دوره 29، شماره 133 - ( 11-1399 )                   جلد 29 شماره 133 صفحات 67-63 | برگشت به فهرست نسخه ها


XML English Abstract Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Ghourchian S, Douraghi M, Baghani A, Soltan Dallal M M. Detection and Frequency of Enterotoxin (cpa,cpe) Genes of Clostridium perfringens Isolated from Dehydrated Vegetables by PCR. J Adv Med Biomed Res 2021; 29 (133) :63-67
URL: http://journal.zums.ac.ir/article-1-5955-fa.html
Detection and Frequency of Enterotoxin (cpa,cpe) Genes of Clostridium perfringens Isolated from Dehydrated Vegetables by PCR. Journal of Advances in Medical and Biomedical Research. 1399; 29 (133) :63-67

URL: http://journal.zums.ac.ir/article-1-5955-fa.html


چکیده:   (142681 مشاهده)

 Background and Objective: Clostridium perfringens is an anaerobic bacterium, commonly present in retail foods. Its enterotoxin-producing ability, short generation time, ability to grow at elevated temperatures, and spore-forming ability, allows it to survive in food-processing temperatures, and cause foodborne illness. The aim of study was to screen dehydrated vegetables contaminated with cpe and cpa carrying C.perfringens.
 Materials and Methods: This is descriptive-analytical study, was carried out on 140 samples (70 unpacked and  70 packed) dehydrated vegetables collected from different areas of Tehran. Samples were inoculated on peptone and sulfite polymyxin sulfadiazine (SPS) agar for enrichment. The enrichment culture was then incubated on anaerobic condition for 48 hours. The black colonies were selected for identification test and PCR. The bacterial colonies were identified by biochemical tests, and duplex PCR was performed for α-toxin (cpa) and enterotoxin (cpe) genes.
 Results: In general 13 samples (9.3%) were identified as C. perfringens using phenotypic methods, all of the isolates were also positive for cpa but negative for cpe gene. The contamination rate for packed vegetables was 12.8% and for unpacked was 5.7%.
 Conclusion: Our finding showed that contamination of packed dehydrated vegetables was higher than unpacked; this might be due to drying as well as packaging process. We found that these isolates were negative for enterotoxin.

متن کامل [PDF 358 kb]   (153204 دریافت)    
نوع مطالعه: مقاله پژوهشی | موضوع مقاله: Clinical medicine
دریافت: 1399/1/6 | پذیرش: 1399/4/26 | انتشار: 1399/9/14

