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Volume 28, Issue 127 (March & April 2020)
J Adv Med Biomed Res 2020, 28(127): 105-110 |
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Charkhi P, Haghshenas M R, Mirzaei B, Davoodi L, Norouzi Bazgir Z, Goli H R. Comparison of the Effect of Phenylalanine Arginine Beta Naphthylamide (PAβN) and Curcumin on Minimum Inhibitory Concentration of Aminoglycosides on Pseudomonas aeruginosa Clinical Isolates. J Adv Med Biomed Res 2020; 28 (127) :105-110
URL: http://journal.zums.ac.ir/article-1-5935-en.html
URL: http://journal.zums.ac.ir/article-1-5935-en.html
Parisa Charkhi1 
, Mohammad Reza Haghshenas2 , Bahman Mirzaei3 , Lotfollah Davoodi4 , Zahra Norouzi Bazgir1 , Hamid Reza Goli * 5
, Mohammad Reza Haghshenas2 , Bahman Mirzaei3 , Lotfollah Davoodi4 , Zahra Norouzi Bazgir1 , Hamid Reza Goli * 5
1- Molecular and Cell Biology Research Centre, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran
2- Dept. of Medical Microbiology and Virology, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran
3- Dept. of Medical Microbiology and Virology, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran
4- Antimicrobial Resistance Research Center, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran
5- Molecular and Cell Biology Research Centre, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran ,goli59@gmail.com
2- Dept. of Medical Microbiology and Virology, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran
3- Dept. of Medical Microbiology and Virology, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran
4- Antimicrobial Resistance Research Center, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran
5- Molecular and Cell Biology Research Centre, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran ,
Abstract: (146678 Views)
Background and Objective: Efflux pump inhibitors (EPIs) can block efflux pumps and are helpful in potentiating the activity of aminoglycosides against Pseudomonas aeruginosa. The present study compared the effects of phenylalanine-arginine beta naphthylamide (PAβN) and curcumin on aminoglycoside minimum inhibitory concentration (MIC) on Pseudomonas aeruginosa clinical isolates.
Materials and Methods: For this descriptive-analytical study, 100 clinical isolates of Pseudomonas aeruginosa were collected and identified by differential diagnostic tests. The MICs of amikacin, gentamicin, and tobramycin were evaluated before and after adding EPIs using a micro-broth dilution test.
Results: The bacteria were isolated from different types of samples, including urine (26 isolates), sputum (37 isolates), ulcers (20 isolates), catheters (eight isolates), blood (five isolates), feces (two isolates), and eyes (two isolates). Overall, 60% of the isolates were obtained from males (mean age = 47.85), and 40% from females (mean age = 44.76).
In the MIC test, 11 (25.5%), 15 (34.8%), and 18 (41.8%) isolates were resistant to amikacin, gentamicin, and tobramycin, respectively. Significant reductions in the MICs of amikacin, gentamicin, and tobramycin were observed after adding curcumin in 54-100% of aminoglycoside-resistant isolates, while fewer changes in the MICs of aminoglycosides were seen against these clinical isolates after adding PAβN (36-55%).
Conclusion: Curcumin and PAβN can potentiate the effect of aminoglycosides on clinical isolates of Pseudomonas aeruginosa and change their susceptibility pattern due to efflux pump inhibition. However, our outcomes detected that curcumin was more effective than the PAβN against the aminoglycoside-resistant isolates of P. aeruginosa.
Keywords: Curcumin, Efflux pump, Inhibitor, Phenylalanine arginine beta naphthylamide, Pseudomonas aeruginosa
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✅ Curcumin and PAβN can potentiate the effect of aminoglycosides on clinical isolates of Pseudomonas aeruginosa and change their susceptibility pattern due to efflux pump inhibition. However, our outcomes detected that curcumin was more effective than the PAβN against the aminoglycoside-resistant isolates of P. aeruginosa.
Type of Study: Original Article |
Subject:
Medical Biology
Received: 2019/10/2 | Accepted: 2020/01/25 | Published: 2020/03/1
Received: 2019/10/2 | Accepted: 2020/01/25 | Published: 2020/03/1
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