پنجشنبه 15 خرداد 1404
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دوره ۲۶، شماره ۱۱۶ - ( ۵-۱۳۹۷ )
جلد ۲۶ شماره ۱۱۶ صفحات ۱۹-۹ |
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Doustvandi M A, Mohammadnejad F, Mashayekhi M R. Investigation of Caspase 9 Gene Expression and Apoptosis Induction after Photodynamic Therapy with Zinc Phthalocyanine in SW872 Skin Cancer Cell Line. J Adv Med Biomed Res 2018; 26 (116) :9-19
URL: http://journal.zums.ac.ir/article-1-5084-fa.html
URL: http://journal.zums.ac.ir/article-1-5084-fa.html
دوستوندی محمدامین، محمدنژاد فاطمه، مشایخی محمد‌رضا. بررسی بیان ژن کاسپاز ۹ و القای آپوپتوز پس از فوتودینامیک تراپی با روی فتالوسیانین در رده‌ی سلولیSW۸۷۲ سرطان پوست. Journal of Advances in Medical and Biomedical Research. ۱۳۹۷; ۲۶ (۱۱۶) :۹-۱۹
۱- دانشگاه آزاد اسلامی واحد تبریز، تبریز ، m.mashayekhi@iaut.ac.ir
۲- دانشگاه آزاد اسلامی واحد تبریز، تبریز
۲- دانشگاه آزاد اسلامی واحد تبریز، تبریز
چکیده: (۱۶۵۳۲۶ مشاهده)
Background and Objective: The treatment of cancer comes as a great challenge worldwide. Thus the development of effective therapies with minimal side effects is important. Photodynamic therapy is a non-invasive and new therapeutic approach for the treatment of cancer. Therefore, in this study, we evaluated the photodynamic effects of a light-sensitive compound, Zinc-phthalocyanine (ZnPc), on cell growth and the expression of caspase-9 in SW872 cell line.
Materials and Methods: For cytotoxic effects of photodynamic therapy with ZnPc, MTT assay was conducted. Quantifying the level of caspase-9 gene expression was carried out with qRT-PCR. The statistical analysis was done by GraphPad Prism 6 software.
Results: MTT assay results showed that photodynamic therapy with ZnPc significantly reduced cancer cell growth. The IC50 for cell line SW872 was 0/034 μg/ml in a 24 J/cm2 light source. qRT-PCR results showed that photodynamic therapy with ZnPc increased the gene expression of caspase-9 in the treated group.
Conclusion: Photodynamic therapy with ZnPc resulted in decreased cell growth and induced apoptosis in a concentration-dependent manner in SW872 cell lines. Based on the results, photodynamic therapy with ZnPc can be a powerful therapeutic approach for the treatment of skin cancer with minimal side effects.
نوع مطالعه: کارآزمایی بالینی |
دریافت: 1396/12/9 | پذیرش: 1396/12/9 | انتشار: 1396/12/9
دریافت: 1396/12/9 | پذیرش: 1396/12/9 | انتشار: 1396/12/9
فهرست منابع
1. References
2. Lucky SS, Soo KC, Zhang Y. Nanoparticles in photodynamic therapy. Chemical Rev. 2015; 115: 1990-2042. [DOI:10.1021/cr5004198] [PMID]
3. Hanahan D, Weinberg RA. The hallmarks of cancer. Cell. 2000; 100: 57-70. [DOI:10.1016/S0092-8674(00)81683-9]
4. Omar S, Alieldin N, Khatib O. Cancer magnitude, challenges and control in the Eastern Mediterranean region. 2007. [DOI:10.26719/2007.13.6.1486] [PMID]
5. Diepgen TL, Mahler V. The epidemiology of skin cancer. Br J Dermatol. 2002; 146: 1-6. [DOI:10.1046/j.1365-2133.146.s61.2.x] [PMID]
6. Marjani A, Kabir MJ. Male skin cancer incidence in Golestan province, Iran. J Pakistan Med Assoc. 2009; 59: 287. [DOI:10.4103/0019-509X.55564] [PMID]
7. Rigel DS, Carucci JA. Malignant melanoma: prevention, early detection, and treatment in the 21st century. CA: Cancer J Clin. 2000; 50: 215-36. [DOI:10.3322/canjclin.50.4.215] [PMID]
8. Ghoncheh M, Koohi F, Salehiniya H. Epidemiology and trend of skin cancer incidence in southern Iran. J Dermatol Cosmet. 2015; 6:
9. Keyghobadi N, Rafiemanesh H, Mohammadian-Hafshejani A, Enayatrad M, Salehiniya H. Epidemiology and trend of cancers in the province of Kerman: southeast of Iran. Asian Pac J Cancer Prev. 2015; 16: 1409-13. [DOI:10.7314/APJCP.2015.16.4.1409] [PMID]
10. Mackie R, Quinn A. Non‐melanoma skin cancer and other epidermal skin tumours. Rook's Textbook of Dermatology, Seventh Edition. 2004: 1801-50. [DOI:10.1002/9780470750520.ch36]
11. Mirzaei M, Razi S, Ghoncheh M, Mohammadian Hafshejani A, Salehiniya H. Skin cancer incidence rate and trend in 2004-2008 in Tehran province. J Dermatol Cosmet. 2015; 5: 193-9.
