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Volume 25, Issue 113 (8-2017)
J Adv Med Biomed Res 2017, 25(113): 122-134 |
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Danafar H, Kheiri Manjili H R, Attari E, Sharafi A. Investigation of Drug Delivery of Rattle-Structured Gold Nanorod-Mesoporous Silica Nanoparticles Core–Shell as Curcumin Carrier and Their Effect on MCF7 and 4T1 Cell Lines. J Adv Med Biomed Res 2017; 25 (113) :122-134
URL: http://journal.zums.ac.ir/article-1-4803-en.html
URL: http://journal.zums.ac.ir/article-1-4803-en.html
1- Dept, of Medicinal Chemistry, School of Pharmacy Zanjan University of Medical Sciences Zanjan, Iran
2- Dept. of Pharmaceutical Nanotechnology, School of Pharmacy, Zanjan University of Medical Sciences, Zanjan, Iran
3- Student Researches Committee, School of Pharmacy, Zanjan University of Medical Sciences, Zanjan, Iran
4- Dept. of Biotechnology, Faculty of Pharmacy, Zanjan University of Medical Sciences, Zanjan, Iran
2- Dept. of Pharmaceutical Nanotechnology, School of Pharmacy, Zanjan University of Medical Sciences, Zanjan, Iran
3- Student Researches Committee, School of Pharmacy, Zanjan University of Medical Sciences, Zanjan, Iran
4- Dept. of Biotechnology, Faculty of Pharmacy, Zanjan University of Medical Sciences, Zanjan, Iran
Abstract: (153686 Views)
Background and Objective: Curcumin is a polyphenolic anti-cancer and anti-inflammatory agent and can be used both orally and by injection. In this study, nanoparticles of silica mesopore- gold nano were synthesized as a new drug delivery system. For this aim, gold nanoparticles as a promising system for effective drug delivery of curcumin was used and nano gold were delivered to breast cancer cells .
Materials and Methods: The synthetic nanoparticles characteristics were determined with different techniques such as transmission electron microscopy (TEM), nitrogen gas desorption, absorption analysis and dynamic light scattering techniques (DLS). The anti-cancer effects of this system were evaluated in cell culture, using MTT cytotoxicity test. The efficiency of this system was tested on human breast cancer (MCF-7) and 4T1 cell lines.
Results: The mean size of nano system was less than 30 nm, similar to the data obtained from Dynamic light scattering technique. The amount of curcumin loading in nano- system was about 80 percent. Isotherm - nitrogen adsorption AuNR@msio2 and CUR @ AuNR @ msio2 show that specific area of nano-Au @ msio2 was about -1834 m2g but by creating medication this particular area decrease to 234 m2g-1. IC50 for curcumin loaded mesoporous silica nanoparticles for MCF-7 at the time of 48 hours and 4T1 were 19.5 and 14.6 micro molar respectively. The survival rate of the cells after treatment with different concentrations did not change considerably in the nano structures which demonstrate the lack of toxicity of nanoparticles.
Conclusion: The nanoparticle caused apoptosis in breast cancer cells more than free drug.
Materials and Methods: The synthetic nanoparticles characteristics were determined with different techniques such as transmission electron microscopy (TEM), nitrogen gas desorption, absorption analysis and dynamic light scattering techniques (DLS). The anti-cancer effects of this system were evaluated in cell culture, using MTT cytotoxicity test. The efficiency of this system was tested on human breast cancer (MCF-7) and 4T1 cell lines.
Results: The mean size of nano system was less than 30 nm, similar to the data obtained from Dynamic light scattering technique. The amount of curcumin loading in nano- system was about 80 percent. Isotherm - nitrogen adsorption AuNR@msio2 and CUR @ AuNR @ msio2 show that specific area of nano-Au @ msio2 was about -1834 m2g but by creating medication this particular area decrease to 234 m2g-1. IC50 for curcumin loaded mesoporous silica nanoparticles for MCF-7 at the time of 48 hours and 4T1 were 19.5 and 14.6 micro molar respectively. The survival rate of the cells after treatment with different concentrations did not change considerably in the nano structures which demonstrate the lack of toxicity of nanoparticles.
Conclusion: The nanoparticle caused apoptosis in breast cancer cells more than free drug.
Type of Study: Clinical Trials |
Received: 2017/08/27 | Accepted: 2017/08/27 | Published: 2017/08/27
Received: 2017/08/27 | Accepted: 2017/08/27 | Published: 2017/08/27
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