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Showing 4 results for Antibacterial

Mohmmad Mehdi ُsoltan Dallal, Zohreh Kalafi, Abdolaziz Rastegare Lari, Seyed Kazem Hosseini, Abbas Rahimi Foroushani, Zahra Deilami Khiabani, Farhad Nikkhahi, Siamak Heidarzadeh,
Volume 20, Issue 81 (9-2012)
Abstract

Background and Objective: Human amniotic membrane, which is the innermost layer of placenta, contains beta defensins and elafin, which both have antibacterial properties. These antibacterial activities depend on the conditions and environmental factors. The purpose of this study was to evaluate the effect of environmental factors on human amniotic membrane antibacterial properties against Klebsiella pneumonia (ATCC7881), Entrococcus faecalis (ATCC29212), Pseudomonas aeruginosa (ATCC27853), Salmonella enteric (BAA-708), and E. coli (ATCC25922) strains in vitro. Materials and Methods: The amniotic membrane samples were obtained from caesarean women in Imam Khomeini hospital. Participating women were all seronegative for HIV, HBV, HCV, and syphilis. The samples were cut into 1.5×1.5 cm pieces. The 0.5 McFarland bacterial suspensions were prepared and spread on Muller-Hinton agar medium, and a piece of membrane was placed in the centre of each plate. Samples were examined at different time intervals (24, 48, and 72 hr), temperatures (25, 33, 37°C) and pH (6.5, 7, and 7.5) as variables. Results: The results show that time and pH as variable parameters did not affect the antibacterial properties of the amniotic membrane. However, the change in temperature (25 and 33°C vs. 37°C) had a significant impact on P. aeruginosa. Conclusion: Antibacterial properties of the amniotic membrane seem resistant against environmental factors, except for especial cases, and this sustainability could expand its usage in clinical procedures and different conditions.


Edris Hoseinzadeh, Mohammad Yousef Alikhani, Mohammad Reza Samarghandy,
Volume 20, Issue 82 (7-2012)
Abstract

Background and Objective: This study aimed to determine the antibacterial activity of either zinc oxide or copper oxide nanoparticles alone or combined against Gram-positive and Gram-negative bacteria. Materials and Methods: In this experimental study Escherichia coli (E. coli ATCC 25922), Staphylococcus aureus (S.aureus ATCC 25923), Pesudomonas aueroginosa (P. aueroginosa ATCC 27853) and Staphylococcus Epidermidis (S. Epidermidis PTCC 1114) were used as test microorganisms. The Zinc and copper oxide nanoparticles were prepared commercially. The antibacterial activity of nanoparticles were studied using bacteriological tests such as minimum inhibitory concentration (MIC) and time kill study. Time kill studies were done using MIC nanoparticle concentration in mono and combined together mood for all the test microorganisms. The fractional inhibitory concentration (FIC) index used to define or to describe nanoparticle interactions at combined together mood of two nanoparticles. The ANOVA test (SPSS ver. 16) was used to compare the antibacterial activity of nanoparticles alone and combined. A P-value of ≤0.05 was considered significant. Results: The MIC value for E. coli and S.epidermidis against the combined nanoparticles had a lower concentration than mono metallic nanoparticles. By contrast, the MIC value for P.aeroginusa, and S. aureus against the combined mood of the nanoparticles had higher concentration. The fractional inhibitory concentration (FIC) index for E. coli, S.aureus, P. aueroginosa and S. epidermidis were 0.75, 9, 6, and 0.625, respectively. The results show that the Gram-negative bacteria seem to be more resistant to ZnO nanoparticles than the Gram-positive bacteria. Moreover, the antibacterial effect of nanoparticles in both mono and combined modes were time dependent (P<0.0001). Conclusion: The used commercial CuO/ZnO nanoparticles have great antibacterial potential against all of the strains, and the combination of zinc oxide and Copper oxide nanoparticles increases their bactericidal effect only for certain strains but not all.


Shohre Mohebbi, Maryam Hassan, Elaheh Gorji,
Volume 25, Issue 113 (8-2017)
Abstract

Background and Objective: Bacterial infections are an important and growing problem across the world. During the last few decades, the inappropriate consumption of antibiotics has led to increased antimicrobial resistance. A survey of literature demonstrates that isatin and indole rings are among the most favorable compounds with a potential for antimicrobial activity. Therefore in this study some novel isatin and indole derivatives coupled with hydantoin moiety were synthesized using a hybridization approach in order to evaluate the relationship between structure and antibacterial/antifungal activity.
Materials and Methods: The derivatives were prepared in good yields and their structures were confirmed using H-NMR, IR, and Mass Spectrometry. The antibacterial/antifungal activity of compounds was evaluated using the Broth Microdilution method on a series of gram positive and gram negative bacterial strains and Aspergillus niger.
Results: Among the tested compounds, the indole derivative #5 exhibited the highest activity against Pseudomonas aeruginosa with a MIC value of 31.25 µg/mL.
Conclusion[vog1] : These synthesized derivatives showed effective antibacterial activity mainly against Pseudomonas aeruginosa. On the other hand the tested compounds had appropriate spectrum effect ,because they showed antibacterial effect on both positive and negative strains.

 [vog1] the English conclusion s the hould be written as Persian conclusion.

Fatemeh Hosseinali Khani, Alireza Yazdinezhad, Somayeh Sadighian, Mehran Mohseni,
Volume 32, Issue 155 (10-2024)
Abstract

Background & Objective:  Antibiotic resistance has become a global problem. This study aimed to develop and evaluate nanoemulsions' capacity to improve Cuminum cyminum's antibacterial activity essential oil as an appropriate approach to prevent microbial resistance.
 Materials & Methods:  Gas chromatography-mass spectrometry was used to determine the main constituent of C. cyminum essential oil. FT-IR was used to investigate the functional groups of chemical composition. A nanoemulsion was generated using high-shear homogenization, followed by several physicochemical characterizations. The antibacterial activity of essential oil in both pure and nanoemulsion forms against important food-borne pathogens, and drug-resistant bacteria was determined by measuring the Minimum Inhibitory Concentration (MIC) and the Minimum Bactericidal Concentration (MBC).
Results:  Cumin aldehyde with 20.08%w/v is the main constituent of essential oil. The prepared nanoemulsions had an average size of 72 nm, particle distribution of 0.234, and zeta potential of 26 mV. Nanoemulsion formulations of essential oils are more effective compared to pure ones.   Klebsiella pneumonia and Pseudomonas aeruginosa have the highest and the lowest antimicrobial effects of essential oil among all studied bacteria, respectively. In contrast, in resistant samples, the major and minor effects were contributed to Methicillin-resistant Staphylococcus aureus and vancomycin-resistant Enterococcus, respectively.
Conclusion:  Cumin oil-nanoemulsion had an acceptable function against antibiotic-resistant bacteria. It is advisable to combine this formulation with synthetic antibiotics in order to reduce bacterial resistance and serve as a preservative in the pharmaceutical and food industries.
 

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