دوره 19، شماره 76 - ( 3-1390 )                   جلد 19 شماره 76 صفحات 57-48 | برگشت به فهرست نسخه ها

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‌Vahidi A R, Khalili M A, Amini-rad O. Effects of Different Doses of Adenosine on Sperm Motility in Infertile Men. J Adv Med Biomed Res 2011; 19 (76) :48-57
URL: http://journal.zums.ac.ir/article-1-1570-fa.html
وحیدی علیرضا، خلیلی محمد علی، امینی‌راد امید. بررسی اثر دوزهای مختلف آدنوزین بر پارامتر تحرک اسپرم مردان نابارور. Journal of Advances in Medical and Biomedical Research. 1390; 19 (76) :48-57

URL: http://journal.zums.ac.ir/article-1-1570-fa.html

بررسی اثر دوزهای مختلف آدنوزین بر پارامتر تحرک اسپرم مردان نابارور
علیرضا وحیدی*1 ، محمد علی خلیلی2 ، امید امینی‌راد3
1- دکترای داروسازی، مربی دانشگاه علوم پزشکی شهید صدوقی یزد، مرکز تحقیقاتی و درمانی ناباروری یزد ، drztorabi@yahoo.com
2- دکترای تخصصی جنین‌شناسی، دانشیار دانشگاه علوم پزشکی شهید صدوقی یزد، مرکز تحقیقاتی و درمانی ناباروری و گروه بیولوژی علوم تشریح
3- دانشجوی پزشکی، دانشگاه علوم پزشکی شهید صدوقی یزد
چکیده:   (184781 مشاهده)

Background and objective: One of the causes of male infertility is the lack of proper mobility. One way to deal with this problem is to add adenosine to human sperms in the laboratory. Therefore, this study aimed to determine the effect of different doses of adenosine on sperm motility in the ejaculates of fertile and infertile individuals.
Materials and Methods: In this experimental laboratory study, 60 samples of semen fluid of infertile and fertile men who referred to Yazd Infertility Center were analyzed according to WHO guidelines. Sperm counts, morphology and motility (fast, slow and immotile) were studied. After using Swim-up technique, the washed samples were divided into 4 groups, containing doses of 0, 2, 5 and 10 mg adenosine. The sperm parameters were evaluated after the addition of adenosine. Data were analyzed statistically using paired T-test and independent T-test.
Results: The initial assessment indicated significant differences in sperm parameters, except for slow motility between the two groups. In fertile group, dose of 5 mg adenosine increased sperm count, and rapid motility, but lowered the rates of sperm immotility at doses of 5 and 10 mg. In the infertile group, sperm count improved at dose of 5 mg adenosine, but it showed no effect on slow sperm motility. 5 mg adenosine also improved rapid sperm motility, but dose of 10 mg had no effect. Both 5 and 10 mg adenosine significantly lowered immotile sperm rates.
Conclusion: Based on the results of this study, it can be concluded that addition of 5 mg adenosine to washed sperms can improve sperm motility of infertile men in vitro.

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نوع مطالعه: مقاله پژوهشی |
دریافت: 1390/6/2 | پذیرش: 1393/4/1 | انتشار: 1393/4/1
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