Volume 33, Issue 162 (Special Issue 2025)                   J Adv Med Biomed Res 2025, 33(162): 148-155 | Back to browse issues page

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Al-Qaysi D B A, Mousa Z Q, Sukkar Z J. Inhibitory Effect of Syzygium aromaticum Extract on the Growth of Pseudomonas aeruginosa Clinical Isolates Associated with Urinary Tract Infections. J Adv Med Biomed Res 2025; 33 (162) :148-155
URL: http://journal.zums.ac.ir/article-1-7866-en.html
Inhibitory Effect of Syzygium aromaticum Extract on the Growth of Pseudomonas aeruginosa Clinical Isolates Associated with Urinary Tract Infections
Diana Basim Abdulhameed Al-Qaysi *1 , Zahraa Qasim Mousa2 , Zainab Jabbar Sukkar3
1- Department of Clinical Laboratories Sciences, College of Pharmacy, University of Misan, Maysan, Iraq , diana_basim@uomisan.edu.iq
2- Department of Biology, College of Science, University of Misan, Maysan, Iraq & General Directorate for Education in the province of Maysan, Ministry of Education, Maysan, Iraq
3- Department of Clinical Laboratories Sciences, College of Pharmacy, University of Misan, Maysan, Iraq
Abstract:   (187 Views)

Background & Objective: Syzygium aromaticum is the scientific name for the aromatic flower buds of an Indonesian native tree from the Myrtaceae family.  Cloves contain bioactive compounds such as caryophyllene, eugenol, and acetyl eugenol, which have been shown to possess various physiological properties, including antioxidant, antibacterial, analgesic, anti-inflammatory, anticancer, and anesthetic effects. The present study aims to examine the antibacterial effects of S. aromaticum extracts prepared using different solvents (methanol, ethanol, petroleum ether, n-hexane, and chloroform) against P. aeruginosa.
 Materials & Methods: A total of 93 urine samples were collected from inpatients at Al-Sadder Hospital in Maysan, Iraq. The study specifically targeted urinary tract infections (UTIs) caused by Pseudomonas aeruginosa. The antibacterial activity of various S. aromaticum extracts (prepared using methanol, ethanol, petroleum ether, hexane, and chloroform) was evaluated against the isolates using the agar well diffusion method. The extracts were tested at concentrations of 62.5, 125, and 500 µg/mL and compared with a standard antibiotic. This investigation aimed to verify the antimicrobial potential of S. aromaticum against P. aeruginosa, with the findings highlighting its promise for medicinal applications.
Results:  Phytochemical screening revealed the presence of tannins, flavonoids, terpenoids, alkaloids, phlobatannins, and saponins in the extracts. Among the tested extracts, the ethanol extract showed the lowest activity against P. aeruginosa, producing an inhibition zone of 24 mm at 500 µg/mL. The methanol extract exhibited the highest antibacterial activity, with an inhibition zone of 40 mm at 500 µg/mL. The P. aeruginosa isolates were sensitive to Gentamicin, Neomycin, Enrofloxacin, Imipenem, and Meropenem but resistant to Cefadroxil, Cephalexin, Ceftriaxone, Ciprofloxacin, and Sulfamethoxazole.
Conclusion:  The results demonstrate notable antibacterial activity of S. aromaticum particularly its methanolic extract against P. aeruginosa. This potent activity is attributed to its diverse phytochemical composition. Overall, S. aromaticum shows potential as a natural source for developing novel antimicrobial agents capable of combating multidrug-resistant infections.

Keywords: S. aromaticum, Phytochemical Composition, Pseudomonas aeruginosa, Urinary Tract Infections
Full-Text [PDF 470 kb]   (7 Downloads)    
Type of Study: Original Research Article | Subject: Pharmacology
Received: 2025/09/26 | Accepted: 2025/11/18 | Published: 2025/12/29
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