Background and Objective: Fatty liver disease occurs due to disturbances in lipid metabolism (increased fat synthesis and halting of catabolism) which eventually leads to the accumulation of large amounts of fat (triglycerides) in liver cells, that if left untreated lead to inflammation and cirrhosis. This study aimed to investigate the effect of concomitant use of HESA-A and atorvastatin on biochemical parameters in non-alcoholic fatty liver disease compared with atorvastatin alone.

Methods and Materials: 28 rats weighing 180±20 g were randomly divided into two groups: a control group (n = 7) with standard diet and a HFD (high-fat diet) group (n=21) which were fed for 8 weeks. To confirm the induction of fatty liver; controls along with seven rats from the HFD group were killed. The remaining 14 animals were divided into two groups. The first group received atorvastatin and the second group received HESA-A in combination with atorvastatin daily for 30 days. Finally, biochemical parameters (glucose, lipid profile and liver enzymes) were measured and histological studies were performed on the liver samples.

Results: Biochemical parameters in the HESA-A combined with atorvastatin group were significantly decreased in comparison to the atorvastatin group (p<0.01). The histopathological study of liver tissue showed a significant reduction in the amount of fat tissue and conversion of fatty liver from grade ІІІ to grade І.

Conclusion: According to the results of this study, the use of HESA-A as a complementary treatment for fatty liver disease without side effects can be integral role in reducing the incidence of non-alcoholic fatty liver disease, especially in severe cases.

Background and Objective: Datura stramonium L. is a medicinal herb from the family of Solanaceae. It has been used in herbal remedies for promoting health and treating several diseases. The current study was set up to compare the effects of Datura stramonium L. extract on the naloxone-precipitated opiate-withdrawal in mice.
Materials and Methods: Male BALB/c mice (30–35 g, n = 40) were arbitrarily separated into 4 groups. The control group received morphine and normal saline and other groups received three doses of D. stramonium extract (10, 20, or 30 mg/kg, intraperitoneally, i.p.). Physically dependent was made by the administration of morphine in increasing doses (50-75 mg/kg, i.p.). The withdrawal signs were elicited by intraperitoneal injections of naloxone (5 mg/kg) 2 h after the last injection of morphine.
Results: Administration of D. stramonium extract in doses of 20 and 30 mg/kg markedly diminished the jumping numbers compared to the control group (P<0.05). All three doses of D. stramonium extract could significantly suppress the increase in climbing (P<0.05, P<0.001, and P<0.001, respectively) and diarrhea (P<0.001). D. stramonium in higher doses (20 or 30 mg/kg) significantly decreased rearing and itching (P<0.001).
Conclusion: The study findings suggest that D. stramonium extract is effective in alleviating the signs of morphine withdrawal. Additional research is needed to determine the exact mechanisms underlying D. stramonium for inhibiting morphine withdrawal syndrome.