دوره 26، شماره 115 - ( 3-1397 )                   جلد 26 شماره 115 صفحات 68-53 | برگشت به فهرست نسخه ها

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AkBari H, Zeighami H, Mohseni M. Evaluation of Direct Mutagenesis in some of Natural Food Colors Using Ames Test. J Adv Med Biomed Res 2018; 26 (115) :53-68
URL: http://journal.zums.ac.ir/article-1-5050-fa.html
اکبری هاله، ضیغمی حبیب، محسنی مهران. ارزیابی وجود جهش‌زایی مستقیم در چند نوع از رنگ‌های طبیعی خوراکی مورد استفاده در مواد غذایی با آزمون Ames. Journal of Advances in Medical and Biomedical Research. 1397; 26 (115) :53-68

URL: http://journal.zums.ac.ir/article-1-5050-fa.html

ارزیابی وجود جهش‌زایی مستقیم در چند نوع از رنگ‌های طبیعی خوراکی مورد استفاده در مواد غذایی با آزمون Ames
هاله اکبری1 ، حبیب ضیغمی2 ، مهران محسنی*3
1- کارشناسی ارشد بهداشت و ایمنی مواد غذایی، دانشکده بهداشت و پیراپزشکی، دانشگاه علوم پزشکی زنجان، زنجان
2- دکترای تخصصی باکتری شناسی، استادیار گروه میکروب شناسی، دانشکده پزشکی، دانشگاه علوم پزشکی زنجان، زنجان
3- دکترای تخصصی مواد خوراکی، دانشیار گروه کنترل غذا و دارو، دانشکده داروسازی، دانشگاه علوم پزشکی زنجان، زنجان ، mohsenim@zums.ac.ir
چکیده:   (149737 مشاهده)

Background and Objective: Nowadays, colors are widely used in various food industries, including sweets, confectionery, ice cream, beverages, and so on. Colors can have asthma-like complications, hyperactivity in children and cancers. Therefore, the aim of this study was to evaluate the direct mutagenic effect of several types of natural colors via Ames method.
Materials and Methods: After preparation of 6 natural colors from three different brands, their direct mutagenesis was investigated by Ames test. In this test, the strains of Salmonella typhimurium TA98 and TA100, carrying the selective mutation in His-Hiscoprotein Histidine, were cultured on glucose culture medium at least in the presence of color samples, and only the bacteria that had His + mutation were colonized.
Results: Direct mutagenicity of the food colors was measured by counting colonies in the plates containing edible color and comparing it with negative controls. The results showed that the number of referent colonies in plates containing cherry red and golden yellow for company B for both strains and the dark brown color of company B and C, and orange color for TA100 and TA98 strains in some concentrations were more than twice comparing the negative control plates and had direct mutagenic effects. The dark green and light green colors of the A, B, C, and golden yellow for companies A, C and cherry red and orange for companies C did not show direct mutagenic activity.
Conclusion: Based on these results, some of the studied colors, despite being declared natural, showed direct mutagenic activity.

متن کامل [PDF 4527 kb]   (156157 دریافت)    
نوع مطالعه: کارآزمایی بالینی |
دریافت: 1396/11/16 | پذیرش: 1396/11/16 | انتشار: 1396/11/16
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