دوشنبه 9 تیر 1404
[Archive]
دوره 19، شماره 74 - ( 1-1390 )
جلد 19 شماره 74 صفحات 19-9 |
برگشت به فهرست نسخه ها
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Khorsandi L, Bahramzadeh S, Hashemitabar M, Kalantar Mahdavi S R. Metformin Effect on the Mouse Pancreatic Langerhans Islets Volume. J Adv Med Biomed Res 2011; 19 (74) :9-19
URL: http://journal.zums.ac.ir/article-1-1411-fa.html
URL: http://journal.zums.ac.ir/article-1-1411-fa.html
خرسندی لعیا، بهرام زاده سمیه، هاشمی تبار محمود، کلانتر مهدوی سید رشیدالدین. اثر متفورمین بر روی حجم جزایر لانگرهانس درپانکراس موش سوری. Journal of Advances in Medical and Biomedical Research. 1390; 19 (74) :9-19
1- دکترای بافت شناسی، استادیار مرکز تحقیقات سلولی و مولکولی دانشگاه علوم پزشکی جندی شاپور اهواز ، Layasadat@yahoo.com
2- کارشناس ارشد زیست شناسی گرایش سلولی تکوینی، مرکز تحقیقات سلولی و مولکولی دانشگاه علوم پزشکی جندی شاپور اهواز
3- دکترای بافت و جنین شناسی، دانشیار مرکز تحقیقات سلولی و مولکولی دانشگاه علوم پزشکی و خدمات بهداشتی درمانی جندی شاپور اهواز
4- کارشناس ارشد آناتومی، مربی دانشگاه علوم پزشکی جندی شاپور اهواز
2- کارشناس ارشد زیست شناسی گرایش سلولی تکوینی، مرکز تحقیقات سلولی و مولکولی دانشگاه علوم پزشکی جندی شاپور اهواز
3- دکترای بافت و جنین شناسی، دانشیار مرکز تحقیقات سلولی و مولکولی دانشگاه علوم پزشکی و خدمات بهداشتی درمانی جندی شاپور اهواز
4- کارشناس ارشد آناتومی، مربی دانشگاه علوم پزشکی جندی شاپور اهواز
چکیده: (171340 مشاهده)
Background and Objective: Metformin is a widely used medicine for treatment of type 2 diabetes. In this study, the effect of various doses of metformin on the mouse islets of langerhans volume was investigated.
Materials and methods: Twenty four C57BL/6 adult male mice weighting 30±5 gr were randomly divided into 4 groups. Normal saline was given to the control group (group 4) and the experimental groups (groups 1-3) received 75, 150 and 300 mg/kg metformin daily by intraperitoneal injection for seven days. One day after the last injection the mice were sacrificed by cervical dislocation and their pancreases were fixed in 10% formalin for histological studies. The volume of the islets of langerhans was estimated by using Cavalieri method.
Results: Volume of the islets of langerhans in doses of 75 and 150 mg/kg Metformin showed a non-significant difference in comparison to control group (P>0.05). 300 mg/kg metformin treated mice showed a significant increase in islets of langerhans volume compared to the control group (P<0.05).
Conclusion: Metformin increases in the islets of langerhans volume in a dose-dependent manner. Increasing effects of Metformin on the islets of langerhans volume may be due to proliferation or hypertrophy of beta cells.
نوع مطالعه: مقاله پژوهشی |
دریافت: 1389/12/17 | پذیرش: 1393/4/2 | انتشار: 1393/4/2
دریافت: 1389/12/17 | پذیرش: 1393/4/2 | انتشار: 1393/4/2
فهرست منابع
1. Guerra SD, Lupi R, Marselli L. Functional and molecular defects of pancreatic islets in human type 2 diabetes. Diabets. 2005; 54: 727-35. [DOI:10.2337/diabetes.54.3.727] [PMID]
2. Donath MY, Halban PA. Decreased beta-cell mass in diabetes: significance, mechanisms and therapeutic implications. Diabetologia. 2004; 47: 581-9. [DOI:10.1007/s00125-004-1336-4] [PMID]
3. Rhodes CJ. Type 2 Diabetes-a Matter of beta-Cell Life and Death? Science. 2005; 307: 380-4. [DOI:10.1126/science.1104345] [PMID]
4. Green BD, Irwin N, Duffy NA, Gault VA, O'Harte FPN, Flatt PR. Inhibition of dipeptidyl peptidase-IV activity by metformin enhances the antidiabetic effects of glucagon-like peptide-1. Euro J Pharmacol. 2006; 547: 192-9. [DOI:10.1016/j.ejphar.2006.07.043] [PMID]
5. Stumvoll M, Nurjhan N, Perriello G, Dailey G, Gerich JE. Metabolic effects of metformin in non-insulin-dependent diabetes mellitus. Engl J Med. 1995; 333: 550-4. [DOI:10.1056/NEJM199508313330903] [PMID]
6. Richardson H, Campbell SC. Effects of rosiglitazone and metformin on pancreatic beta cell gene expression. Diabetologia. 2006; 49:685-96. [DOI:10.1007/s00125-006-0155-1] [PMID]
7. Hull RL, Shen ZP. Long-term treatment with rosiglitazone and metformin reduces the extent of, but does not prevent, islet amyloid deposition in mice expressing the gene for human islet amyloid polypeptide. Diabetes. 2005; 54: 2235-44. [DOI:10.2337/diabetes.54.7.2235] [PMID]
8. Mannucci E, Ognibene A, Cremasco F, Bardini G, Mencucci A, Pierazzuoli E. Effect of Metformin on Glucagon-Like Peptide 1 (GLP-1) and Leptin Levels in Obese Nondiabetic Subjects. Diabets Care. 2001; 24: 489-94. [DOI:10.2337/diacare.24.3.489] [PMID]
9. Mojsov S, Heinrich G, Wilson IB, Ravazzola M, Orci L, Habener JF. Preproglucagon gene expression in pancreas and intestine diversifies at the level of post-translational processing. J Biol Chem. 1986; 261:11880-9.
