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Volume 32, Issue 155 (November & December 2024)
J Adv Med Biomed Res 2024, 32(155): 466-476 |
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Ethics code: IR.MUK.REC.1400.108
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Feyzi K, Hosseinpanahi A, Derakhshan S. An Outbreak of Carbapenem-Resistant Acinetobacter baumannii in Patients Hospitalized in Intensive Care Units of a Hospital in Western Iran. J Adv Med Biomed Res 2024; 32 (155) :466-476
URL: http://journal.zums.ac.ir/article-1-7542-en.html
URL: http://journal.zums.ac.ir/article-1-7542-en.html
1- Student Research Committee, Kurdistan University of Medical Sciences, Sanandaj, Iran
2- Lung Diseases and Allergy Research Center, Research Institute for Health Development, Kurdistan University of Medical Sciences, Sanandaj, Iran
3- Environmental Health Research Center, Research Institute for Health Development, Kurdistan University of Medical Sciences, Sanandaj, Iran. ,s.derakhshan@muk.ac.ir
2- Lung Diseases and Allergy Research Center, Research Institute for Health Development, Kurdistan University of Medical Sciences, Sanandaj, Iran
3- Environmental Health Research Center, Research Institute for Health Development, Kurdistan University of Medical Sciences, Sanandaj, Iran. ,
Abstract: (45 Views)
Background & Objective: Dissemination of multiple drug-resistant Acinetobacter baumannii in hospitals, particularly in intensive care units (ICUs), may lead to serious outcomes. We aimed to investigate clonal relatedness and the presence of carbapenemase genes in clinical A. baumannii isolated during a hospital outbreak.
Materials & Methods: From October to December 2020, 41 A. baumannii were isolated from patients hospitalized in the ICUs of a university hospital in Kermanshah, western Iran. The sensitivity of isolates to antimicrobial agents was evaluated. The polymerase chain reaction determined the presence of OXA carbapenemases and metallo beta-lactamases. The genetic relationships of isolates were determined using the Enterobacterial repetitive intergenic consensus (ERIC) sequences analysis followed by the Pasteur multilocus sequence typing (MLST) scheme.
Results: All 41 isolates showed resistance to carbapenem meropenem. The blaVIM gene was found in all isolates. Of the 41 isolates, 85.4% carried blaOXA-24 and 48.8% blaOXA-23. No isolate harbored blaOXA-58. Twenty-three isolates (56.1%) had the ISAba1 upstream of the blaOXA-51-like gene. All isolates showed multidrug resistance. ERIC typing revealed the genetic relatedness of isolates. MLST showed that some isolates belonged to sequence type (ST) 415.
Conclusion: The results showed a high level of resistance and clonal spread of A. baumannii in our hospital's ICUs. ST415 might be an emerging A. baumannii lineage carrying carbapenem resistance determinants, and creating awareness regarding preventing this ST may be necessary. Characterization of antimicrobial resistance and determination of genotypes will help track the recurrent episodes of infections with the described A. baumannii isolates.
Materials & Methods: From October to December 2020, 41 A. baumannii were isolated from patients hospitalized in the ICUs of a university hospital in Kermanshah, western Iran. The sensitivity of isolates to antimicrobial agents was evaluated. The polymerase chain reaction determined the presence of OXA carbapenemases and metallo beta-lactamases. The genetic relationships of isolates were determined using the Enterobacterial repetitive intergenic consensus (ERIC) sequences analysis followed by the Pasteur multilocus sequence typing (MLST) scheme.
Results: All 41 isolates showed resistance to carbapenem meropenem. The blaVIM gene was found in all isolates. Of the 41 isolates, 85.4% carried blaOXA-24 and 48.8% blaOXA-23. No isolate harbored blaOXA-58. Twenty-three isolates (56.1%) had the ISAba1 upstream of the blaOXA-51-like gene. All isolates showed multidrug resistance. ERIC typing revealed the genetic relatedness of isolates. MLST showed that some isolates belonged to sequence type (ST) 415.
Conclusion: The results showed a high level of resistance and clonal spread of A. baumannii in our hospital's ICUs. ST415 might be an emerging A. baumannii lineage carrying carbapenem resistance determinants, and creating awareness regarding preventing this ST may be necessary. Characterization of antimicrobial resistance and determination of genotypes will help track the recurrent episodes of infections with the described A. baumannii isolates.
Type of Study: Original Article |
Subject:
Epidemiologic studies
Received: 2024/07/21 | Accepted: 2024/12/1 | Published: 2024/10/10
Received: 2024/07/21 | Accepted: 2024/12/1 | Published: 2024/10/10
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