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Nikbakht Rad M, Jafari Anarkooli I, Abdanipour A. Neuroprotective Effects of 1,8-Cineole on Apoptosis Inhibition and Bcl-2/Caspase-3 mRNA Expression in the Hippocampus of Epileptic Pilocarpine Model Rats. J Adv Med Biomed Res 2022; 30 (138) :30-38
URL: http://journal.zums.ac.ir/article-1-6294-en.html
Neuroprotective Effects of 1,8-Cineole on Apoptosis Inhibition and Bcl-2/Caspase-3 mRNA Expression in the Hippocampus of Epileptic Pilocarpine Model Rats
Mahsa Nikbakht Rad1 , Iraj Jafari Anarkooli * 2, Alireza Abdanipour1
1- Dept. of Anatomy, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran
2- Dept. of Anatomy, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran , anarkool@zums.ac.ir
Abstract:   (111491 Views)

Background and Objective: The neuroprotective potential of 1,8-Cineole (CIN) has recently been documented in vitro. Here we studied potential beneficial therapeutic effects of CIN, using the temporal lobe epilepsy (TLE) pilocarpine rat model through up-regulation of Bcl-2, as an anti-apoptotic gene.
Materials and Methods: A total of 32 (n=8 per group) male Wistar rats were divided into 4 groups as follows:  i) normal rats (received CIN (50 mg/kg)). ii) Non-treated epileptic rats. iii) Vehicle epileptic rats treated with 10% Polysorbate 20 (Tween 20). iv) TLE-treated rats with CIN once daily (50 mg/kg), three days after the first seizure and up to 28 days, four days a week (treatment group).
For the analysis, based on the Racine scale, the score of 4 and 5 was chosen. Rats were sacrificed and primed 28 days after the first seizure for both histopathological and quantitative real time PCR (qRT-PCR) analysis.
Results: The findings showed that CIN prevents cell death caused by Pilocarpine, via regulatory effect on apoptotic and anti-apoptotic gene expression. QRT-PCR results showed a significant increment in the Bcl-2 expression, and a decrease in Caspas-3 gene in the epileptic group treated with CIN. Also, amount of total antioxidant capacity was higher in CIN treated group. Histological study of the brain regions revealed a significant decrease in the apoptotic and necrotic hippocampal cells in the treatment groups.
Conclusion: Collectively, the present study showed CIN significantly induced neuroprotection effects for brain damage. It seems CIN can be a promising method for improving the effectiveness of therapy.

Keywords: 1, 8-cineole, Epilepsy, Pilocarpine, Bcl-2, Caspase-3
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✅ Collectively, the present study showed CIN significantly induced neuroprotection effects for brain damage. It seems CIN can be a promising method for improving the effectiveness of therapy.


Type of Study: Original Article | Subject: Medical Biology
Received: 2020/11/14 | Accepted: 2021/01/25 | Published: 2021/10/17
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