Articles In Press                   Back to the articles list | Back to browse issues page

Ethics code: IR.DUMS.REC.1401.046

XML Print

Ameliorative Role of Resveratrol in Silver Nanoparticle–Induced Oxidative Stress and Testicular Alterations in Male Mice
Mohammad amin Behmanesh1 , Sima Janati2 , Rezvan Reyhani3 , Seyedeh Mahsa Poormoosavi *4
1- 1. Department of Histology, School of Medicine, Dezful University of Medical Sciences, Dezful, Iran
2- 2. Department of Obstetrics and Gynecology, School of Medicine, Research and Clinical Center for Infertility, Dezful University of Medical Sciences, Dezful, Iran
3- 3. Student research committee, Dezful University of medical science, Dezfu, Iran
4- 4. Department of Histology, School of Medicine, Research and Clinical Center for Infertility Dezful University of Medical Sciences, Dezful, Iran , m.poormosavi@ymail.com
Abstract:   (117 Views)
Background: Silver nanoparticles (AgNPs) are among the most commonly used silver derivatives, which, due to their potential reproductive toxicity, can be considered a potential hazard for fertility.
Objective: The study aimed to evaluate the protective effect of resveratrol against AgNPs-induced testicular damage and oxidative stress in male mice model.
Materials and Methods: In this experimental study, 24 adult male mice were randomly divided into four groups (n=6/group): control, AgNPs (200 mg/kg), resveratrol (20 mg/kg), and a combination of AgNPs and resveratrol. Treatments were administered via daily gavage for 35 days. Finaly, serum malondialdehyde (MDA), total antioxidant capacity (TAC), superoxide dismutase (SOD), testosterone, testicular 8-OHdG levels, and histological changes included seminiferous tubule diameter, Leydig cell count, tissue structure, and spermatogenic status were analyzed between groups.
Results: AgNPs significantly reduced serum testosterone levels and increased oxidative stress, as indicated by elevated SOD and 8-OHdG levels (P< 0.001). Resveratrol treatment significantly restored testosterone levels and reduced oxidative DNA damage, with values approaching those of the control group (P< 0.01). No significant changes were observed in TAC and MDA levels among the groups. AgNPs caused marked reductions in seminiferous tubule diameter, epithelial thickness, and counts of primary spermatocytes, spermatozoa, Sertoli, and Leydig cells (P<0.001). These adverse effects were significantly ameliorated by Resveratrol, particularly in the Resveratrol-only group (P<0.01).
Conclusion: AgNPs induce oxidative stress-mediated testicular damage and hormonal disruption in male mice. While resveratrol exerts a protective role by mitigating some of these effects, its impact was limited and did not fully reverse AgNP-induced toxicity.
 
Keywords: Silver nanoparticles, Resveratrol, Reproductive Toxicity, Testicular Damage, Mice.Mice.
     
