دوره 29، شماره 137 - ( 7-1400 )                   جلد 29 شماره 137 صفحات 323-317 | برگشت به فهرست نسخه ها


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Keshavarzi S, Sepehrimanesh M, Mirzaei B, Koohi-Hosseinabadi O, Sardari Z, Sajjadpur M M. Antimicrobial Effects of Aqueous and Hydro-Alcoholic Plantago psyllium leaf extracts on the Experimental Infection of Helicobacter pylori in a Rat Model. J Adv Med Biomed Res 2021; 29 (137) :317-323
URL: http://journal.zums.ac.ir/article-1-6203-fa.html
Antimicrobial Effects of Aqueous and Hydro-Alcoholic Plantago psyllium leaf extracts on the Experimental Infection of Helicobacter pylori in a Rat Model. Journal of Advances in Medical and Biomedical Research. 1400; 29 (137) :317-323

URL: http://journal.zums.ac.ir/article-1-6203-fa.html


چکیده:   (120453 مشاهده)

Background and Objective: Plantago psyllium has long been utilised as a medicinal agent worldwide. The current study aims to evaluate the in vivo antimicrobial effects of aqueous P. psyllium and a hydro-alcoholic extract of P. psyllium on Helicobacter pylori as well as the effects of these extracts on liver and kidney in a rat model.
Materials and Methods: A total number of 50 female Sprague Dawley rats were experimentally infected (Except for the negative control group). The rats were divided into five groups of 10. The groups were treated by Aqueous and Hydro-Alcoholic P. psyllium leaf extracts. Subsequently, H. pylori antigens which were present in the in rats’ stool were measured using a serological assay. To diagnose the pathogenicity of the kidney and liver, blood samples which had been taken before and after treatment, were tested for renal and hepatic enzymes by Analyzer Electrolyte and Sysmex KX-21N.
Results: Following the amoxicillin treatment, the levels of aqueous and hydro-alcoholic extracts of the treated rats were compared with those of the control group, and a significant difference was observed (P<0.05). Antigen decrease was observed in all of the groups treated with amoxicillin (P=0.0004), aqueous, and hydro-alcoholic extracts (P<0.05). Antigen decrease in the stool was observed in all groups, which indicates that treatment with the herbal extract could affect the infected rats.
Conclusion: P. psyllium hydro-alcoholic extract can be applied as a selective treatment for H. pylori infection. Hopefully, experiments suggested that the mentioned extracts could positively affect the process of disease recovery.

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نوع مطالعه: مقاله پژوهشی | موضوع مقاله: Life science
دریافت: 1399/9/16 | پذیرش: 1399/12/14 | انتشار: 1400/5/10

