Volume 18, Issue 72 (11-2010)                   J Adv Med Biomed Res 2010, 18(72): 42-51 | Back to browse issues page

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Tayarani-Najaran Z, Parsaee H, Mousavi S H. Study of High Glucose-Induced Toxicity and Reactive Oxygen Species Production and the Protective Effect of Saffron Extract in PC12 Cells. J Adv Med Biomed Res 2010; 18 (72) :42-51
URL: http://journal.zums.ac.ir/article-1-1220-en.html
1- Pharmacological Research Center of Medicinal Plants, Dept. of Pharmacology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
2- Medical Toxicology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran , mousavih@mumslac.ir
Abstract:   (177505 Views)

Background and Objective: Diabetic neuropathy is one of the most frequent complications of diabetes. Despite some studies, the exact mechanism of glucose neurotoxicity has not been fully elucidated. Increased reactive oxygen species (ROS) has been proposed as a possible mechanism. Crocus sativus L. (saffron) has been known as a source of antioxidants. Therefore, neuroprotective effect of saffron extract was studied in glucose-induced neurotoxicity, using PC12 cells as a suitable in vitro model of diabetic neuropathy. Materials and Methods: Cell viability was quantitated by MTT assay. ROS was measured using DCF-DA by flow cytometry analysis. Results: The result showed that glucose reduced the cell viability of PC12 cells. Saffron extract and GSH could decrease this toxicity. Glucose toxicity was consistent with increased ROS production which was reduced by saffron and GSH pre-treatment. Conclusion: These results suggest that the glucose-induced cell toxicity could be mediated through the generation of ROS. Due to antioxidant effects of Saffron, it could be considered in diabetic neuropathy treatment in animal models.

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Type of Study: Original Research Article |
Received: 2010/08/31 | Accepted: 2014/06/23 | Published: 2014/06/23

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