دوره 24، شماره 107 - ( 6-1395 )                   جلد 24 شماره 107 صفحات 118-107 | برگشت به فهرست نسخه ها

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Sohrabi R, Zeighami H. Determination of Phylogenetic Groups and Antibiotic Resistance in Uropathogenic and Commensal Escherichia Coli Isolated from Patients in Zanjan City. J Adv Med Biomed Res 2016; 24 (107) :107-118
URL: http://journal.zums.ac.ir/article-1-3778-fa.html
سهرابی رویا، ضیغمی حبیب. تعیین گروه‌های فیلوژنتیک و مقاومت آنتی‌بیوتیکی در ایزوله‌های اشریشیاکلی اوروپاتوژنیک و کامنسال جدا شده از شهر زنجان. Journal of Advances in Medical and Biomedical Research. 1395; 24 (107) :107-118

URL: http://journal.zums.ac.ir/article-1-3778-fa.html


1- دانشگاه آزاد اسلامی واحد زنجان
2- دانشگاه علوم پزشکی زنجان ، zeighami@zums.ac.ir
چکیده:   (155446 مشاهده)

Background and Objective: Escherichia coli strains are the major cause of urinary tract infections which belong to the large group of extra-intestinal pathogenic E. coli. They fall into four main phylogenetic groups: A, B1, B2 and D. The aim of the present study was to determine phylogenetic groups in uropathogenic and commensal Escherichia coli isolated. Also, due to increase in the rate of antibiotic usage and subsequent drug resistance, this study evaluated the antimicrobial resistance pattern of E. coli isolated from patients with UTI.

Materials and Methods: In this cross-sectional study, 137 uropathogenic Escherichia coli isolates were collected from the clinical specimens of Zanjan hospitals and 50 isolates were collected from healthy adults. After verifying isolates via biochemical methods and extraction of total DNA, Multiplex PCR was done by specific primers for phylogenetic grouping. The antibiotic susceptibility test (disk diffusion method) was done according to CLSI advice against 13 antibiotics.

Results: In this study, the highest rates of resistance to antibiotics in UTI isolates were seen against ampicillin (74.5%) and aztreonam (59.1%). Also, the lowest rates of resistance were reported against imipenem (1.5%) and amikacin (10.9%).The distribution of UPEC phy logenetic groups typing marked the highest prevalence in group B2 (67.15 %) , and then in group D (21.17%) and the lowest prevalence in group A (11.68%). Phy logenetic B1 was not observed in uropathogenic isolates. Among the commensal isolates, 52% belonged to phylogenetic group D, 24% to B2, 14% to A, and10% to B1.

Conclusion: The results indicate the higher prevalence of B2 and D phylogenetic groups in commensal and uropathogenic Escherichia coli strains in Zanjan.

متن کامل [PDF 214 kb]   (159808 دریافت)    
نوع مطالعه: کارآزمایی بالینی |
دریافت: 1395/3/31 | پذیرش: 1395/3/31 | انتشار: 1395/3/31

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