شنبه 17 خرداد 1404
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دوره 19، شماره 74 - ( 1-1390 )
جلد 19 شماره 74 صفحات 36-25 |
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Darabian S, Rezaie S, Kordbachche P, Safari M R. The Effect of Variable CO2 Concentrations on HSP70 Gene in Trichophyton Rubrum and Microsporeum Canis. J Adv Med Biomed Res 2011; 19 (74) :25-36
URL: http://journal.zums.ac.ir/article-1-1413-fa.html
URL: http://journal.zums.ac.ir/article-1-1413-fa.html
دارابیان سیما، رضایی ساسان، کردبچه پریوش، صفری محمد رضا. بررسی تاثیر غلظت‌های مختلف CO2 در بیان ژن HSP70 در قارچ‌های درماتوفیت ترایکوفایتون روبروم و میکروسپوروم کانیس. Journal of Advances in Medical and Biomedical Research. 1390; 19 (74) :25-36
سیما دارابیان*1 
، ساسان رضایی2 ، پریوش کردبچه3 ، محمد رضا صفری4
، ساسان رضایی2 ، پریوش کردبچه3 ، محمد رضا صفری4
1- کارشناس ارشد قارچ شناسی پزشکی، عضو هیئت علمی دانشگاه علوم پزشکی زنجان ، dsima2004@yahoo.com
2- دکترای تخصصی قارچ شناسی پزشکی، دانشیار دانشگاه علوم پزشکی تهران
3- دکترای تخصصی قارچ شناسی پزشکی، استاد دانشگاه علوم پزشکی تهران
4- کارشناش علوم آزمایشگاهی، دانشگاه علوم پزشکی تهران
2- دکترای تخصصی قارچ شناسی پزشکی، دانشیار دانشگاه علوم پزشکی تهران
3- دکترای تخصصی قارچ شناسی پزشکی، استاد دانشگاه علوم پزشکی تهران
4- کارشناش علوم آزمایشگاهی، دانشگاه علوم پزشکی تهران
چکیده: (169214 مشاهده)
Background and Objectivs: Trichophyton rubrum is considered as the most common causes of dermatophytosis in human skin and nail tissues. Microsporeum canis is a zoophile dermatophyte which can be transmited to human. HSP70 is a 70 KD heat shock protein in fungi. In this study, the effects of variable CO2 concentrations were examined on HSP70 expression in T. rubrum and M. canis.
Materials and Methods: Strains used in this study were obtained from skin scales and nails of the patients who were suffering from onychomycosis. Samples were cultured on Sabouraud dextrose broth (SDB) and incubated at 25°C for 2, 4 and 7 days under 3%, 5%, and 10% of CO2 concentrations. Control cultures maintained for 7 days without CO2 concentrations. Then, RNA was isolated from the harvested mycelia mass, and HSP70 gene expression was studied in T. rubrum and M. canis by RT-PCR. The obtained results were compared to the Beta actin as a house keeping gene.
Results: The results of this study revealed the maximum variations under 3%,5%, and 10% of CO2 concentrations in maximum 7 days incubation period, and the expression of HSP70 gene showed different variations under different CO2 concentrations.
Conclusion: Our results showed a negative effect of CO2 concentrations in the expression of HSP70 in T. Rubrum and a positive effect in M. canis comparing to the controls.
نوع مطالعه: مقاله پژوهشی |
دریافت: 1389/12/17 | پذیرش: 1393/4/2 | انتشار: 1393/4/2
دریافت: 1389/12/17 | پذیرش: 1393/4/2 | انتشار: 1393/4/2
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