Type of Study: Original Research Article | Subject: Pharmacology
Received: 2025/09/8 | Accepted: 2026/06/22
References
1. Khan I, Saeed K, Khan I. Nanoparticles: Properties, applications and toxicities. Arab J Chem. 2019;12(7):908-31. [DOI:10.1016/j.arabjc.2017.05.011]
2. Malani PN. Harrison's principles of internal medicine. Jama. 2012;308(17):1813-4. [DOI:10.1001/jama.308.17.1813-b]
3. Tortella G, Rubilar O, Durán N, Diez M, Martínez M, Parada J, et al. Silver nanoparticles: Toxicity in model organisms as an overview of its hazard for human health and the environment. J Hazard Mater. 2020;390:121974. [DOI:10.1016/j.jhazmat.2019.121974] [PMID]
4. Das A, Roychoudhury S. Reactive oxygen species in the reproductive system: sources and physiological roles. In oxidative stress and toxicity in reproductive biology and medicine: A comprehensive update on male infertility 2022(1)(9-40). Cham: Springer International Publishing. [DOI:10.1007/978-3-030-89340-8_2] [PMID]
5. Takalani NB, Monageng EM, Mohlala K, Monsees TK, Henkel R, Opuwari CS. Role of oxidative stress in male infertility. Reprod Fertil. 2023 1;4(3). [DOI:10.1530/RAF-23-0024] [PMID] [PMCID]
6. Chakraborty S, Roychoudhury S. Pathological roles of reactive oxygen species in male reproduction. In oxidative stress and toxicity in reproductive biology and medicine: A comprehensive update on male infertility 2022 (1) (41-62). Cham: Springer International Publishing. [DOI:10.1007/978-3-030-89340-8_3] [PMID]
7. Hadrup N, Lam HR. Oral toxicity of silver ions, silver nanoparticles and colloidal silver-a review. Regul Toxicol Pharmacol. 2014;68(1):1-7. [DOI:10.1016/j.yrtph.2013.11.002] [PMID]
8. Meher A, Tandi A, Moharana S, Chakroborty S, Mohapatra SS, Mondal A,et al. Silver nanoparticle for biomedical applications: A review. Hybrid Adv. 2024;6:100184. [DOI:10.1016/j.hybadv.2024.100184]
9. Aziz MA, Diab AS. Antioxidant categories and mode. Antioxidants. 2019:3.
10. Park EJ, Bae E, Yi J, Kim Y, Choi K, Lee SH, et al. Repeated-dose toxicity and inflammatory responses in mice by oral administration of silver nanoparticles. Environment Toxicol Pharmacol. 2010;30(2):162-8. [DOI:10.1016/j.etap.2010.05.004] [PMID]
11. Hamza RZ, Al-Harbi MS, Al-Hazaa MA. Neurological alterations and testicular damages in aging induced by D-galactose and neuro and testicular protective effects of combinations of chitosan nanoparticles, resveratrol and quercetin in male mice. Coatings. 2021;11(4):435. [DOI:10.3390/coatings11040435]
12. Saleh RA, HCLD AA. Oxidative stress and male infertility: from research bench to clinical practice. J Androl. 2002;23(6):737-52. [DOI:10.1002/j.1939-4640.2002.tb02324.x]
13. Özatik FY, Özatik O, Yiğitaslan S, Ünel ÇÇ, Erol K. Protective role of resveratrol on testicular germ cells in mice with testicular toxicity. Turk J Urol. 2017;43(4):444. [DOI:10.5152/tud.2017.34101] [PMID] [PMCID]
14. Assar DH, Mokhbatly AA, MFA EL, Ghazy EW, Gaber AA, Elbialy ZI, et al. Silver nanoparticles induced testicular damage targeting NQO1 and APE1 dysregulation, apoptosis via Bax/Bcl-2 pathway, fibrosis via TGF-β/α-SMA upregulation in rats. Environment Sci Pollut Res Int. 2023;30(10):26308-26. [DOI:10.1007/s11356-022-23876-y] [PMID] [PMCID]
15. Fathi N, Hoseinipanah SM, Alizadeh Z, Assari MJ, Moghimbeigi A, Mortazavi M, et al. The effect of silver nanoparticles on the reproductive system of adult male rats: A morphological, histological and DNA integrity study. Adv Clin Experimen Med. 2019;28(3):299-305. [DOI:10.17219/acem/81607] [PMID]
16. Ansar S, Alshehri SM, Abudawood M, Hamed SS, Ahamad T. Antioxidant and hepatoprotective role of selenium against silver nanoparticles. Int J Nanomed. 2017;12:7789-97. [DOI:10.2147/IJN.S136748] [PMID] [PMCID]
17. Lopes IMD, de Oliveira IM, Bargi-Souza P, Cavallin MD, Kolc CSM, Khalil NM, et al. Effects of silver nanoparticle exposure to the testicular antioxidant system during the prepubertal rat stage. Chem Res Toxicol. 2019;32(6):986-94. [DOI:10.1021/acs.chemrestox.8b00281] [PMID]
18. Fukui M, Choi HJ, Zhu BT. Mechanism for the protective effect of resveratrol against oxidative stress-induced neuronal death. Free Radic Biol Med. 2010;49(5):800-13. [DOI:10.1016/j.freeradbiomed.2010.06.002] [PMID] [PMCID]
19. Wang XL, Li T, Li JH, Miao SY, Xiao XZ. The effects of resveratrol on inflammation and oxidative stress in a rat model of chronic obstructive pulmonary disease. Molecules. 2017;22(9):1529. [DOI:10.3390/molecules22091529] [PMID] [PMCID]
20. Mojica-Villegas MA, Izquierdo-Vega JA, Chamorro-Cevallos G, Sánchez-Gutiérrez M. Protective effect of resveratrol on biomarkers of oxidative stress induced by iron/ascorbate in mouse spermatozoa. Nutrients. 2014;6(2):489-503. [DOI:10.3390/nu6020489] [PMID] [PMCID]
Send email to the article author

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

© 2026 CC BY-NC 4.0 | Journal of Advances in Medical and Biomedical Research

Designed & Developed by : Yektaweb