دوشنبه 26 مرداد 1405
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Ethics code: IR.KUMS.MED.REC.1403.232
چکیده: (5 مشاهده)
Background: Diabetes mellitus remains a global health challenge associated with oxidative stress and impaired insulin signaling. Natural compounds with dual antioxidant and enzyme inhibitory potential are of great interest as complementary therapeutic agents. Spirulina platensis, a blue-green microalga rich in proteins, polyphenols, and phycocyanin, has been reported to exhibit diverse bioactivities.
Objective: This study aimed to investigate the in vitro antioxidant and antidiabetic effects of Spirulina platensis extract, focusing on its enzymatic inhibition and modulation of insulin gene expression in pancreatic β-cells.
Methods: Ethanolic extracts of Spirulina platensis were assessed for free radical scavenging using DPPH and ABTS assays. The inhibitory activity on α-amylase, α-glucosidase, and protein tyrosine phosphatase-1B (PTP1B) was evaluated spectrophotometrically. Cytotoxicity and insulin secretion were analyzed in RIN-5F pancreatic β-cells, followed by qPCR assessment of INS I, INS II, IRS-I, IRS-II, and IRS-III gene expression.
Results: The extract displayed strong, dose-dependent antioxidant activity (p ≤ 0.05) and significant inhibition of α-amylase, α-glucosidase, and PTP1B enzymes. No cytotoxicity was observed below 100 µg/mL. Treatment with 20–60 µg/mL enhanced insulin secretion and markedly upregulated insulin-related gene expression in β-cells.
Conclusion: Spirulina platensis demonstrates potent in vitro antioxidant and antidiabetic effects through enzymatic inhibition and stimulation of insulin gene expression. These findings highlight Spirulina as a promising natural candidate for developing safe, plant-based antidiabetic agents.
Objective: This study aimed to investigate the in vitro antioxidant and antidiabetic effects of Spirulina platensis extract, focusing on its enzymatic inhibition and modulation of insulin gene expression in pancreatic β-cells.
Methods: Ethanolic extracts of Spirulina platensis were assessed for free radical scavenging using DPPH and ABTS assays. The inhibitory activity on α-amylase, α-glucosidase, and protein tyrosine phosphatase-1B (PTP1B) was evaluated spectrophotometrically. Cytotoxicity and insulin secretion were analyzed in RIN-5F pancreatic β-cells, followed by qPCR assessment of INS I, INS II, IRS-I, IRS-II, and IRS-III gene expression.
Results: The extract displayed strong, dose-dependent antioxidant activity (p ≤ 0.05) and significant inhibition of α-amylase, α-glucosidase, and PTP1B enzymes. No cytotoxicity was observed below 100 µg/mL. Treatment with 20–60 µg/mL enhanced insulin secretion and markedly upregulated insulin-related gene expression in β-cells.
Conclusion: Spirulina platensis demonstrates potent in vitro antioxidant and antidiabetic effects through enzymatic inhibition and stimulation of insulin gene expression. These findings highlight Spirulina as a promising natural candidate for developing safe, plant-based antidiabetic agents.
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