فهرست منابع
1. Magalhães Queiroz DM, Luzza F. Epidemiology of Helicobacter pylori infection. Helicobacter. 2006;11:1-5. [DOI:10.1111/j.1478-405X.2006.00429.x]
2. Lawrence RS, Durch JS, Stratton KR. Vaccines for the 21st century: a tool for decisionmaking: National Academies Press; 2001.
3. Farzi N, Yadegar A, Sadeghi A, et al. High prevalence of antibiotic resistance in Iranian Helicobacter pylori isolates: importance of functional and mutational analysis of resistance genes and virulence genotyping. J Clin Med. 2019;8(11):2004. [DOI:10.3390/jcm8112004]
4. Zhang XL, Jiang AM, Ma ZY, et al. The synthetic antimicrobial peptide pexiganan and its nanoparticles (PNPs) exhibit the anti-Helicobacter pylori activity in vitro and in vivo. Molecules. 2015;20(3):3972-85. [DOI:10.3390/molecules20033972]
5. Ahmadi R, Kalbasi-Ashtari A, Oromiehie A, Yarmand M-S, Jahandideh F. Development and characterization of a novel biodegradable edible film obtained from psyllium seed (Plantago ovata Forsk). J Food Engineer. 2012;109(4):745-51. [DOI:10.1016/j.jfoodeng.2011.11.010]
6. Hajhashemi V, Ghannadi A, Hajiloo M. Analgesic and anti-inflammatory effects of Rosa damascena hydroalcoholic extract and its essential oil in animal models. Iran J Pharmaceuti Res. 2010;9(2):163.
7. Hajhashemi V, Sajjadi SE, Heshmati M. Anti-inflammatory and analgesic properties of Heracleum persicum essential oil and hydroalcoholic extract in animal models. J Ethnopharmacol. 2009;124(3):475-80. [DOI:10.1016/j.jep.2009.05.012]
8. Ladjevardi ZS, Gharibzahedi SMT, Mousavi M. Development of a stable low-fat yogurt gel using functionality of psyllium (Plantago ovata Forsk) husk gum. Carbohydrate Polymers. 2015;125:272-80. [DOI:10.1016/j.carbpol.2015.02.051]
9. Abou Azar S, Wehbe MR, Jamali S, Hallal A. Small bowel obstruction secondary to a Metamucil bezoar: Case report and review of the literature. Case Rep Surg. 2017;2017: 2702896. [DOI:10.1155/2017/2702896]
10. Yousef-Nezhad H, Hejazi N. Anti-bacterial properties of herbs against Helicobacter Pylori infection: A review. Int J Nutr Sci. 2017;2(3):126-33.
11. Sepehri Z, Nasiri AA, Azizi A, Golshani F, Sargazi A. Antibacterial effects of ethanol extract of Plantago psyllium on antibiotic-resistant Salmonella typhimurium. J Zabol Univ Med Sci Health Serv. 2016;7(4):1-8.
12. Graham DY, Malaty HM, Evans DG, Evans Jr DJ, Klein PD, Adam E. Epidemiology of Helicobacter pylori in an asymptomatic population in the United States: effect of age, race, and socioeconomic status. Gastroenterol. 1991;100(6):1495-501. [DOI:10.1016/0016-5085(91)90644-Z]
13. Lieber CS. Alcohol and the liver: metabolism of alcohol and its role in hepatic and extrahepatic diseases. Mt Sinai J Med . 2000;67(1):84-94
14. McColl KE. Helicobacter pylori infection. New Eng J Med. 2010;362(17):1597-604. [DOI:10.1056/NEJMcp1001110]
15. Pastukh N, Binyamin D, On A, Paritsky M, Peretz A. GenoType® Helico DR test in comparison with histology and culture for Helicobacter pylori detection and identification of resistance mutations to clarithromycin and fluoroquinolones. Helicobacter. 2017;22(6):e12447. [DOI:10.1111/hel.12447]
16. Trivedi MK, Gangwar M, Mondal SC, Jana S. Immunomodulatory properties and biomarkers characterization of novel Withania somnifera based formulation supplemented with minerals in Sprague Dawley rats. Orient Pharm Experiment Med. 2017;17(1):59-69. [DOI:10.1007/s13596-016-0255-5]
17. Mehmood MH, Aziz N, Ghayur MN, Gilani AH. Pharmacological basis for the medicinal use of psyllium husk (Ispaghula) in constipation and diarrhea. Digest Dis Sci. 2011;56(5):1460-71. [DOI:10.1007/s10620-010-1466-0]
18. Radfar MH, Zarandi MB, Bamorovat M, Kheirandish R, Sharifi I. Hematological, biochemical and pathological findings in goats naturally infection with Cysticercus tenuicollis. J Parasit Dis. 2014;38(1):68-72. [DOI:10.1007/s12639-012-0188-y]
19. Graham DY, Fischbach L. Helicobacter pylori treatment in the era of increasing antibiotic resistance. Gut. 2010;59(8):1143-53. [DOI:10.1136/gut.2009.192757]
20. Mobin M, Rizvi M. Polysaccharide from Plantago as a green corrosion inhibitor for carbon steel in 1 M HCl solution. Carbohydrate Polymer. 2017;160:172-83. [DOI:10.1016/j.carbpol.2016.12.056]
21. Pouladi S, Bagherpour Borazjani A, Motamed N, et al. A Survey on the effect of psyllium on serum levels of triglycerid and lipoproteins. Iran South Med J. 2009;11(2):139-46.
22. Singh B. Psyllium as therapeutic and drug delivery agent. Int J Pharmaceut. 2007;334(1-2):1-14. [DOI:10.1016/j.ijpharm.2007.01.028]
23. Hetland G, Samuelsen A, Loslash V, et al. Protective effect of Plantago major L. Pectin polysaccharide against systemic Streptococcus pneumoniae infection in mice. Scand J Immunol. 2000;52(4):348-55. [DOI:10.1046/j.1365-3083.2000.00793.x]
24. Bokaeian M, Fakheri BA, Mohasseli T, Saeidi S. Antibacterial activity of silver nanoparticles produced by plantago ovata seed extract against antibiotic resistant Staphylococcus aureus. Int J Infect. 2015;2(1):e22854. [DOI:10.17795/iji-22854]
25. Taheri A, Seyfan A, Jalalinezhad S, Nasery F. Antibacterial effect of Myrtus communis hydro-alcoholic extract on pathogenic bacteria. Zahedan J Res Med Sci. 2013;15(6):19-24.

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