دوره 24، شماره 105 - ( 4-1395 )                   جلد 24 شماره 105 صفحات 77-67 | برگشت به فهرست نسخه ها

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Sharifi A, Mohammadzadeh A, Mahmoodi P, Sasanian N. Inhibitory Effect of Thymus daenensis Essential Oil on Staphylococcus aureus NorA Efflux Pump. J Adv Med Biomed Res 2016; 24 (105) :67-77
URL: http://journal.zums.ac.ir/article-1-3584-fa.html
شریفی آرام، محمدزاده عبدالمجید، محمودی پژمان، ساسانیان نگار. بررسی اثر مهاری اسانس گیاه آویشن بر روی افلوکس پمپ NorA استافیلوکوکوس اورئوس. Journal of Advances in Medical and Biomedical Research. 1395; 24 (105) :67-77

URL: http://journal.zums.ac.ir/article-1-3584-fa.html


1- دانشگاه بوعلی سینا همدان
2- دانشگاه بوعلی سینا همدان ، Mohammadzadeh4@hotmail.com
چکیده:   (154667 مشاهده)

Background and Objective: Antibiotic resistant phenotypes of bacteria have been shown to be related to efflux pumps. Research for finding compounds with an ability to inhibit these pumps seems worthwhile due to their ability to increase bacterial sensitivity to antibiotics or return sensitivity to resistant strains. The aim of this study was to evaluate inhibitory effect of Thymus daenensis essential oil (EO) on NorA efflux pump of staphylococcus aureus.

Materials and Methods: Thymus daenensis essential oil was extracted from dried thyme leaves by hydrodistillation method and then the effect of the essential oil on bacterial efflux pump was evaluated using ethidium bromide-agar cartwheel method with respect to bacterial efflux capacity of ethidium bromide. Minimum inhibitory concentration (MIC) of Thymus daenensis essential oil was measured using microdilution test followed by the evaluation of the effect of sub-MIC concentration of essential oil on fluoroquinolone antibiotics. The efflux level of ethidium bromide, ciprofloxacin and norfloxacin (substances transmitted by NorA) was considered as the NorA efflux pump activity.

Results: The cartwheel method showed that Thymus daenensis essential oil could reduce the bacterial efflux capacity of ethidium bromide. The results of checkerboard test indicated that the interactions between sub-MIC concentration of the essential oil and ethidium bromide, ciprofloxacin and norfloxacin were totally synergistic.

Conclusion: Synergistic effect of Thymus daenensis essential oil with the other components demonstrated the potential of Thymus daenensis to enhance the antimicrobial activity of fluoroquinolone antibiotics against staphylococcus aureus. As these antibiotics are effluxed by NorA pump, this increased sensitivity to antibiotics is possibly due to NorA efflux pump inhibitors.

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نوع مطالعه: کارآزمایی بالینی |
دریافت: 1394/12/26 | پذیرش: 1394/12/26 | انتشار: 1394/12/26

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