Background & Objective: Bone morphogenetic protein-9 (BMP-9) plays a pivotal role in regulating obesity, glucose homeostasis, and complications of metabolic syndrome (MetS), particularly in the context of estrogen deficiency. The aim of this study was to investigate the effects of eight weeks of high-intensity interval training on BMP-9 levels, insulin resistance (HOMA-IR), lipid profile, body composition, and components of metabolic syndrome in ovariectomized Wistar rats.
 Materials & Methods: Twenty-one Wistar rats (200–220 g) were randomly assigned into three equal groups (n=7): ovariectomized sedentary (OVX+Sal), ovariectomized with HIIT (OVX+HIIT), and sham-operated (Sham). The HIIT group underwent eight weeks of training (three sessions/week). Body weight, BMI, waist circumference, visceral fat, lipid profile, HOMA-IR, and BMP-9 levels were measured 24 hours post-training.
Results:  Statistical analysis was performed using one-way ANOVA followed by Tukey’s post hoc test, with significance set at P < 0.05. The HIIT intervention resulted in significant elevations in HDL and BMP-9 concentrations (P = 0.001) compared to the OVX+Sal group.
Conclusion:  HIIT attenuates components of MetS in ovariectomized rats by increasing BMP-9 levels and improving insulin sensitivity, thereby suggesting a potential role for exercise in managing postmenopausal metabolic health.