Volume 33, Issue 160 (September & October 2025)                   J Adv Med Biomed Res 2025, 33(160): 237-242 | Back to browse issues page

Ethics code: IR.ZAUMC.REC.1403.236

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Bokaeian M, Bameri Z, Ansari-Moghaddam A, Vahid A. Antibiotic Resistance Pattern and Prevalence of Class 1, 2, and 3 Integrons in Escherichia coli Strains Isolated from Infected Patients in Zahedan. J Adv Med Biomed Res 2025; 33 (160) :237-242
URL: http://journal.zums.ac.ir/article-1-7663-en.html
Antibiotic Resistance Pattern and Prevalence of Class 1, 2, and 3 Integrons in Escherichia coli Strains Isolated from Infected Patients in Zahedan
Mohammad Bokaeian1 , Zakaria Bameri1 , Alireza Ansari-Moghaddam2 , Amirhosein Vahid *3
1- Infectious and Tropical Diseases Research Center, Zahedan University of Medical Sciences, Zahedan, Iran
2- Health Promotion Research Center, Zahedan University of Medical Sciences, Iran
3- Student Research Committee, Zahedan University of Medical Sciences, Zahedan, Iran , Vahidamirhosein8@gmail.com
Abstract:   (315 Views)

Background and Objectives: Escherichia coli (E. coli) is among the most frequently encountered bacterial pathogens in hospital-acquired infections.‌The only treatment for infections caused by E. coli is the use of various antibiotics. In recent years, E. coli has demonstrated extensive drug resistance, which is partly attributed to the presence of integrons in the genomes of these bacteria. This study aimed to assess the antibiotic resistance profiles and determine the prevalence of class 1, 2, and 3 integrons in Escherichia coli strains isolated from patients infected with the hospital in Zahedan.
Methods: In this cross-sectional study,‌ 200 E. coli separate were collected and, then confirmation of the isolates was performed using biochemical tests and ultimately an antibiogram. The presence of class I, II, and III integron genes was then investigated using specific primers and the PCR method.
Results: Out of 200 E. coli separate,‌170 strains were MDR, with the highest resistance observed to cefazolin (74.5%),‌cotrimoxazole (66.5%),‌ceftriaxone (66.5%),‌and ampicillin (63.5%).‌ The Int1 and Int2 genes were identified in 40% and 12% of the strains,‌respectively; however, the Int3 gene was not found in the studied strains.
Conclusion: The findings of this study reveal a relatively high level of antibiotic resistance among the E. coli isolates, which may be partly attributed to the increased prevalence of integrons. Consequently, restricting the indiscriminate use of antibiotics could help reduce the spread of resistance genes.

