دوره 31، شماره 147 - ( 5-1402 )                   جلد 31 شماره 147 صفحات 397-391 | برگشت به فهرست نسخه ها

XML English Abstract Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Heidari A, Meftahi G, Bahrami F, Bahari Z, Zarei Mahmoudabadi A, Fallah Huseini H et al . Anti-Adipogenic Effects of Eryngium billardieri Extract during 3T3-L1 Adipocyte Differentiation. J Adv Med Biomed Res 2023; 31 (147) :391-397
URL: http://journal.zums.ac.ir/article-1-6895-fa.html
Anti-Adipogenic Effects of Eryngium billardieri Extract during 3T3-L1 Adipocyte Differentiation. Journal of Advances in Medical and Biomedical Research. 1402; 31 (147) :391-397

URL: http://journal.zums.ac.ir/article-1-6895-fa.html

Anti-Adipogenic Effects of Eryngium billardieri Extract during 3T3-L1 Adipocyte Differentiation
چکیده:   (1249 مشاهده)

Background and Objective: Obesity is a major public health problem whose prevalence has constantly increased worldwide. Obesity is a risk factor for several diseases such as type 2 diabetes, fatty liver disease, hyperlipidemia, cardiovascular diseases, and cancer, all of which reduce the quality of life and life expectancy. This study investigated anti-adipogenic effects of Eryngium billardierei (Ery) extract.
Materials and Methods: The nontoxic concentration of Ery extract was estimated via the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazoliu bromide (MTT) assay. The differentiation of mouse-derived 3T3-L1 preadipocytes into mature adipocytes was induced in the presence or absence of Ery extract. Then, the accumulation of lipid droplets was identified by Oil Red O staining. Triacylglycerol level and glycerol-3phosphate dehydrogenase (GPDH) activity were also measured.
Results: The MTT assay showed that 3 mg/mL of the Ery extract can be a nontoxic dose for other analyses. Oil Red O staining demonstrated a reduction of lipid droplets in the extract-treated adipocyte 3T3-L1 compared to the non-treated group. A significant decrease of triacylglycerol level in the treated 3T3-L1 adipocyte (0.32±0.03 mgTG/mg protein) compared with the nontreated group (0.78±0.05 mgTG/mg protein) (P<0.01), and a significant decrease of glycerol-3-phosphate dehydrogenase (GPDH) activity in the 3T3-L1adipocyte treated (15.3±2.1 U/ml) compared to the non-treated group (30.2±3.9 U/ml) (P<0.05) confirmed the inhibitory action of Ery extract on adipocyte differentiation.
Conclusion: Ery extract, as a safe herbal extract, has anti-adipogenic activity and merits more investigation as a candidate for developing an anti-obesity drug or supplement.

