پنجشنبه 14 تیر 1403
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دوره 31، شماره 144 - ( 10-1401 )
جلد 31 شماره 144 صفحات 92-86 |
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Azadfar P, Noormohammadi Z, Noroozian M, Eidi A, Mortazavi P. Effect of Memantine on Expression of NAT-Rad18, Rad18 and Sorl1 Genes in Rat Model of Alzheimer's Disease. J Adv Med Biomed Res 2023; 31 (144) :86-92
URL: http://journal.zums.ac.ir/article-1-6771-fa.html
URL: http://journal.zums.ac.ir/article-1-6771-fa.html
Effect of Memantine on Expression of NAT-Rad18, Rad18 and Sorl1 Genes in Rat Model of Alzheimer's Disease. Journal of Advances in Medical and Biomedical Research. 1401; 31 (144) :86-92
چکیده: (10990 مشاهده)
Background and Objective: Dysregulation of long-term expression of non-coding RNAs (lncRNAs) has a potential role in progressive brain disorders such as Alzheimer's disease. This study aimed to analyze the apoptosis and expression of 51A and NAT-Rad18 lncRNAs and their target genes in brain tissue and peripheral blood mononuclear cells (PBMCs) of the rat model of AD, before and after memantine treatment.
Materials and Methods: Twenty-eight male Wistar rats were divided into four groups: 1. Normal control (n = 4), 2. Sham-operated (n = 4), 3. Alzheimer's control (n = 10), and 4. The experimental group (n = 10) was treated with memantine. The qPCR and TUNEL tests were used to detect the lncRNAs expression and apoptosis.
Results: Sorl1 gene was reduced in brain tissue of Alzheimer’s control (p = 0.016) and PBMCs of Alzheimer's control and experimental groups (p = 0.002 and p = 0.001 respectively). The expression of NAT-Rad18 and Rad18 genes increased in brain tissue of Alzheimer's control group (p = 0.002 and p = 0.04 respectively) while reduced in PBMCs of Alzheimer's control and experimental groups (p = 0.005 and p = 0.045 for NAT-Rad18, p = 0.01 and p = 0.006 for Rad18).
Conclusion: ROC curve analysis showed 100% sensitivity and 85.7% specificity for the Sorl1 gene with 0.911 under the curve area and 100% sensitivity and 100% specificity for NAT-Rad18 and Rad18, separately with one under the curve area. Decreased expression in Sorl1, NAT-Rad18, and Rad18 genes can be used as blood biomarkers for diagnosis independently. However, studies on Alzheimer's patients are needed.
نوع مطالعه: مقاله پژوهشی |
موضوع مقاله:
Medical Biology
دریافت: 1400/8/5 | پذیرش: 1401/5/11 | انتشار: 1401/9/21
دریافت: 1400/8/5 | پذیرش: 1401/5/11 | انتشار: 1401/9/21
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