Keywords: Integron, Escherichia coli, Antibiotic Resistance
     
Type of Study: Original Research Article | Subject: Clinical Medicine
Received: 2025/05/10 | Accepted: 2025/10/22 | Published: 2025/11/11
References
1. Bonten M, Johnson JR, van den Biggelaar AHJ, Georgalis L, Geurtsen J, de Palacios PI, et al. Epidemiology of Escherichia coli Bacteremia: A Systematic Literature Review. Clin Infect Dis. 2021;72(7):1211-9. [DOI:10.1093/cid/ciaa210] [PMID]
2. Abo-Alella D, Abdelmoniem W, Tantawy E, Asaad A. Biofilm-producing and carbapenems-resistant Escherichia coli nosocomial uropathogens: a cross-sectional study. Int J Microbiol. 2024;27(6):1633-40. [DOI:10.1007/s10123-024-00495-w] [PMID] [PMCID]
3. Nhu NTK, Phan MD, Hancock SJ, Peters KM, Alvarez-Fraga L, Forde BM, et al. High-risk Escherichia coli clones that cause neonatal meningitis and association with recrudescent infection. Elife. 2024(12):RP91853. [DOI:10.7554/eLife.91853] [PMID] [PMCID]
4. Hall GS. Bailey & Scott's diagnostic microbiology. 13th ed: American Society for Clinical Pathology; 2013.
5. Jaybhaye A, Deb M. Pathogenesis of Escherichia coli: A clinical findings. J Pharm Res Int Dec. 2021;27(60B):3185-91. [DOI:10.9734/jpri/2021/v33i60B34995]
6. Kousha A, Kavakebi N, Alikhah F. Reporting problems of National Nosocomial Infections Surveillance System (NNIS) in Tabriz hospitals. 2016:20163114118.
7. Diekema DJ, Hsueh PR, Mendes RE, Pfaller MA, Rolston KV, Sader HS, et al. The Microbiology of Bloodstream Infection: 20-Year Trends from the SENTRY Antimicrobial Surveillance Program. Antimicrob Agents Chemother. 2019;63(7):e00355. [DOI:10.1128/AAC.00355-19] [PMID] [PMCID]
8. Bimanand L, Pakzad I, Sayehmiri K, Yasemi M, Sayehmiri F, Peyman H, et al. Prevalence of Urinary Tract Infection and Associated Microorganisms in Iran; A meta-Analysis Study. Res J Pharm Biol Chem Sci. 2017;8(1):1255-62.
9. Pormohammad A, Nasiri MJ, Azimi T. Prevalence of antibiotic resistance in Escherichia coli strains simultaneously isolated from humans, animals, food, and the environment: a systematic review and meta-analysis. Infect Drug Resist. 2019;12:1181-97. [DOI:10.2147/IDR.S201324] [PMID] [PMCID]
10. Sabbagh P, Rajabnia M, Maali A, Ferdosi-Shahandashti E. Integron and its role in antimicrobial resistance: A literature review on some bacterial pathogens. Iran J Basic Med Sci. 2021;24(2):136-42.
11. Rahman MM, Hossain MMK, Rubaya R, Halder J, Karim ME, Bhuiya AA, et al. Association of Antibiotic Resistance Traits in Uropathogenic Escherichia coli (UPEC) Isolates. Can J Infect Dis Med Microbiol. 2022;2022:4251486. [DOI:10.1155/2022/4251486] [PMID] [PMCID]
12. Kumar G, Balakrishna K, Mukhopadhyay C, Kalwaje Eshwara V. Comparison of integron mediated antimicrobial resistance in clinical isolates of Escherichia coli from urinary and bacteremic sources. BMC Microbiol. 2024;24(102):1-6. [DOI:10.1186/s12866-024-03250-3] [PMID] [PMCID]
13. Pormohammad A, Pouriran R, Azimi H, Goudarzi M. Prevalence of integron classes in Gram-negative clinical isolated bacteria in Iran: a systematic review and meta-analysis. Iran J Basic Med Sci. 2019;22(2):118-27.
14. Souque C, Escudero JA, MacLean RC. Integron activity accelerates the evolution of antibiotic resistance. Elife. 2021(10):e62474. [DOI:10.7554/eLife.62474] [PMID] [PMCID]
15. Barzegar S, Arzanlou M, Teimourpour A, Esmaelizad M, Yousefipour M, MohammadShahi J, et al. Prevalence of the Integrons and ESBL Genes in Multidrug-Resistant Strains of Escherichia coli Isolated from Urinary Tract Infections, Ardabil, Iran. Iran J Med Microbiol. 2022;16(1):56-65. [DOI:10.30699/ijmm.16.1.56]
16. Ibrahim DR, Dodd CER, Stekel DJ, Meshioye RT, Diggle M, Lister M, et al. Multidrug-Resistant ESBL-Producing E. coli in Clinical Samples from the UK. Antibiotics (Basel). 2023;12(1):169. [DOI:10.3390/antibiotics12010169] [PMID] [PMCID]
17. Yu D, Banting G, Neumann NF. A review of the taxonomy, genetics, and biology of the genus Escherichia and the type species Escherichia coli. Can J Microbiol. 2021;67(8):553-71. [DOI:10.1139/cjm-2020-0508] [PMID]
18. Clinical and Laboratory Standards Institute (CLSI). Performance Standards for Antimicrobial Susceptibility Testing, 33rd edition. 2024.
19. Kargar M, Mohammadalipour Z, Doosti A, Lorzadeh S, Japoni-Nejad A. High Prevalence of Class 1 to 3 Integrons Among Multidrug-Resistant Diarrheagenic Escherichia coli in Southwest of Iran. Osong Public Health Res Perspect. 2014;5(4):193-8. [DOI:10.1016/j.phrp.2014.06.003] [PMID] [PMCID]
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