دوره 26، شماره 114 - ( 1-1397 )                   جلد 26 شماره 114 صفحات 97-84 | برگشت به فهرست نسخه ها

XML English Abstract Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Khazaei M, Mirazi N. The Effect of Agrimonia eupatoria Leaf Hydroalcoholic Extract on Carbon Tetrachloride Induced Liver Toxicity in Male Rats. J Adv Med Biomed Res 2018; 26 (114) :84-97
URL: http://journal.zums.ac.ir/article-1-4964-fa.html
خزائی مریم، میرازی ناصر. اثر عصاره‌ی هیدروالکلی برگ گیاه غافث (Agrimonia eupatoria L.) بر سمیت کبدی در موش‌های صحرایی نر القا شده با تتراکلرید کربن. Journal of Advances in Medical and Biomedical Research. 1397; 26 (114) :84-97

URL: http://journal.zums.ac.ir/article-1-4964-fa.html

اثر عصاره‌ی هیدروالکلی برگ گیاه غافث (Agrimonia eupatoria L.) بر سمیت کبدی در موش‌های صحرایی نر القا شده با تتراکلرید کربن
مریم خزائی 1، ناصر میرازی2
1- دانشکده‌ی علوم پایه، دانشگاه بوعلی سینا، همدان ، maryam.khazaei133@yahoo.com
2- دانشکده‌ی علوم پایه، دانشگاه بوعلی سینا، همدان
چکیده:   (155578 مشاهده)

Background and Objective: Carbon tetrachloride (CCl4) is a toxic agent leading to hepatotoxicity. The aim of this study was to investigate the effect of Agrimonia eupatoria leaf hydroalcoholic extract (AEE) on carbon tetrachloride induced rat liver.
Materials and Methods: In this study, 42 male rats were randomly allocated to 6 groups: the control group receiving normal saline, 2ml/kg, ip and the sham group receiving olive oil, 2ml/kg, ip, the CCl4   induced group receiving carbon tetrachloride/olive oil  (1:1), 2ml/kg, single dose, ip. The treated groups (1, 2 and 3) were induced by carbon tetrachloride 1:1 with olive oil, 2ml/kg, single dose, ip and after two hours underwent treatment by 200 mg/kg, 400 mg/kg and 800 mg/kg AEE/day for 96 hrs, ip. Then, the blood samples were collected from their heart directly and subsequently serum levels of the liver enzymes were measured. The microscopic studies of liver tissue were performed. The data were analyzed with ANOVA and Tukey tests.
Results: The results of this study showed that the serum levels of liver enzymes significantly increased in the group receiving CCl4 compared to the control group. Meanwhile, treatment by AEE significantly decreased the serum levels of liver enzymes in treated groups compared to the group receiving CCl4 (P<0.001). Histological study revealed necrosis and inflammation in the liver tissue in CCl4 group and reduction in necrotic cells and inflammation in AEE treated group.
Conclusion: Carbon tetrachloride can bring about necrosis and inflammation effects on the liver tissue. AEE can decrease toxic effects of carbon tetrachloride in the liver tissue by reducing the serum levels of liver enzymes.  

