Volume 19, Issue 74 (3-2011)                   J Adv Med Biomed Res 2011, 19(74): 44-53 | Back to browse issues page

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Haniloo A, Najafi F, Fazaeli A, Nourian A A. Comparison and Evaluation of Echinococcus granulosus Protoscoleces Excretory/Secretory Proteins in PBS Complemented with Glucose, DMEM and RPMI Culture Media. J Adv Med Biomed Res 2011; 19 (74) :44-53
URL: http://journal.zums.ac.ir/article-1-1416-en.html
Comparison and Evaluation of Echinococcus granulosus Protoscoleces Excretory/Secretory Proteins in PBS Complemented with Glucose, DMEM and RPMI Culture Media
Ali Haniloo1 , Faezeh Najafi *2 , Asghar Fazaeli1 , Ali Abbas Nourian1
1- Dept. of Parasitology and Entomology, Faculty of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran.
2- Dept. of Parasitology and Entomology, Faculty of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran. , faezeh_najafi@yahoo.com
Abstract:   (167357 Views)

Background and Objective: Preparation of proper antigens is an important issue in serology of hydatidosis. Investigators have been able to obtain excretory/secretory antigens (E/S Ags) by short-term culture of protoscoleces in a couple of culture media. However, no data are available about production rate of such antigens in different culture media. The present study was carried out to evaluate the production of E/S Ags (proteins) in PBS complemented with glucose, DMEM and RPMI culture media.
Material and Methods: To obtain E/S proteins, protoscoleces of echinococcus were cultured in PBS complemented with 10% glucose, RPMI and DMEM for 72 hours. Proteins secreted in culture media were concentrated and assayed. To characterize different components, proteins were electrophoresed on SDS-PAGE. Data were analyzed using One-way ANOVA and Tukey HSD tests.
Results: The mean concentration of E/S proteins in PBS medium in 24 hours of culture was significantly higher than DMEM and RPMI (P<0.05). However, such a difference was not observed between E/S proteins in DMEM and RPMI media. E/S proteins obtained from PBS medium were separated into 12 major bands and the two other media into 14 major bands within a range of molecular masses of 16 to 67 kDa.
Conclusion: PBS complemented with glucose is more appropriate than the two other media for E/S proteins production. The best time to obtain E/S proteins is the first 48 hours of culture.

Keywords: Echinococcus granulosus, Protoscoleces, Excretory/Secretory antigens
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Type of Study: Original Research Article |
Received: 2011/03/8 | Accepted: 2014/06/23 | Published: 2014/06/23
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