Volume 30, Issue 139 (March & April 2022)                   J Adv Med Biomed Res 2022, 30(139): 154-161 | Back to browse issues page

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Esmaeil Zadeh M R, Sharifi Yazdi M K, Rajabi Z, Amin Harati F, Nikkhahi F, Sharifi Yazdi S, et al . Evaluation of Specific Bacteriophage Against Salmonella infantis and Its Antibacterial Effects Compared to Ciprofloxacin in In Vitro Conditions. J Adv Med Biomed Res 2022; 30 (139) :154-161
URL: http://journal.zums.ac.ir/article-1-6281-en.html
Evaluation of Specific Bacteriophage Against Salmonella infantis and Its Antibacterial Effects Compared to Ciprofloxacin in In Vitro Conditions
Mohammad Reza Esmaeil Zadeh1 , Mohammad Kazem Sharifi Yazdi2 , Zahra Rajabi3 , Farzaneh Amin Harati1 , Farhad Nikkhahi1 , Sara Sharifi Yazdi4 , Gholamreza Hassanpour5 , Alireza Monadi Sefidan6 , Mohammad Mehdi Soltan Dallal * 7
1- Dept. of Pathobiology, Faculty of Public Health, Tehran University of Medical Sciences, Tehran, Iran
2- Zoonosis Research Center, Tehran University of Medical Sciences, Tehran, Iran
3- Food Microbiology Research Center, Tehran University of Medical Sciences, Tehran, Iran
4- Medical Student, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran
5- Center for Research of Endemic Parasites of Iran, Tehran University of Medical Sciences, Tehran, Iran
6- Dept. of Medical Laboratory Sciences, Faculty of Allied Medical Sciences, Tehran University of Medical Sciences, Tehran, Iran
7- Dept. of Pathobiology, Faculty of Public Health, Tehran University of Medical Sciences, Tehran, Iran , msoltandallal@gmail.com
Abstract:   (92687 Views)

Background and Objective: Phage therapy could be used as an alternative method to antibiotic treatments. The purpose of this study was to evaluate the antibacterial activities of isolated lytic bacteriophage against ciprofloxacin-resistant strain of Salmonella infanits in vitro conditions.
Materials and Methods: The standard strain of Salmonella infantis  and its specific bacteriophage was isolated by soft agar method. Phage susceptibility to heat and pH was evaluated by the Double-Layer Agar method.  In vitro assay was carried out on human epithelial type 2 (HEp-2) cells to investigate the effect of bacteriophage on the cytotoxic and invasion of Salmonella infantis to human epithelial cells.
Results: Head and tail morphology of bacteriophages against Salmonella infantis were identified by transmission electron microscopy and assigned to the Myoviridae family. The results of the double-layer agar assay showed that the titer of bacteriophages was 1.8×107 PFU/ml. bacteriophage was stable at 4 ֯C and the best quantification of bacteriophage was determined at pH=8. The isolated bacteriophage was specific for Salmonella infantis and had no lytic activity against other pathogenic bacteria. In the evaluation of the binding and invasion of Salmonella infantis to the HEp-2 cell line, as expected, the lytic activity of specific bacteriophage was observed following inoculation.
Conclusion: Additional studies are needed for better understanding of the interaction between phage, microorganisms and human host before applying phage therapy on a large scale.

Keywords: Salmonella, Salmonellosis, Lytic bacteriophage, Ciprofloxacin
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✅ Additional studies are needed for better understanding of the interaction between phage, microorganisms and human host before applying phage therapy on a large scale.


Type of Study: Original Article | Subject: Medical Biology
Received: 2020/11/1 | Accepted: 2021/03/14 | Published: 2022/01/31
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