ( 4-1390 )
جلد 19 شماره 77 صفحات 61-53 |
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Sharifi yazdi M K, Azarsa M, Shirazi M H, Rastegar Lari A, Owlia P, fallah Mehrabadi J, et al . The Frequency of Extended Spectrum Beta Lactamase and CTX M-I of Escherichia Coli Isolated from the Urine Tract Infection of Patients by Phenotypic and PCR Methods in the City of Khoy in Iran. J Adv Med Biomed Res 2011; 19 (77) :53-61
URL: http://journal.zums.ac.ir/article-1-1657-fa.html
URL: http://journal.zums.ac.ir/article-1-1657-fa.html
شریفی یزدی محمد کاظم، آذرسا محمد، شیرازی محمدحسن، رستگاری لاری عبدالعزیز، اولیا پرویز، فلاح مهر آبادی جلیل، و همکاران. و همکاران. و همکاران.. فراوانی بتالاکتامازهای وسیع الطیف و گروه CTX-M-I در سویه‌های اشریشیا کلی جدا شده از عفونت‌های ادراری به دو روش فنوتیپی وPCR در خوی، ایران. Journal of Advances in Medical and Biomedical Research. 1390; 19 (77) :53-61
محمد کاظم شریفی یزدی 
، محمد آذرسا ، محمدحسن شیرازی ، عبدالعزیز رستگاری لاری ، پرویز اولیا ، جلیل فلاح مهر آبادی ، هدروشا ملا آقا میرزایی ، آیلار صباغی ، فرناز شامکانی ، گلناز مبصری ، روناک بختیاری ، محمدمهدی سلطان دلال*1
، محمد آذرسا ، محمدحسن شیرازی ، عبدالعزیز رستگاری لاری ، پرویز اولیا ، جلیل فلاح مهر آبادی ، هدروشا ملا آقا میرزایی ، آیلار صباغی ، فرناز شامکانی ، گلناز مبصری ، روناک بختیاری ، محمدمهدی سلطان دلال*1
1- ، soltanirad34@yahoo.com
چکیده: (174324 مشاهده)
Background and Objective: The production of Extended Spectrum Beta Lactamases (ESBLs) by Escherichia coli is the main cause of resistance to Cephalosporins. In the past decade, CTX-M enzymes have become the most prevalent ESBLs in Europe, Canada, and Asia. In this study, the frequency of ESBL-producing E.coli and molecular detection of the CTX-M-I group was investigated.
Materials and Methods: A total of 400 urine samples were collected from both hospitalized and out-patients in Khoy’s hospitals between November 2009 and April 2010. Out of these samples, 188 were identified as E.coli by standard biochemical tests. The antibiotic Susceptibility tests to 10 antibiotics were performed by the-disk-agar diffusion (DAD) method. ESBL production was screened by phenotypic test that including disk diffusion agar and combined disk as recommended by the Clinical and Laboratory Standards Institute (CLSI). Screened isolates were investigated by PCR assay for detection of CTX-M-I group genes.
Results: The results show that out of 188 E.coli isolates identified, 56 (29.8%) were producing ESBls by phenotypic test. All isolates were sensitive to imipenem. Overall, 49 (87.5%) isolates were confirmed as CTX-M-I producer by PCR.
Conclusion: The results of this study showed that about 30% of the identified E.coli were producing ESBl. Therefore, we recommend to use molecular methods in such researches.
نوع مطالعه: مقاله پژوهشی |
دریافت: 1390/8/26 | پذیرش: 1393/4/1 | انتشار: 1393/4/1
دریافت: 1390/8/26 | پذیرش: 1393/4/1 | انتشار: 1393/4/1
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