فهرست منابع
1. Lindström M, Heikinheimo A, Lahti P, et al.Novel insights into the epidemiology of Clostridium perfringens type A food poisoning. Food microbiol.2011;28(2):192-198. [DOI:10.1016/j.fm.2010.03.020]
2. ScallanE, Hoekstra RM, Angulo FJ,et al. ( 2011). Foodborne illness acquired in the United States-major pathogens. Emerg Infect Dis,17(1).7-15. [DOI:10.3201/eid1701.P11101]
3. Grass JE, Gould LH , Mahon BE. Epidemiology of foodborne disease outbreaks caused by Clostridium perfringens, United States, 1998-2010. Foodborne PathogDis.2013;10(2):131-36. [DOI:10.1089/fpd.2012.1316]
4. Bos J, Smithee L, McClane B, et al.Fatal necrotizing colitis following a foodborne outbreak of enterotoxigenic Clostridium perfringens type A infection. Clin Infect Diseas.2005; 40(10):78-83. [DOI:10.1086/429829]
5. SchneiderK.R, Goodrich-Schneider R, Hubbard MA, Richardson S.(2017). Preventing foodborne illness associated with Clostridium perfringens. FSHN035,1-6.
6. Hassan KA, Elbourne LD, Tetu SG, et al. Genomic analyses of Clostridium perfringens isolates from five toxinotypes. Res Microbiol.2015;166(4):255-263. [DOI:10.1016/j.resmic.2014.10.003]
7. LahtiP, Heikinheimo A, Johansson T, et al. Clostridium perfringens type A strains carrying a plasmid-borne enterotoxin gene (genotype IS1151-cpe or IS1470-like-cpe) as a common cause of food poisoning. J Clin Microbiol.2008; 46(1):371-373. [DOI:10.1128/JCM.01650-07]
8. Ma M, Li J , McClane BA. Genotypic and phenotypic characterization of Clostridium perfringens isolates from Darmbrand cases in post-World War II Germany. Infect immun.2012; 80(12):4354-4363. [DOI:10.1128/IAI.00818-12]
9. Miyamoto K, Li J , McClane BA. Enterotoxigenic Clostridium perfringens: detection and identification. Microbes Environ. 2012;27(4):343-349. [DOI:10.1264/jsme2.ME12002]
10. Abdelrahim AM, Radomski N, Delannoy S, et al. Large-scale genomic analyses and toxinotyping of Clostridium perfringens implicated in foodborne outbreaks in France. Front Microbiol. 2019; 10:777. [DOI:10.3389/fmicb.2019.00777]
11. Lindström M, Heikinheimo A, Lahti P, Korkeala H. Novel insights into the epidemiology of Clostridium perfringens type A food poisoning. Food Microbiol. 2011;28(2):192-8. [DOI:10.1016/j.fm.2010.03.020]
12. Erol I, Goncuoglu M, Ayaz N, et al. Molecular typing of Clostridium perfringens isolated from turkey meat by multiplex PCR. Lett Appl Microbiol.2008, 47(1): 31-34. [DOI:10.1111/j.1472-765X.2008.02379.x]
13. Ossiprandi MC, Zerbini L. Molecular evaluation of the enterotoxigenicity of Clostridium difficile and Clostridium perfringens swine isolates by PCR assays. Adv Microbiol,2013; 3: 154-59. [DOI:10.4236/aim.2013.32024]
14. Miki Y, Miyamoto K, Kaneko-Hirano I,et al. Prevalence and characterization of enterotoxin gene-carrying Clostridium perfringens isolates from retail meat products in Japan. Appl Environ Microbiol. 2008;74(17):5366-72. [DOI:10.1128/AEM.00783-08]
15. Sagoo S k, Little CL, Greenwood M, et al. Assessment of the microbiological safety of dried spices and herbs from production and retail premises in the United Kingdom. Food microbiol.2009; 26(1) 39-43. [DOI:10.1016/j.fm.2008.07.005]
16. Miyamoto K, Li J, McClane BA. Enterotoxigenic Clostridium perfringens: detection and identification. Microbes Environ. 2012;27(4):343-9. [DOI:10.1264/jsme2.ME12002]
17. Kaneko I, Miyamoto K, Mimura K, et al.Detection of enterotoxigenic Clostridium perfringens in meat samples by using molecular methods. Appl Environ Microbiol.2011; 77(21):7526-32. [DOI:10.1128/AEM.06216-11]
18. Poursoltani M, Mohsenzadeh M, Razmyar J. Toxinotyping of Clostridium perfringens strains isolated from packed chicken portions. Iranian J Medl Microbiol.2014; 8(1):9-17.
19. Zandi E, Mohammadabadi M, Ezzatkhah M, et al. Typing of toxigenic isolates of Clostridium perfringensby multiplex PCR in ostrich. Iranian J Appl Anim Sci.2014; 4(4):795-801.

بازنشر اطلاعات
Creative Commons License این مقاله تحت شرایط Creative Commons Attribution-NonCommercial 4.0 International License قابل بازنشر است.

کلیه حقوق این وب سایت متعلق به Journal of Advances in Medical and Biomedical Research می باشد.

طراحی و برنامه نویسی : یکتاوب افزار شرق

© 2024 CC BY-NC 4.0 | Journal of Advances in Medical and Biomedical Research

Designed & Developed by : Yektaweb