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Volume 26, Issue 116 (7-2018)
J Adv Med Biomed Res 2018, 26(116): 9-19 |
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Doustvandi M A, Mohammadnejad F, Mashayekhi M R. Investigation of Caspase 9 Gene Expression and Apoptosis Induction after Photodynamic Therapy with Zinc Phthalocyanine in SW872 Skin Cancer Cell Line. J Adv Med Biomed Res 2018; 26 (116) :9-19
URL: http://journal.zums.ac.ir/article-1-5084-en.html
URL: http://journal.zums.ac.ir/article-1-5084-en.html
1- Dept. of Genetics, Islamic Azad University, Tabriz Branch, Tabriz, Iran , m.mashayekhi@iaut.ac.ir
2- Dept. of Genetics, Islamic Azad University, Tabriz Branch, Tabriz, Iran
2- Dept. of Genetics, Islamic Azad University, Tabriz Branch, Tabriz, Iran
Abstract: (165373 Views)
Background and Objective: The treatment of cancer comes as a great challenge worldwide. Thus the development of effective therapies with minimal side effects is important. Photodynamic therapy is a non-invasive and new therapeutic approach for the treatment of cancer. Therefore, in this study, we evaluated the photodynamic effects of a light-sensitive compound, Zinc-phthalocyanine (ZnPc), on cell growth and the expression of caspase-9 in SW872 cell line.
Materials and Methods: For cytotoxic effects of photodynamic therapy with ZnPc, MTT assay was conducted. Quantifying the level of caspase-9 gene expression was carried out with qRT-PCR. The statistical analysis was done by GraphPad Prism 6 software.
Results: MTT assay results showed that photodynamic therapy with ZnPc significantly reduced cancer cell growth. The IC50 for cell line SW872 was 0/034 μg/ml in a 24 J/cm2 light source. qRT-PCR results showed that photodynamic therapy with ZnPc increased the gene expression of caspase-9 in the treated group.
Conclusion: Photodynamic therapy with ZnPc resulted in decreased cell growth and induced apoptosis in a concentration-dependent manner in SW872 cell lines. Based on the results, photodynamic therapy with ZnPc can be a powerful therapeutic approach for the treatment of skin cancer with minimal side effects.
Type of Study: Clinical Trials |
Received: 2018/02/28 | Accepted: 2018/02/28 | Published: 2018/02/28
Received: 2018/02/28 | Accepted: 2018/02/28 | Published: 2018/02/28
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