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Volume 34, Issue 3 (May & June 2026)
J Adv Med Biomed Res 2026, 34(3): 261-268 |
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Hussein A A M, Kadhim E F. Histological evaluation of the effect of local application of angiopoietin 1 on tooth development in rats on days 16. J Adv Med Biomed Res 2026; 34 (3) :261-268
URL: http://journal.zums.ac.ir/article-1-7931-en.html
URL: http://journal.zums.ac.ir/article-1-7931-en.html
1- College Of Dentistry, University Of Al-Ameed, kerbala, Iraq , alhusain@alameed.edu.iq
2- College Of Dentistry, University Of Baghdad, Baghdad, Iraq
2- College Of Dentistry, University Of Baghdad, Baghdad, Iraq
Abstract: (361 Views)
Background & Objective: Angiopoietin-1 (Ang-1) is a key regulator of angiogenesis and vascular maturation and may play an important role in tooth development. This histological study aimed to evaluate the effect of the local application of angiopoietin-1 on tooth development in the upper molar region of neonatal rats at postnatal day 16.
Materials & Methods: Twelve neonatal rats weighing 3.5–4.0 g were maintained under controlled environmental conditions with free access to food and water. The animals were randomly allocated to either an experimental group (n = 6) or a control group (n = 6). The experimental group received a local injection of 10 μL of angiopoietin-1 into the upper right molar region, whereas the control group received an equal volume of sterile saline. All animals were sacrificed on postnatal day 16, and histological analyses were performed to evaluate tooth development.
Results: Local administration of angiopoietin-1 significantly accelerated tooth development in all animals of the experimental group. Histological examination demonstrated enhanced deposition of dental hard tissues, characterized by increased dentin and enamel thickness, together with significantly higher numbers of odontoblasts, fibroblasts, and blood vessels compared with the control group. All evaluated histological parameters differed significantly between the two groups (P < 0.05).
Conclusion: Local application of angiopoietin-1 promoted and accelerated tooth development in neonatal rats, suggesting that angiopoietin-1 may enhance odontogenesis by stimulating angiogenesis and promoting the formation and maturation of dental tissues.
Materials & Methods: Twelve neonatal rats weighing 3.5–4.0 g were maintained under controlled environmental conditions with free access to food and water. The animals were randomly allocated to either an experimental group (n = 6) or a control group (n = 6). The experimental group received a local injection of 10 μL of angiopoietin-1 into the upper right molar region, whereas the control group received an equal volume of sterile saline. All animals were sacrificed on postnatal day 16, and histological analyses were performed to evaluate tooth development.
Results: Local administration of angiopoietin-1 significantly accelerated tooth development in all animals of the experimental group. Histological examination demonstrated enhanced deposition of dental hard tissues, characterized by increased dentin and enamel thickness, together with significantly higher numbers of odontoblasts, fibroblasts, and blood vessels compared with the control group. All evaluated histological parameters differed significantly between the two groups (P < 0.05).
Conclusion: Local application of angiopoietin-1 promoted and accelerated tooth development in neonatal rats, suggesting that angiopoietin-1 may enhance odontogenesis by stimulating angiogenesis and promoting the formation and maturation of dental tissues.
Type of Study: Original Research Article |
Subject:
Life Science
Received: 2026/02/7 | Accepted: 2026/06/2 | Published: 2026/06/29
Received: 2026/02/7 | Accepted: 2026/06/2 | Published: 2026/06/29
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