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Ghaderinia P, Shapouri R, Rostamizadeh K, Khodavandi A, Mahdavi M. Capsular K-antigen-PLGA Nano conjugated Vaccine against Klebsiella pneumoniea pneumoniae K2O1 Infection. J Adv Med Biomed Res 2022; 30 (138) :73-74
URL: http://journal.zums.ac.ir/article-1-6359-en.html
Capsular K-antigen-PLGA Nano conjugated Vaccine against Klebsiella pneumoniea pneumoniae K2O1 Infection
Parivash Ghaderinia1 , Reza Shapouri2 , Kobra Rostamizadeh * 3, Alireza Khodavandi4 , Mehdi Mahdavi5
1- Dept. of Microbiology, College of Science agriculture and modern technology, Shiraz Branch, Islamic Azad University, Shiraz, Iran
2- Dept. of Microbiology, Zanjan Branch, Islamic Azad University, Zanjan, Iran
3- Zanjan Pharmaceutical Nanotechnology Research Center, Zanjan University of Medical Science, Zanjan, Iran , rostamizadeh@gmail.com
4- Dept. of Biology, Gachsaran Branch, Islamic Azad University, Gachsaran, Iran
5- Recombinant Vaccine Research Center, Tehran University of Medical Sciences, Tehran, Iran
Abstract:   (110741 Views)

Klebsiella pneumoniae is the most common pathogenic bacterium in the genus Klebsiella (1). The aim of this study was to use Polylactic-co-glycolic acid (PLGA) nanoparticles in vaccine design of the capsule antigen of Klebsiella pneumoniae K2O1.
The capsular antigen was loaded into the polylactic-co-glycolic acid (PLGA) nanoparticles by W/O /W method (5). FT-IR and AFM were used to confirm capsule antigen loading and morphology of the nanoparticles, respectively (6,7). The fever after the vaccination was tested by limolus amoebocyte lysate assay (LAL test)(8). The investigation of the serum of mice by ELISA. Histopathological examination of lung, liver and spleen organs of vaccinated mice in four groups containing five female BALB/C mice (6-7 week-old) was studied after challenge with Klebsiella pneumoniae. Mice vaccinated with nanoparticles containing Klebsiella pneumoniae capsular antigen showed that the proposed vaccine has a high potential for long-term and stable protection against pure Klebsiella pneumoniae capsule antigen, activates (T Helper) lymphocytes T, and stimulates memory by stimulating T cells. Indicating the effectiveness of the vaccine, the results revealed that the vaccine could be recommended for animal studies with more samples or phase one clinical trial studies.

Keywords: Klebsiella pneumoniae, PLGA, nanoparticles, vaccine, capsular antigen.
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✅ Mice vaccinated with nanoparticles containing Klebsiella pneumoniae capsular antigen showed that the proposed vaccine has a high potential for long-term and stable protection against pure Klebsiella pneumoniae capsule antigen, activates (T Helper) lymphocytes T, and stimulates memory by stimulating T cells. Indicating the effectiveness of the vaccine, the results revealed that the vaccine could be recommended for animal studies with more samples or phase one clinical trial studies.


Type of Study: Letter to the Editor | Subject: Bionanotechnology
Received: 2021/01/9 | Accepted: 2021/01/30 | Published: 2021/11/17
References
1. Suster S, Moran CA. *Klebsiella* Pneumonia. In: Suster S, Moran CA, editors. Diagnostic Pathology: Thoracic (Second Edition) [Internet]. Second Edition. Philadelphia: Elsevier; 2017. p. 530-3. (Diagnostic Pathology). Available from: https://www.sciencedirect.com/science/article/pii/B9780323377157501134 [DOI:10.1016/B978-0-323-37715-7.50113-4]
2. Samanta I, Bandyopadhyay S. Chapter 14 - Klebsiella. In: Samanta I, Bandyopadhyay S, editors. Antimicrobial Resistance in Agriculture [Internet]. Academic Press; 2020. p. 153-69. Available from: https://www.sciencedirect.com/science/article/pii/B9780128157701000146 [DOI:10.1016/B978-0-12-815770-1.00014-6]
3. Elmowafy EM, Tiboni M, Soliman ME. Biocompatibility, biodegradation and biomedical applications of poly (lactic acid)/poly (lactic-co-glycolic acid) micro and nanoparticles. Journal of Pharmaceutical Investigation. 2019;49(4):347-80. [DOI:10.1007/s40005-019-00439-x]
4. Weintraub A. Immunology of bacterial polysaccharide antigens. Carbohydrate research. 2003;338(23):2539-47. [DOI:10.1016/j.carres.2003.07.008] [PMID]
5. Taha MA, Singh SR, Dennis VA. Biodegradable PLGA85/15 nanoparticles as a delivery vehicle for Chlamydia trachomatis recombinant MOMP-187 peptide. Nanotechnology. 2012;23(32):325101. [DOI:10.1088/0957-4484/23/32/325101] [PMID]
6. Shi Y, Yang H, Chu M, Niu X, Huo X, Gao Y, et al. Chapter 13 - Klebsiella. In: Amaresan N, Kumar MS, Annapurna K, Kumar K, Sankaranarayanan A, editors. Beneficial Microbes in Agro-Ecology [Internet]. Academic Press; 2020. p. 233-57. Available from: ttps://www.sciencedirect.com/science/article/pii/B9780128234143000137
7. Tosyali OA, Allahverdiyev A, Bagirova M, Abamor ES, Aydogdu M, Dinparvar S, et al. Nano-co-delivery of lipophosphoglycan with soluble and autoclaved leishmania antigens into PLGA nanoparticles: Evaluation of in vitro and in vivo immunostimulatory effects against visceral leishmaniasis. Materials Science and Engineering: C. 2021;120:111684. [DOI:10.1016/j.msec.2020.111684] [PMID]
8. Fischer S, Foerg C, Ellenberger S, Merkle HP, Gander B. One-step preparation of polyelectrolyte-coated PLGA microparticles and their functionalization with model ligands. Journal of Controlled Release. 2006;111(1-2):135-44. [DOI:10.1016/j.jconrel.2005.11.015] [PMID]
9. Naskar S, Das SK, Sharma S, Kuotsu K. A Review on Designing Poly (Lactic-co-glycolic Acid) Nanoparticles as Drug Delivery Systems. Pharm Nanotechnol. 2021;9(1):36-50. [DOI:10.2174/2211738508666201214103010] [PMID]
10. National Research Council, Guide for the Care and Use of Laboratory Animals: Eighth Edition, National Academies Press, Washington, DC, 2011.
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