Volume 32, Issue 152 (May & June 2024)                   J Adv Med Biomed Res 2024, 32(152): 226-234 | Back to browse issues page

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Khavasi N, Tavakolizadeh M, sardari S, ghassemifard L, reshadatifar M, darkhori M, et al . Evaluation of Antifungal and Antibacterial Effects of Capparis spinosa Root Extract on Streptococcus Mutans and Candida albicans. J Adv Med Biomed Res 2024; 32 (152) :226-234
URL: http://journal.zums.ac.ir/article-1-7357-en.html
1- Department of Persian Medicine, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran
2- Department of Pharmacognosy, School of Pharmacy, Zanjan University of Medical Sciences, Zanjan, Iran 3. Department of Pharmacognosy and Pharmaceutical Biotechnology, School of Pharmacy, Iran University of
3- Department of Oral and maxilla facial medicine, School of Dentistry, Zanjan University of Medical Sciences, Zanjan, Iran
4- Department of Oral and maxilla facial medicine, School of Dentistry, Zanjan University of Medical Sciences, Zanjan, Iran , m.mohebian@zums.ac.ir
5- Department of Microbiology, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran
6- Department of Parasitology and Mycology, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran
7- Social Determinants of Health Research Center, Zanjan University of Medical Sciences, Zanjan, Iran
Abstract:   (126 Views)
Background & Objective: Capparis spinosa is a plant belonging to the genus Capari which is important for medicinal purposes. It is also known as a multipurpose plant in Persian medicine. In this study, we searched for natural active ingredients in mouthwash for immunocompromised patients and examined the antifungal plus antibacterial properties of the hydroalcoholic extract of the root of the Capparis plant against Streptococcus mutans (S. mutans) PTCC 1683 and Candida albicans (C. albicans) ATCC-10231 was investigated.
  Materials & Methods: Ethanol extracts of plant roots were extracted by the percolation method. The diameter of the growth inhibition zone of C. albicans and S. mutans extracts was measured using the well plate method. Minimum inhibitory concentrations (MICs) were calculated for bacterial and fungal strains. In addition, the minimum sterilization concentration (MBC) and minimum sterilization concentration (MFC) of the extract were investigated using the broth microdilution method. The results were compared with an antibacterial and antifungal drug of 0.2% chlorhexidine nystatin.
 Results: The results for both pathogens revealed that the diameter of the growth inhibition zone increased with elevation of the extract concentration. The MIC and MBC of S. mutans extract were 62.5 and 62.5 mg/ml, compared to 0.39 and 0.39 mg/ml for chlorhexidine. The MIC and MFC of the plant against the fungus C. albicans were 7.812 and 31.25 mg/ml, compared to 0.25 and 1.25 μg/ml for nystatin.
 Conclusion:  The ethanol extract of the Caparis plant had an antifungal effect on C. albicans and an antibacterial effect on S. mutans. However, the inhibitory and lethal effects against bacterial and fungal strains were lower than those of nystatin and chlorhexidine.
 
Full-Text [PDF 767 kb]   (56 Downloads)    
Type of Study: Original Article | Subject: Medical Biology
Received: 2023/09/28 | Accepted: 2024/09/10 | Published: 2024/06/21

References
1. Krüger W, Vielreicher S, Kapitan M, Jacobsen ID, Niemiec MJ. Fungal-bacterial interactions in health and disease. Pathogens.2019;8(2):70. [DOI:10.3390/pathogens8020070]
2. Ray RR.Dental biofilm: Risks, diagnostics and management. Biotechnology Agri Biotechnol. 2022;43:102381. [DOI:10.1016/j.bcab.2022.102381]
3. Wu R, Cui G, Cao Y, Zhao W, Lin H. Streptococcus mutans membrane vesicles enhance Candida albicans pathogenicity and carbohydrate metabolism. Front Cell Infect Microbiol 2022;12:940602. [DOI:10.3389/fcimb.2022.940602]
4. de Faveri M, Pupio GC, Koo H, Bueno-Silva B, de Oliveira KM, Figueiredo LC, et al. The effect of Brazilian propolis type-3 against oral microbiota and volatile sulfur compounds in subjects with morning breath malodor. Clin Oral Investig. 2022;26(2):1531-1541. [DOI:10.1007/s00784-021-04125-x]
5. Ascar I. Biological effect of aqueous & alcoholic extracts of green tea leaves of some pathogenic bacteria in vitro. 2012(4):30-34.
