جمعه 26 اردیبهشت 1404
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دوره 26، شماره 119 - ( 8-1397 )
جلد 26 شماره 119 صفحات 33-24 |
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Rezazadeh K, Chehelcheraghi F, Anbari K. The Effect of Bone Marrow Derived Mesenchymal Stem Cells on the Survival of Random Skin Flap on Sterptozotocin-Induced Diabetic Rats. J Adv Med Biomed Res 2018; 26 (119) :24-33
URL: http://journal.zums.ac.ir/article-1-5223-fa.html
URL: http://journal.zums.ac.ir/article-1-5223-fa.html
رضازاده خدیجه، چلچراغی فرزانه، عنبری خاطره. تأثیر سلول‌های بنیادی مزانشیمی مشتق از مغز استخوان بر بقای فلپ تصادفی پوست در موش‌های دیابتی شده با استرپتوزوتوسین. Journal of Advances in Medical and Biomedical Research. 1397; 26 (119) :24-33
1- ، chehelcheraghi.farzaneh@lums.ac.ir
چکیده: (148535 مشاهده)
Background & Objective: Wound dressing and healing in diabetic patients is encountered with many problems. This study aims to investigate the effect of bone marrow derived mesenchymal stem cells (BM-MSCs) on the survival of random skin flap (RSF) on Streptozotocin-induced diabetic rats (STZ) using an optical microscope.
Materials & Methods: In this study, 60 male Albino Wistar rats were used (average weight 250-300 gr). The rats were divided into six groups: 1). Health-Non (HN), 2). Health- Cells (HC), 3). Health –Sham (HS), 4). Diabetic-Non (DN) that were became diabetic by injecting STZ 70 mg/kg intraperitoneally), 5). Diabetic-Sham (DS), 6). And Diabetic-Cell (DC). In all groups, the day of surgery was considered as the zero day, on the back area of animal, the flap was created with a size of 8 × 3 cm and the BM-MSCs were performed. The sampling was performed on day 7 after surgery from the region where Transitional Zone (TZ) necrosis was initiated.
Results: BM-MSCs increased the number of blood vessels (P=0.009) and the histology parameters (wound demarcation P=0.0001, granulation tissue P=0.0001) significantly compared to the control group. But this increase was not significant in the area of the survival region.
Conclusion: It was concluded that after treatment with BM-MSCs, the wound healing process in both non-diabetic and diabetic groups was increased in accordance with histological characteristics.
Materials & Methods: In this study, 60 male Albino Wistar rats were used (average weight 250-300 gr). The rats were divided into six groups: 1). Health-Non (HN), 2). Health- Cells (HC), 3). Health –Sham (HS), 4). Diabetic-Non (DN) that were became diabetic by injecting STZ 70 mg/kg intraperitoneally), 5). Diabetic-Sham (DS), 6). And Diabetic-Cell (DC). In all groups, the day of surgery was considered as the zero day, on the back area of animal, the flap was created with a size of 8 × 3 cm and the BM-MSCs were performed. The sampling was performed on day 7 after surgery from the region where Transitional Zone (TZ) necrosis was initiated.
Results: BM-MSCs increased the number of blood vessels (P=0.009) and the histology parameters (wound demarcation P=0.0001, granulation tissue P=0.0001) significantly compared to the control group. But this increase was not significant in the area of the survival region.
Conclusion: It was concluded that after treatment with BM-MSCs, the wound healing process in both non-diabetic and diabetic groups was increased in accordance with histological characteristics.
نوع مطالعه: مقاله پژوهشی |
موضوع مقاله:
Medical Laboratory and Animal Investigation
دریافت: 1397/3/23 | پذیرش: 1398/5/27 | انتشار: 1397/7/9
دریافت: 1397/3/23 | پذیرش: 1398/5/27 | انتشار: 1397/7/9
فهرست منابع
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