متن کامل [PDF 3830 kb]   (157566 دریافت)    
نوع مطالعه: کارآزمایی بالینی |
دریافت: 1396/9/26 | پذیرش: 1396/9/26 | انتشار: 1396/9/26
فهرست منابع
1. Jamali R, Jamali A. Non-alcoholic fatty liver disease. Feyz. 2010; 14: 169-81.
2. Terohid SF, Mirazi N, Sarihi A. Study of hepatoprotective effect of Malva neglecta L. hydroethanolic leaf extract in male rat induced with carbon tetrachloride. J Cell & Tissue. 2015; 6: 31-42.
3. Taghikhani A, Ansarisamani R,Afrogh H, et al. The hepatotoxic and nephrotoxic effects of Stachys lavandulifolia Vahl in rat. J Mazandaran Univ Med Sci. 2012; 22:84-90.
4. Ahmadi Zadeh M, Amir Gholami F. Effects of cimetidine on CCl4-induced hepatotoxicity. Jundishapur Sci Med J. 2010; 2: 1-8.
5. Gangarapu V, Gujjala S, Korivi R, Pala I. Combined effect of curcumin and vitamin E against CCl4 induced liver injury in rats. Am J Life Sci. 2013; 1: 117-124. [DOI:10.11648/j.ajls.20130103.17]
6. Onuoha SC, Chinaka NC. Carbon tetrachloride induced renal toxicity and the effect of aqueous extract of Gongronema latifolium in Wistar albino rats. Drug discovery. 2013; 4: 15-16.
7. Mochizuki M, Shimizu S, Urasoko Y, et al. Carbon tetrachloride-induced hepatotoxicity in pregnant and lactating rats. J Toxicol Sci. 2009; 34: 175-181. [DOI:10.2131/jts.34.175] [PMID]
8. Kamalakkannan N, Rukkumani R, Aruna K, Varma PS, Viswanathan P, Menon VP. Protective effect of N-acetyl cysteine in carbon tetrachloride-induced hepatotoxicity in rats. Iranian J Pharmacol Ther. 2005; 4: 118-123.
9. Kuriakose GC, Kurup GM. Antioxidant activity of Aulosira fertilisima on CCl4 induced hepatotoxicity in rats. Indian J Exp Biol. 2008; 46: 52-59.
10. Roozbehi S, Razmi N, Akbartabar Touri M, Sadeghi H. Hepatoprotective effects of Bupleurum exalatum extracts against carbon tetrachloride induced liver injury in rats. Armaghane-danesh. 2015; 19: 1069-1081.
11. Mirazii N, Gholami M. Study of protective effect of Avicennia marina leaf hydroalchoholic extract on bone marrow tissue in male rate induced with CCl4. Arak Univ Med J. 2016; 19: 88-98.
12. Ad'hiah AH, Al-Bederi ONH, Al-Sammarrae KW. Cytotoxic effects of Agrimonia eupatoria L. against cancer cell lines in vitro. J Assoc Arab Univ Basic Appl Sci. 2013; 14: 87-92. [DOI:10.1016/j.jaubas.2013.01.003]
13. Rechinger KH. Flora Iranica. Akademische Druck-u Verlagsanstalt, Graz. 1969; 148-152.
14. Ivanova D, Tasinov O, Vankova D,Kiselova-Kaneva Y. Antioxidative potentialof Agrimonia eupatoria L. Sci Technol. 2011; 1: 20-24.
15. Correia HS, Batista MT, Dinis TC. The activity of an extract and fraction of Agrimonia eupatoria L. against reactive species. Biofactors. 2007; 29: 91-104. [DOI:10.1002/biof.552029209] [PMID]
16. Mirjana Z, Muruzovic KG, Mladenovic OD, Stefanovic SM, Vasic LR. Extracts of Agrimonia eupatoria L. as sources of biologically active compounds and evaluation of their antioxidant, antimicrobial, and antibiofilm activities. J Food Drug Anal. 2016; 24: 539-47. [DOI:10.1016/j.jfda.2016.02.007] [PMID]
17. Najafpour Navaei M, Golipour M, Parsa E. The effects of densities and planting dates on seed yield of Agrimonia eupatoria L. Iran J Med Aromat Plant. 2008; 24: 198-206.
18. Mirazi N, Movassagh SN, Rafieian-Kopaei M. The protective effect of hydro-alcoholic extract of mangrove (Avicennia marina L.) leaves on kidney injury induced by carbon tetrachloride in male rats. J Nephropathol. 2016; 5: 118-122. [DOI:10.15171/jnp.2016.22] [PMID] [PMCID]
19. Masjedi MS, Keyhanfar M, Behbahani M. In vitro evaluation of methanol extract of Agrimonia eupatoria on PBMC and 12 Pathogenic Bacteria. J Med Plant. 2017; 1: 167-73.
20. Ebrahimi S, Sadeghi H, Pourmahmoudi A, Askariyan SH, Askari S. Protective effect of Zizphus Vulgaris extract, on liver toxicity in laboratory rats. Yasuj Univ Med Sci. 2010; 16: 172-80.
21. Yoon SJ, Koh EJ, Kim CS, et al. Agrimonia eupatoria protects against chronic ethanol-induced liver injury in rats. Food Chem Toxicol. 2012; 50: 2335-41. [DOI:10.1016/j.fct.2012.04.005] [PMID]
22. Erasto P, Grierson DS, Afolayan AJ. Evaluation of antioxidant activity and the fatty acid profile of the leaves of Vernoniaamygdalina growing in South Africa. Food Chem. 2007; 104: 636-642. [DOI:10.1016/j.foodchem.2006.12.013]
23. Kaledaite R, Bernatoniene J, Majiene D, et al. Investigation of antiradical activity of Salvia officinalis L, Urticadioica L, Thymus vulgaris L. extracts as potential candidates for a complex therapeutic preparation. J Med Plant Res. 2011; 5: 6090-96.
24. Rahmani AH, Goudarzi M, Rashidi Nooshabadi MR, Houshmand Gh, Khadem Haghighian H. Protective effect of red Lentil (Lens Culinaris) extract against carbon tetrachloride-induced hepatotoxicity in mice. J Babol Univ Med Sci. 2014; 16: 49-55.
25. Rice-Evans CA, Miller NJ, Paganga G. Structure-antioxidant activity relationships of flavonoids and phenolic acids. Free Rad Biol Med. 1996; 20: 933-56. [DOI:10.1016/0891-5849(95)02227-9]
ارسال پیام به نویسنده مسئول

بازنشر اطلاعات
Creative Commons License این مقاله تحت شرایط Creative Commons Attribution-NonCommercial 4.0 International License قابل بازنشر است.

کلیه حقوق این وب سایت متعلق به Journal of Advances in Medical and Biomedical Research می باشد.

طراحی و برنامه نویسی : یکتاوب افزار شرق

© 2024 CC BY-NC 4.0 | Journal of Advances in Medical and Biomedical Research

Designed & Developed by : Yektaweb