10. Li Y, Hansotia T. Glucagon-like peptide-1 reseptor modulates beta cell apoptosis. Biol Chem. 2003; 278: 471-8. [DOI:10.1074/jbc.M209423200] [PMID]
11. Perfetti R, Zhou J, Doyle ME, Egan JM. Glucagon-Like Peptide-1 Induces Cell Proliferation and Pancreatic-Duodenum Homeobox-1 Expression and Increases Endocrine Cell Mass in the Pancreas of Old, Glucose-Intolerant Rat. Endocrinol. 2000; 141:4600-5. [DOI:10.1210/endo.141.12.7806] [PMID]
12. Howard CV, Reed MG. Unbiased stereology. Chester: Bios scientific. 1998; 39-45.
13. Gundersen HJ, Bendsten TF, Korbo L. Some new, simple and efficient stereological methods and theiruse in pathological research and diagnosis. Apmiss. 1988; 96: 391-4. [DOI:10.1111/j.1699-0463.1988.tb05320.x] [PMID]
14. Gundersen HJ, Bagger P, Bendtsen TF. The new stereological tools; Disector, fractionator; nucleator and point sampled intercepts and their use in pathological research and diagnosis. Apmiss. 1988; 96: 857-81. [DOI:10.1111/j.1699-0463.1988.tb00954.x] [PMID]
15. Corbett J, Serup P, Bonner-Weir S, Nielsen JH. B-Cell ontogeny: growth and death. Diabetologia. 1997; 40: B27-B32 [DOI:10.1007/BF03168183] [PMID]
16. Edvell A, Lindstrom P. Initiation of increased pancreatic islet growth in young normoglycemic mice. Endocrinol. 1999; 140: 778-83. [DOI:10.1210/endo.140.2.6514] [PMID]
17. Xu G, Stoffers DA, Habener JF, Bonner-Weir S. Exendin-4 stimulates both b-Cell replication and neogenesis, resulting in increased b-Cell mass and improved glucose tolerance in diabetic rats. Diabetes. 1999; 48: 2270-6. [DOI:10.2337/diabetes.48.12.2270] [PMID]
18. Greig N, Halloway HW, De Ore K, et al. Once daily injections of Exendin-4 to diabetic mice achieves long-term beneficial effects on blood glucose levels. Diabetologia. 1999; 42: 45-50. [DOI:10.1007/s001250051111] [PMID]
19. Tourrel C, Bailbe D, Meile MJ, Kergoat M, Portha B. Glucagon-Like Peptide-1 and Exendin-4 stimulate b-Cell neogenesis in streptozotocin-treated newborn rats resulting in persistently improved glucose homeostasis at adult age. Diabetes. 2001; 50: 1562-70. [DOI:10.2337/diabetes.50.7.1562] [PMID]
20. Couto MF, Minn AH, Pise-Masison CA, et al. Exenatide blocks JAK1-STAT1 in pancreatic beta cell. Metabolism. 2007; 56: 915-8. [DOI:10.1016/j.metabol.2007.02.004] [PMID]
21. Ling Z, Wu D, Zambre Y, et al. Glucagon-like peptide 1 receptor signaling influences topography of islet cells in mice. Virchows Arch. 2001; 438: 382-7. [DOI:10.1007/s004280000374] [PMID]
22. Guyton AC, Hall JE. Textbook of medical physiology. Mississippi: Saunders; 2005.
ارسال پیام به نویسنده مسئول
| بازنشر اطلاعات | |
 |
این مقاله تحت شرایط Creative Commons Attribution-NonCommercial 4.0 International License قابل بازنشر است. |
