دوره 19، شماره 74 - ( 1-1390 )                   جلد 19 شماره 74 صفحات 53-44 | برگشت به فهرست نسخه ها

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Haniloo A, Najafi F, Fazaeli A, Nourian A A. Comparison and Evaluation of Echinococcus granulosus Protoscoleces Excretory/Secretory Proteins in PBS Complemented with Glucose, DMEM and RPMI Culture Media. J Adv Med Biomed Res 2011; 19 (74) :44-53
URL: http://journal.zums.ac.ir/article-1-1416-fa.html
هانیلو علی، نجفی فائزه، فضائلی اصغر، نوریان عباس علی. مقایسه و ارزیابی تولید پروتئین‎های دفعی- ترشحی پروتواسکولکس اکینوکوکوس گرانولوزوس در محیط‎های کشتRPMI ، DMEM و PBS غنی شده با گلوکز. Journal of Advances in Medical and Biomedical Research. 1390; 19 (74) :44-53

URL: http://journal.zums.ac.ir/article-1-1416-fa.html


1- دکترای تخصصی انگل‌شناسی، دانشیار دانشگاه علوم پزشکی زنجان
2- کارشناس ارشد انگل‌شناسی، دانشکده پزشکی دانشگاه علوم پزشکی زنجان ، faezeh_najafi@yahoo.com
چکیده:   (167355 مشاهده)

Background and Objective: Preparation of proper antigens is an important issue in serology of hydatidosis. Investigators have been able to obtain excretory/secretory antigens (E/S Ags) by short-term culture of protoscoleces in a couple of culture media. However, no data are available about production rate of such antigens in different culture media. The present study was carried out to evaluate the production of E/S Ags (proteins) in PBS complemented with glucose, DMEM and RPMI culture media.
Material and Methods: To obtain E/S proteins, protoscoleces of echinococcus were cultured in PBS complemented with 10% glucose, RPMI and DMEM for 72 hours. Proteins secreted in culture media were concentrated and assayed. To characterize different components, proteins were electrophoresed on SDS-PAGE. Data were analyzed using One-way ANOVA and Tukey HSD tests.
Results: The mean concentration of E/S proteins in PBS medium in 24 hours of culture was significantly higher than DMEM and RPMI (P<0.05). However, such a difference was not observed between E/S proteins in DMEM and RPMI media. E/S proteins obtained from PBS medium were separated into 12 major bands and the two other media into 14 major bands within a range of molecular masses of 16 to 67 kDa.
Conclusion: PBS complemented with glucose is more appropriate than the two other media for E/S proteins production. The best time to obtain E/S proteins is the first 48 hours of culture.

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نوع مطالعه: مقاله پژوهشی |
دریافت: 1389/12/17 | پذیرش: 1393/4/2 | انتشار: 1393/4/2

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