دوره 24، شماره 107 - ( 6-1395 )                   جلد 24 شماره 107 صفحات 83-71 | برگشت به فهرست نسخه ها

XML English Abstract Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Dehghanpour M, Baghcheh Saraee H, Mosavari N, Mazloom Zadeh S. Isolation of Mycobacterium Bovis from Tuberculosis Patients in Zanjan Province (2013). J Adv Med Biomed Res 2016; 24 (107) :71-83
URL: http://journal.zums.ac.ir/article-1-3774-fa.html
دهقانپور مهدی، باغچه سرایی حمید، مصوری نادر، مظلوم زاده سعیده. جداسازی مایکوباکتریوم بویس از مسلولین استان زنجان در سال 1392. Journal of Advances in Medical and Biomedical Research. 1395; 24 (107) :71-83

URL: http://journal.zums.ac.ir/article-1-3774-fa.html


1- دانشگاه علوم پزشکی زنجان ، m.dehghanpour1983@yahoo.com
2- دانشگاه علوم پزشکی زنجان
3- سرم سازی رازی کرج
چکیده:   (153487 مشاهده)

Background and Objective: Tuberculosis (TB) is amongst the common infectious diseases in the world so that the World Health Organization (WHO) has declared it as a global emergency as well as an extremely contagious disease. Although the cause of the disease and its therapy are well known, TB is considered as a global dilemma. The present study was set to isolate and identify Mycobacterium Tuberculosis Complex by PCR-RD typing from TB patients in Zanjan province and to verify cases of zoonotic tuberculosis.

Materials and Methods: In this study, 27 positive samples were collected from center of pulmonary diseases and tuberculosis in Zanjan Province during 2013 and were grown on Lowenstein–Jensen medium (LJP and LJG). DNA was extracted from the bacterial isolates by Van Solengen method and at genus level identification was done by PCR-16SrRNA. To confirm the mycobacterium tuberculosis complex, PCR-IS6110 was used. Identification of species was performed by PCR-RD typing (RD1, RD4, RD9 and RD12).

Results: A total of 27 isolates were obtained which were identified as genus Mycobacterium belonging to Mycobacterium tuberculosis complex. Twenty five of the isolates were Mycobacterium tuberculosis while 2 isolates were identified as M.bovis.

Conclusion: The results of the study indicates that M.bovis species is present among the human TB cases in Zanjan province, which indicates the zoonotic potential of the species and the possible transmission of the species from animals to humans. These results might be alarming owing to the spread of the bovine strains among the human subjects. Thus, it is essential that during research on suspected human TB cases, the zoonotic species capable of transmission from animal to human be considered.

متن کامل [PDF 286 kb]   (159808 دریافت)    
نوع مطالعه: کارآزمایی بالینی |
دریافت: 1395/3/31 | پذیرش: 1395/3/31 | انتشار: 1395/3/31

ارسال پیام به نویسنده مسئول


بازنشر اطلاعات
Creative Commons License این مقاله تحت شرایط Creative Commons Attribution-NonCommercial 4.0 International License قابل بازنشر است.

کلیه حقوق این وب سایت متعلق به Journal of Advances in Medical and Biomedical Research می باشد.

طراحی و برنامه نویسی : یکتاوب افزار شرق

© 2025 CC BY-NC 4.0 | Journal of Advances in Medical and Biomedical Research

Designed & Developed by : Yektaweb