12. Yano S, Hirohara S, Obata M, et al. Current states and future views in photodynamic therapy. Photochem Rev. 2011; 12: 46-67. [DOI:10.1016/j.jphotochemrev.2011.06.001]
13. Spyratou E, Makropoulou M, Mourelatou E, Demetzos C. Biophotonic techniques for manipulation and characterization of drug delivery nanosystems in cancer therapy. Cancer letters. 2012; 327: 111-22. [DOI:10.1016/j.canlet.2011.12.039] [PMID]
14. Prasad PN. Introduction to biophotonics: John Wiley & Sons; 2004.
15. Sharifi M, Moosavi M, Naji T. Effect of nitrogen-nano doped of titanium dioxide in human A-375 melanoma cancer cell line. 2017.
16. Mroz P, Yaroslavsky A, Kharkwal GB, Hamblin MR. Cell death pathways in photodynamic therapy of cancer. Cancers. 2011; 3: 2516-39. [DOI:10.3390/cancers3022516] [PMID] [PMCID]
17. Navaeipour F, Afsharan H, Tajalli H, et al. Effects of continuous wave and fractionated diode laser on human fibroblast cancer and
18. dermal normal cells by zinc phthalocyanine in photodynamic therapy: A comparative study.J Photochem Photobiol. 2016; 161: 456-62. [DOI:10.1016/j.jphotobiol.2016.06.017] [PMID]
19. Deda DK, Araki K. Nanotechnology, Light and chemical action: an effective combination to kill cancer cells. J Brazil Chem Soc. 2015; 26: 2448-70. [DOI:10.5935/0103-5053.20150316]
20. Cortese DA, Kinsey JH. Hematoporphyrin derivative phototherapy in the treatment of bronchogenic carcinoma. Chest. 1984; 86: [DOI:10.1378/chest.86.1.8] [PMID]
21. Hayata Y, Kato H, Konaka C, Ono J, Takizawa N. Hematoporphyrin derivative and laser photoradiation in the treatment of lung cancer. Chest. 1982; 81: 269-77. [DOI:10.1378/chest.81.3.269] [PMID]
22. Hill JS, Kaye AH, Sawyer WH, Morstyn G, Megison PD, Stylli SS. Selective uptake of hematoporphyrin derivative into human cerebral glioma. Neurosurgery. 1990; 26: 248-54. [DOI:10.1227/00006123-199002000-00011] [PMID]
23. Laws Jr ER, Cortese DA, Kinsey JH, Lagan RT, Anderson RL. Photoradiation therapy in the treatment of malignant brain tumors: a Phase I (feasibility) study. Neurosurgery. 1981; 9: 672-8. [DOI:10.1227/00006123-198112000-00010] [PMID]
24. Schweitzer VG. Photodynamic therapy for treatment of head and neck cancer. Otolaryngology Surg. 1990; 102: 225-32. [DOI:10.1177/019459989010200304] [PMID]
25. Wenig BL, Kurtzman DM, Grossweiner LI, et al. Photodynamic therapy in the treatment of squamous cell carcinoma of the head and neck. 1990; 116: 1267-70. [DOI:10.1001/archotol.1990.01870110039003] [PMID]
26. Barnes LD, Giuliano EA, Ota J, Cohn LA, Moore CP. The effect of photodynamic therapy on squamous cell carcinoma in a murine model: evaluation of time between intralesional injection to laser irradiation. Veterinary J. 2009; 180: 60-5. [DOI:10.1016/j.tvjl.2007.11.023] [PMID]
27. Debele TA, Peng S, Tsai H-C. Drug carrier for photodynamic cancer therapy. Int J Molec Sci. 2015; 16: 22094-136. [DOI:10.3390/ijms160922094] [PMID] [PMCID]
28. Feuser PE, Gaspar PC, Jacques AV, et al. Synthesis of ZnPc loaded poly (methyl methacrylate) nanoparticles via miniemulsion polymerization for photodynamic therapy in leukemic cells. Material Sci Engin: C. 2016; 60: 458-66. [DOI:10.1016/j.msec.2015.11.063] [PMID]
29. Soriano J, Villanueva A, Stockert JC, Cañete M. Regulated necrosis in HeLa cells induced by ZnPc photodynamic treatment: A new nuclear morphology. Int J Molec Sci. 2014; 15: 22772-85. [DOI:10.3390/ijms151222772] [PMID] [PMCID]
30. Vittar NBR, Awruch J, Azizuddin K, Rivarola V. Caspase-independent apoptosis, in human MCF-7c3 breast cancer cells, following photodynamic therapy, with a novel water-soluble phthalocyanine. Int J Biochem Cell Biol. 2010; 42: 1123-31. [DOI:10.1016/j.biocel.2010.03.019] [PMID]
31. Shao J, Dai Y, Zhao W, et al. Intracellular distribution and mechanisms of actions of photosensitizer Zinc (II)-phthalocyanine solubilized in Cremophor EL against human hepatocellular carcinoma HepG2 cells. Cancer letters. 2013; 330: 49-56. [DOI:10.1016/j.canlet.2012.11.017] [PMID]
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