6. Gavito-Higuera J, Mullins CB, Ramos-Duran L, Sandoval H, Akle N, Figueroa R. Sinonasal fungal infections and complications: a pictorial review. J Clin Imag Sci. 2016;6. [DOI:10.4103/2156-7514.184010]
7. Ramirez-Garcia A, Rementeria A, Aguirre-Urizar JM, Moragues MD, Antoran A, Pellon A, et al. Candida albicans and cancer: Can this yeast induce cancer development or progression? Crit Rev Microbiol. 2016;42(2):181-93.
8. Ponde NO, Lortal L, Ramage G, Naglik JR, Richardson J. Candida albicans biofilms and polymicrobial interactions.Crit Rev Microbiol. 2021;47(1):91-111. [DOI:10.1080/1040841X.2020.1843400]
9. Schulman J, Dimand RJ, Lee HC, Duenas GV, Bennett MV, Gould JB. Neonatal intensive care unit antibiotic use. Pediatrics. 2015;135(5):826-33. [DOI:10.1542/peds.2014-3409]
10. Pappas PG, Lionakis MS, Arendrup MC, Ostrosky-Zeichner L, Kullberg BJ. Invasive candidiasis. Nat Rev Dis Prim. 2018;4(1):1-20. [DOI:10.1038/nrdp.2018.26]
11. Kullberg BJ, Arendrup MC. Invasive candidiasis. New Eng J Med. 2015;373(15):1445-56. [DOI:10.1056/NEJMra1315399]
12. Daher MH, Muraih JK. Influence of Capparis spinosa crude extract on the amylase activity in vitro.Ann Roman Soc Cell Biol. 2021;25(2):2410-5.
13. Sardari S, Fallahiarezoudar F, Emadi F, Davati A, Faramarzi E, Fesharaki MG, et al. Capparis a multifunctional herb: evidence from the Iranian traditional medicine to modern medicine. Indo Am J Pharmaceut Sci. 2017;4(12).
14. Zhang H, Ma ZF. Phytochemical and pharmacological properties of Capparis spinosa as a medicinal plant. Nutrients. 2018;10(2):116. [DOI:10.3390/nu10020116]
15. Ghassemifard L, Sardari S, Safari H, Ramezanikhah H, Khavasi N. Assessment of the antioxidant activity of the extrcat, cold press, and n-hexane oils and in vitro cytotoxic effects of Capparis spinosa L. seed on SH-SY5Y cancer cell lines.J Med Plants. 2022;21(83):87-98. [DOI:10.52547/jmp.21.83.87]
16. Ashley Attard V. The medicinal plants in the Maltese islands and their ethnoveterinary aspects .2021.(Thesis).
17. Abu-Shama H. Effect of Caper (Capparis spinosa) extracts as a natural antimicrobial agent.J Food Dairy Sci Mansoura Univ .2019;10(7):209-16. [DOI:10.21608/jfds.2019.53494]
18. Mirza AS, Baig MT, Huma A, Ibrahim S, Shahid U, Jabeen A, et al. Antibacterial activity of methanol extract of Capparis decidua edgew (Forssk.) against Staphylococcus aureus, Bacillus cereus, Salmonella typhi, and Escherichia Coli.Pharmacophore. 2020;11(4).
