Volume 31, Issue 145 (March & April 2023)                   J Adv Med Biomed Res 2023, 31(145): 191-196 | Back to browse issues page

XML Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Wibisono D S, Sucahyo J F, Fulyani F, Ariani M D, Nugroho E A, Daniswara N. Effect of Temulawak (Curcuma xanthorrhiza) Extract on Sperm Counts, Morphology, and Motility of Nicotine-Induced Mice. J Adv Med Biomed Res 2023; 31 (145) :191-196
URL: http://journal.zums.ac.ir/article-1-6838-en.html
Effect of Temulawak (Curcuma xanthorrhiza) Extract on Sperm Counts, Morphology, and Motility of Nicotine-Induced Mice
Dimas Sindhu Wibisono *1 , Josephine Fiona Sucahyo2 , Faizah Fulyani3 , Mahayu Dewi Ariani3 , Eriawan Agung Nugroho4 , Nanda Daniswara4
1- Dept. of Urology, Kariadi Hospital, Faculty of Medicine, Diponegoro University, Semarang, Indonesia , uroandrology21@gmail.com
2- Faculty of Medicine, Diponegoro University, Semarang, Indonesia
3- Dept. of Medical Biology and Biochemistry, Faculty of Medicine, Diponegoro University, Semarang, Indonesia
4- Dept. of Urology, Kariadi Hospital, Faculty of Medicine, Diponegoro University, Semarang, Indonesia
Abstract:   (11576 Views)

Background and Objective: Infertility is a health problem that affects 72.4 million couples worldwide. Infertility can occur as a result of an unhealthy lifestyle, such as a smoking habit. Cigarettes contain nicotine, a toxic compound that can trigger oxidative stress and eventually decrease fertility. Temulawak is a medicinal plant that is rich in antioxidants and anti-inflammatory properties. It has been used as a traditional remedy and is believed to improve fertility. We aim to study the effect of temulawak extract on the morphology, motility, and sperm counts of nicotine-induced mice.
Materials and Methods: A total of 20 mice were divided into four groups (n = 5): control normal (K0); nicotine-induced (K1); and nicotine-induced treated with two different concentrations of temulawak extract, 4 mg/20 g body weight (P1) and 8 mg/20 g body weight (P2). The mice were given nicotine and temulawak for 28 days and then killed by neck dislocation. Both testes and vas deferens were collected for sperm analysis.
Results: Mice treated with nicotine (K1) showed lower sperm counts (p=0.012) and motility (p<0.001) compared to mice in the control normal (K0) but had no difference in morphology. Treatment with temulawak (4 mg/20 g body weight) on nicotine-induced mice (P1) significantly increased sperm motility (p< 0.001). A double dose of temulawak extract (P2) resulted in significantly higher motility and sperm counts than in group K1.
Conclusion: Temulawak treatment (8 mg/20 g body weight) on nicotine-induced mice can significantly improve motility and sperm counts.

Keywords: Nicotine, Temulawak, Spermatozoa, Infertility
Full-Text [PDF 347 kb]   (8120 Downloads) |   |   Full-Text (HTML)  (970 Views)  

✅Temulawak treatment (8 mg/20 g body weight) on nicotine-induced mice can significantly improve motility and sperm counts.


