Background and Objective: Peptide as an agent in manufacturing new drugs has recently been taken into consideration. Brevinin-2R is a nano peptide with a wide range of biological actions. Thus, the current study was designed in order to evaluate the in vitro effects of Brevinin-2R on oral squamous cell carcinoma cells in comparison to normal mice fibroblast cells.

Materials and Methods: In this study, oral squamous cell carcinoma cells (KB) and normal mice fibroblast cells (L929) were cultured and  treated with different concentrations of Brevinin-2R (0.1-30 µg/ml) for 24, 48 and 72 hours. Then, cell viability was calculated by MTT method. Data were fed to SPSS software (version 20) via which One Way ANOVA and Tukey tests was calculated for the collected data.

Results: The half-maximal inhibitory concentration (IC50) of Brevinin-2R for KB cells and L929 cells were 15.48 and 24.14 µg/ml respectively, demonstrating that higher concentration of Brevinin-2R is necessary in order to reach IC50 concentration for L929 cells (about 1.5 times).

Conclusion: Results point out that Brevinin-2R peptide is more effective on oral squamous cell carcinoma cells compared to normal mice fibroblast cells (L929).