19. Ghassemifard L, Sardari S, Safari H, Ramezanikhah H, Khavasi N. Assessment of the antioxidant activity of the extrcat, cold press, and n-hexane oils and in vitro cytotoxic effects of Capparis spinosa L. seed on SH-SY5Y cancer cell lines. J Med Plant. 2022;21(83):87-98. [DOI:10.52547/jmp.21.83.87]
20. Heydari S, Hosseini SH, Kamali K, Sardari S, Parsamanesh N, Ghassemifard L, et al. Capparis spinosa L. Reduces Cisplatin-induced acute liver injury in male rats: Pretreatment and single dose therapy. J Med Plant By-product. 2023;12(4):513-24.
21. Muhaidat R, Al-Qudah MA, Al-Shayeb A, Jacob JH, Al-Jaber HI, Hussein E, et al. Chemical profile and antibacterial activity of crude fractions and essential oils of Capparis ovata Desf. and Capparis spinosa L.(Capparaceae). Int J Integr Biol. 2013;14(1):39-47.
22. Shahrajabian MH, Sun W, Cheng Q. Plant of the Millennium, Caper (Capparis spinosa L.), chemical composition and medicinal uses. Bullet Nat Res Centre. 2021;45:1-9. [DOI:10.1186/s42269-021-00592-0]
23. Mahmoodpoor F, Hosseini SH, Ahmadian E, Ardalan M, Kamali K, Zununi Vahed S, et al. The histopathological evaluation of the effects of Capparis spinosa seed hydroalcoholic extract on cisplatin-induced-nephrotoxicity in rats. J Adv Med Biomed Res. 2023;31(144):73-9. [DOI:10.30699/jambs.31.144.73]
24. Al-Snafi AE. Therapeutic properties of medicinal plants: a review of their antibacterial activity (part 1). Int J Pharmacol Toxicol. 2015;6(3):137-58.
25. Benzidane N, Aichour R, Guettaf S, Laadel N, Khennouf S, Baghiani A, et al. Chemical investigation, the antibacterial and antifungal activity of different parts of Capparis spinosa extracts. J Drug Deliver Therap. 2020;10(5):118-25. [DOI:10.22270/jddt.v10i5.4388]
26. Gull T, Sultana B, Bhatti IA, Jamil A. Antibacterial potential of Capparis spinosa and Capparis decidua extracts. Int J Agri Biol. 2015;17(4). [DOI:10.17957/IJAB/14.0007]
27. Mazarei F, Jooyandeh H, Noshad M, Hojjati M. Polysaccharide of caper (Capparis spinosa L.) Leaf: Extraction optimization, antioxidant potential and antimicrobial activity. Int J Biol Macromolecule. 2017;95:224-31. [DOI:10.1016/j.ijbiomac.2016.11.049]
28. Mahasneh AM. Screening of some indigenous Qatari medicinal plants for antimicrobial activity. Phytother Res. 2002;16(8):751-3. [DOI:10.1002/ptr.1037]
29. Masadeh MM, Alkofahi AS, Alzoubi KH, Tumah HN, Bani-Hani K. Anti-Helicobactor pylori activity of some Jordanian medicinal plants. Pharmaceut Biol. 2014;52(5):566-9. [DOI:10.3109/13880209.2013.853811]
30. Ma ZF, Majid NA, Yamaoka Y, Lee YY. Food allergy and Helicobacter pylori infection: a systematic review. Front Microbiol. 2016;7:368. [DOI:10.3389/fmicb.2016.00368]
31. Boga C, Forlani L, Calienni R, Hindley T, Hochkoeppler A, Tozzi S, et al. On the antibacterial activity of roots of Capparis spinosa L. Nat Product Res. 2011;25(4):417-21. [DOI:10.1080/14786419.2010.487189]
32. Mahboubi M, Mahboubi A. Antimicrobial activity of as its usages in traditional medicine. Herba Polonica. 2014;60(1):39-48. [DOI:10.2478/hepo-2014-0004]

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