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Ethics code: IR.KUMS.REC.1399.1158
چکیده: (529 مشاهده)
Background & Objective: Glioblastoma multiforme (GBM) is the most aggressive primary brain tumor, characterized by extensive infiltration into surrounding brain tissue. Due to the limited efficacy of current therapeutic interventions, identifying novel compounds capable of suppressing GBM progression is a critical research priority. Taraxasterol, a pentacyclic triterpene extracted from Taraxacum officinale, has demonstrated anticancer activity in several tumor models. This study investigated the effects of taraxasterol on the migration, invasion, and adhesion of human U87MG glioblastoma cells and evaluated its impact on MMP-2 and MMP-9 expression.
Materials & Methods: U87MG cells were treated with escalating concentrations of taraxasterol (1–128 μmuμM) over 24, 48, and 72 h. Cell viability was assessed using MTT, trypan blue exclusion, and LDH release assays. Cell migration, invasion, and adhesion were evaluated via wound-healing, Transwell, and adhesion assays, respectively. The mRNA expression levels of MMP-2 and MMP-9 were quantified using real-time PCR.
Results: Taraxasterol significantly reduced U87MG cell viability in a dose- and time-dependent manner. Treatment at the IC50 concentration markedly inhibited cell migration (~70%), invasion (>55%), and adhesion (~25%) compared with untreated controls (p < 0.05). Furthermore, taraxasterol significantly downregulated MMP-2 and MMP-9 mRNA expression levels, reducing their expression to approximately 25% and 30% of control values, respectively (p < 0.01).
Conclusion: Taraxasterol effectively suppresses the migratory, invasive, and adhesive properties of glioblastoma cells, potentially through the downregulation of MMP-2 and MMP-9 expression. Consequently, taraxasterol represents a promising candidate for the development of adjunctive therapeutic strategies aimed at limiting glioblastoma invasiveness.
Materials & Methods: U87MG cells were treated with escalating concentrations of taraxasterol (1–128 μmuμM) over 24, 48, and 72 h. Cell viability was assessed using MTT, trypan blue exclusion, and LDH release assays. Cell migration, invasion, and adhesion were evaluated via wound-healing, Transwell, and adhesion assays, respectively. The mRNA expression levels of MMP-2 and MMP-9 were quantified using real-time PCR.
Results: Taraxasterol significantly reduced U87MG cell viability in a dose- and time-dependent manner. Treatment at the IC50 concentration markedly inhibited cell migration (~70%), invasion (>55%), and adhesion (~25%) compared with untreated controls (p < 0.05). Furthermore, taraxasterol significantly downregulated MMP-2 and MMP-9 mRNA expression levels, reducing their expression to approximately 25% and 30% of control values, respectively (p < 0.01).
Conclusion: Taraxasterol effectively suppresses the migratory, invasive, and adhesive properties of glioblastoma cells, potentially through the downregulation of MMP-2 and MMP-9 expression. Consequently, taraxasterol represents a promising candidate for the development of adjunctive therapeutic strategies aimed at limiting glioblastoma invasiveness.
فهرست منابع
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