متن کامل [PDF 767 kb]   (596 دریافت)    
نوع مطالعه: مقاله پژوهشی | موضوع مقاله: Medical Biology
دریافت: 1401/10/1 | پذیرش: 1402/1/9 | انتشار: 1402/7/5
فهرست منابع
1. Friedman JM. Obesity in the new millennium. Nature. 2000;404, 632-34. [DOI:10.1038/35007504] [PMID]
2. Blüher M. Obesity: global epidemiology and pathogenesis. Nat Rev Endocrinol. 2019;15(5):288-98. [DOI:10.1038/s41574-019-0176-8] [PMID]
3. Collaborators GB, Afshin A, Forouzanfar MH, et al. Health effects of overweight and obesity in 195 countries over 25 years. New Eng J Med. 2017; 377: 13-27. [DOI:10.1056/NEJMoa1614362] [PMID] [PMCID]
4. Jakab J, Miškić B, Mikšić Š,et al. Adipogenesis as a potential anti-obesity target: A review of pharmacological treatment and natural products.Diabetes Metab Syndr Obes. 2021;14:67-83. [DOI:10.2147/DMSO.S281186] [PMID] [PMCID]
5. Williams DM, Nawaz A, Evans M. Drug therapy in obesity: A review of current and emerging treatments. Diabetes Ther. 2020: 11(6):1199-1216. [DOI:10.1007/s13300-020-00816-y] [PMID] [PMCID]
6. Kapoor K, Madaan R, Kumar S, Bala R, Walia RP. Role of natural products in the treatment of obesity: Nanotechnological Perspectives. Curr Drug Metab. 2021;22(6):451-80. [DOI:10.2174/1389200222666210324150738] [PMID]
7. Tak YJ, Lee SY. Long-term efficacy and safety of anti-obesity treatment: Where do we stand? .Curr Obes Rep. 2021;10(1):14-30. [DOI:10.1007/s13679-020-00422-w] [PMID] [PMCID]
8. Rosen ED, MacDougald OA. Adipocyte differentiation from the inside out. Nat Rev Mol Cell Biol. 2006;7:885-96. [DOI:10.1038/nrm2066] [PMID]
9. Ping W, Zushang S, Wei Y, Guangrui D, Shiyou L. Phytochemical constituents and pharmacological activities of Eryngium L. (Apiaceae). Pharmaceutical Crops.2012, 3: 99-120. [DOI:10.2174/2210290601203010099]
10. Kikowska M, Chanaj-Kaczmarek J, Derda M, et al. The evaluation of phenolic acids and flavonoids content and antiprotozoal activity of Eryngium species Biomass produced by Biotechnological Methods. Molecules. 2022;27(2):363. [DOI:10.3390/molecules27020363] [PMID] [PMCID]
11. Khani S, Abdollahi M, Asadi Z, et al. Hypoglycemic, hepatoprotective, and hypolipidemic effects of hydroalcoholic extract of Eryngium billardieri root on nicotinamide/streptozotocin-induced type II diabetic rats. Res Pharm Sci. 2021;16(2):193-202. [DOI:10.4103/1735-5362.310526] [PMID] [PMCID]
12. Roshanravan N, Asgharian P, Dariushnejad H, et al. Eryngium Billardieri induces apoptosis via Bax gene expression in pancreatic cancer cells. Adv Pharm Bull. 2018;8(4):667-74. [DOI:10.15171/apb.2018.075] [PMID] [PMCID]
13. Paşayeva L, Köngül E, Geylan R, Karatoprak GŞ, Tugay O. Analysis of the cytotoxic effects of Eryngium billardieri delar. Extracts on MCF7 Cell Line. Proceedings. 2017; 1(10):1055. [DOI:10.3390/proceedings1101055]
14. Liu H, Song Y, Zhang X. Determination of total flavonoids in leek by AlCl 3 colorimetric assay. Chem Eng Transact. 2017;59:775-80.
15. Park E, Lee CG, Kim J, et al. Antiobesity effects of Gentiana lutea extract on 3T3-L1 preadipocytes and a high-fat diet-induced mouse model. .Molecules. 2020;25(10):2453. https://doi.org/10.3390/molecules25102350 [DOI:10.3390/molecules25102453]
16. Roh C, Jung U. Screening of crude plant extracts with anti-obesity activity. Int J Mol Sci 2012;13(2):1710-9. [DOI:10.3390/ijms13021710] [PMID] [PMCID]
17. Park MJ, Song JH, Shon MS,et al. Anti-adipogenic effects of ethanol extracts prepared from selected medicinal herbs in 3T3-L1 cells. Prev Nutr Food Sci. 2016;21(3):227-35. [DOI:10.3746/pnf.2016.21.3.227] [PMID] [PMCID]
18. Erdem SA, Nabavi SF, Orhan IE, Daglia M, Izadi M, Nabavi SM. Blessings in disguise: a review of phytochemical composition and antimicrobial activity of plants belonging to the genus Eryngium. Daru. 2015;23:53. [DOI:10.1186/s40199-015-0136-3] [PMID] [PMCID]
19. Pérez-Muñoz EP, Antunes-Ricardo M, Martínez-Ávila M, Guajardo-Flores D. Eryngium species as a potential ally for treating metabolic syndrome and diabetes. Front Nutr. 2022;9:878306. [DOI:10.3389/fnut.2022.878306] [PMID] [PMCID]
20. GBD 2015 Obesity Collaborators. Health effects of overweight and obesity in 195 countries over 25 years. N Engl J Med. 2017;377(1):13-27. [DOI:10.1056/NEJMoa1614362] [PMID] [PMCID]
21. Thomas SS, Eom J, Sung NY, Kim DS, Cha YS, Kim KA. Inhibitory effect of ethanolic extract of Abeliophyllum distichum leaf on 3T3-L1 adipocyte differentiation. Nutr Res Pract. 2021;15(5):555-567. [DOI:10.4162/nrp.2021.15.5.555] [PMID] [PMCID]
22. Telapolu S, Kalachavedu M, Punnoose AM, Bilikere D. MD-1, a poly herbal formulation indicated in diabetes mellitus ameliorates glucose uptake and inhibits adipogenesis - an in vitro study. BMC Complement Altern Med.2018;18(1):113. [DOI:10.1186/s12906-018-2177-x] [PMID] [PMCID]
23. Khani S, Abdollahi M, Asadi Z, et al. Hypoglycemic, hepatoprotective, and hypolipidemic effects of hydroalcoholic extract of eryngium billardieri root on nicotinamide/streptozotocin-induced type II diabetic rats. Res Pharm Sci. (2021) 16:193-202. [DOI:10.4103/1735-5362.310526] [PMID] [PMCID]
24. Noriega-Cisneros R, Peña-Montes DJ, Huerta-Cervantes M, et al. Eryngium carlinae ethanol extract corrects lipid abnormalities in wistar rats with experimental diabetes. J Med Food. 2020;23(8):827-33. [DOI:10.1089/jmf.2019.0189] [PMID]
25. Noriega-Cisneros R, Ortiz-Ávila O, Esquivel-Gutiérrez E, et al. Hypolipidemic activity of Eryngium carlinae on streptozotocin-induced diabetic rats. Biochem Res Int. 2012; 2012:603501. [DOI:10.1155/2012/603501] [PMID] [PMCID]
26. Wise L, Green H. Participation of one isozyme of cytosolic glycerophosphatedehydrogenase in the adipose conversion of 3T3 cells. J Biol Chem.1979; 254: 273-75. [DOI:10.1016/S0021-9258(17)37913-9] [PMID]
27. Taghipour R, Mahmoudabadi AZ, Jangravi Z. Evaluation of the effect of erynjium alcoholic extract on increasing insulin sensitivity: By evaluating hyperglycemia and dyslipidemia in an animal model of insulin resistance. J North Khorasan Univ Med Sci. 2021;13(3):43-50. [DOI:10.52547/nkums.13.3.43]
بازنشر اطلاعات
Creative Commons License این مقاله تحت شرایط Creative Commons Attribution-NonCommercial 4.0 International License قابل بازنشر است.

کلیه حقوق این وب سایت متعلق به Journal of Advances in Medical and Biomedical Research می باشد.

طراحی و برنامه نویسی : یکتاوب افزار شرق

© 2024 CC BY-NC 4.0 | Journal of Advances in Medical and Biomedical Research

Designed & Developed by : Yektaweb