Type of Study: Original Research Article | Subject: Life Science
Received: 2022/01/21 | Accepted: 2022/09/15 | Published: 2023/03/13
References
1. Walsh TJ, Smith JF. Male infertility. In: Smith & Tanagho' s General Urology. 18th ed. New York: McGraw-Hill; 2013. p. 687-719.
2. Agarwal A, Mulgund A, Hamada A, Chyatte MR. A unique view on male infertility around the globe. Reprod Biol Endocrinol. 2015;13(1):1-9. [DOI:10.1186/s12958-015-0032-1] [PMID] [PMCID]
3. Agustina D, Budihastuti UR, Murti B. Biopsychosocial factors on infertility among men in Surakarta, Central Java. Indones J Med. 2018;3(1):14-21. [DOI:10.26911/theijmed.2018.03.01.02]
4. Sharma A. Male infertility; evidences, risk factors, causes, diagnosis and management in human Ann Clin Lab Res. 2017;05(03):1-10. [DOI:10.21767/2386-5180.1000188]
5. Joja OD, Dinu D, Paun D. Psychological aspects of male infertility: An overview. Procedia - Soc Behav Sci. 2015;187:359-63. [DOI:10.1016/j.sbspro.2015.03.067]
6. Kusumawardani N, Tarigan I, Suparmi, Schlotheuber A. Socio-economic, demographic and geographic correlates of cigarette smoking among Indonesian adolescents: Results from the 2013 Indonesian Basic Health Research (RISKESDAS) survey. Glob Health Action. 2018;11(1). [DOI:10.1080/16549716.2018.1467605] [PMID] [PMCID]
7. Nugroho PE, Sudiana K, Djatisoesanto W, Rizaldi F. The effects of nicotine exposure per inhalation to the change of motility and morphology of the rat's sperm. Indones J Urol. 2019;26:179-84. [DOI:10.32421/juri.v26i2.511]
8. Tirtosastro S, Murdiyati AS. Chemical content of tobacco and cigarettes. Bul Planting Tobacco, Fiber, and Miny Ind. 2010;2(1):33-44.
9. Salem NA. Therapeutic effect of curcumin against nicotine-induced reproductive dysfunction in male rats. J Innov Pharm Biol Sci. 2017;4(4):26-31.
10. Sobhani B, Roomiani S, Ahmadi Z, Ashrafizadeh M. Histopathological analysis of testis: Effects of astaxanthin treatment against nicotine toxicity. Iran J Toxicol. 2019;13(1):41-4.
11. Rahmawati I. Effect of nicotine for 1-2 weeks on the number of primary spermatocytes, spermatids in mice (Mus musculus). J Keperawatan Soedirman (The Soedirman J Nursing). 2013;8(3):176-83.
12. Turek PJ. Male reproductive physiology. In: Campbell Walsh Urology. 11th Ed. Philadelphia: Elsevier; 2016. p. 516-537.
13. Halim MRA, Tan MSMZ, Ismail S, Mahmud R. Standardization and phytochemical studies of Curcuma xanthorrhiza Roxb. Int J Pharm Pharm Sci. 2012;4(SUPPL.3):606-10.
14. Rosidi A, Khomsan A, Setiawan B, Riyadi H, Briawan D. Antioxidant potential of temulawak (Curcuma xanthorrhiza Roxb). Pakistan J Nutr. 2016;15(6):556-60. [DOI:10.3923/pjn.2016.556.560]
15. Oon SF, Nallappan M, Tee TT, et al. Xanthorrhizol: A review of its pharmacological activities and anticancer properties. Cancer Cell Int. 2015;15(1):1-15. [DOI:10.1186/s12935-015-0255-4] [PMID] [PMCID]
16. Jalili C, Khani F, Salahshoor MR, Roshankhah S. Protective effect of curcumin against nicotine-induced damage on reproductive parameters in male mice. Int J Morphol. 2014;32(3):844-9. [DOI:10.4067/S0717-95022014000300017]
17. Soleimanzadeh A, Saberivand A. Effect of curcumin on rat sperm morphology after the freeze-thawing process. Vet Res forum an Int Q J. 2013;4(3):185-9.
18. Tvrdá E, Lukáč N, Jambor T, Lukáčová J, Massányi P. Curcumin in male fertility: effects on spermatozoa vitality and oxidative balance. J Microbiol Biotechnol Food Sci. 2015;04(Special issue 2):120-4. [DOI:10.15414/jmbfs.2015.4.special2.120-124]
19. Salahshoor MR, Khazaei M, Jalili C, Keivan M. Crocin improves damage induced by nicotine on a number of reproductive parameters in male mice. Int J Fertil Steril. 2016;10(1):71-8.
20. Adler ID. Comparison of the duration of spermatogenesis between male rodents and humans. Mutat Res - Fundam Mol Mech Mutagen. 1996;352(1-2):169-72. [DOI:10.1016/0027-5107(95)00223-5] [PMID]
21. Cohen A, George O. Animal models of nicotine exposure: Relevance to second-hand smoking, electronic cigarette use, and compulsive smoking. Front Psychiatr. 2013;4:1-21. [DOI:10.3389/fpsyt.2013.00041] [PMID] [PMCID]
Send email to the article author

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

© 2025 CC BY-NC 4.0 | Journal of Advances in Medical and Biomedical Research

Designed & Developed by : Yektaweb