Volume 19, Issue 74 (3-2011)                   J Adv Med Biomed Res 2011, 19(74): 25-36 | Back to browse issues page

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Darabian S, Rezaie S, Kordbachche P, Safari M R. The Effect of Variable CO2 Concentrations on HSP70 Gene in Trichophyton Rubrum and Microsporeum Canis. J Adv Med Biomed Res 2011; 19 (74) :25-36
URL: http://journal.zums.ac.ir/article-1-1413-en.html
The Effect of Variable CO2 Concentrations on HSP70 Gene in Trichophyton Rubrum and Microsporeum Canis
Sima Darabian *1 , Sasan Rezaie2 , Parinoush Kordbachche2 , Mohammad Reza Safari2
1- School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran , dsima2004@yahoo.com
2- Division of Molecular Biology, Dept. of Medical Mycology & Parasitology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
Abstract:   (169270 Views)

Background and Objectivs: Trichophyton rubrum is considered as the most common causes of dermatophytosis in human skin and nail tissues. Microsporeum canis is a zoophile dermatophyte which can be transmited to human. HSP70 is a 70 KD heat shock protein in fungi. In this study, the effects of variable CO2 concentrations were examined on HSP70 expression in T. rubrum and M. canis.
Materials and Methods: Strains used in this study were obtained from skin scales and nails of the patients who were suffering from onychomycosis. Samples were cultured on Sabouraud dextrose broth (SDB) and incubated at 25°C for 2, 4 and 7 days under 3%, 5%, and 10% of CO2 concentrations. Control cultures maintained for 7 days without CO2 concentrations. Then, RNA was isolated from the harvested mycelia mass, and HSP70 gene expression was studied in T. rubrum and M. canis by RT-PCR. The obtained results were compared to the Beta actin as a house keeping gene.
Results: The results of this study revealed the maximum variations under 3%,5%, and 10% of CO2 concentrations in maximum 7 days incubation period, and the expression of HSP70 gene showed different  variations  under different CO2 concentrations.
Conclusion: Our results showed a negative effect of CO2 concentrations in the expression of HSP70 in T. Rubrum and a positive effect in M. canis comparing to the controls.

Keywords: Dermatophyte, Trichophyton rubrum, Microsporeum canis, HSP70 Gene
Full-Text [PDF 790 kb]   (162873 Downloads)    
Type of Study: Original Research Article |
Received: 2011/03/8 | Accepted: 2014/06/23 | Published: 2014/06/23
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