Volume 26, Issue 117 (9-2018)                   J Adv Med Biomed Res 2018, 26(117): 96-108 | Back to browse issues page

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Ghavami M B, Khoeini S, Faghih Zadeh S. Evaluation of Ziziphora tenuior Essential Oil Repellency Effect Against Blood Feeding of Anopheles maculipennis in Zanjan District. J Adv Med Biomed Res 2018; 26 (117) :96-108
URL: http://journal.zums.ac.ir/article-1-5410-en.html
Evaluation of Ziziphora tenuior Essential Oil Repellency Effect Against Blood Feeding of Anopheles maculipennis in Zanjan District
Mohammad Bagher Ghavami * 1, Sakineh Khoeini2 , Soghrat Faghih Zadeh3
1- Dept. of Medical Entomology & Vector Control, Faculty of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran , Ghavami@zums.ac.ir
2- Dept. of Medical Entomology & Vector Control, Faculty of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran
3- Dept of. Statistics and Social Medicine, Faculty of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran
Abstract:   (154727 Views)

Background and Objective: Anopheles maculipennis is one of the most important species of mosquito that can cause serious public health problems worldwide and applying insecticides and synthetic repellents are the current methods used for its control. The continuous application of these chemicals increases the probability of resistance, reduces insecticide efficiency and causes environmental problems. Therefore, introducing alternative substances (especially natural repellents) to control programs is necessary. The aim of this study was to compare the repellency effect of Ziziphoratenuior essential oil (EO) with DEET.
Materials and Methods: Ziziphoratenuior was collected from Zanjan and its EO was extracted from dried leaves via a hydro-distillation process. The chief components of the essential oil were identified through the GC-mass method and serial dilutions of the essential oil and DEET were prepared. Adult An. Maculipennis’ were collected and kept in a laboratory. Contact bioassays were conducted by 9 volunteers, through a single-blind study where their arms were impregnated with repellent solutions. Non-blood fed female mosquitoes were exposed to different doses of repellents. The effective doses of 50% (ED50) and 90% (ED90) and duration of repellency were determined for each repellent.
Results: Thymol, geraniol and carvacrol are the chief components of Ziziphora EO and form 36.2%, 11.16% and 4.9% of it, respectively. The ED50 and ED90 for this EO against An. Maculipennis were 1.7 and 3.67 mg/cm2, respectively. A 30% Ziziphorasolution prevented biting for 240 minutes. The response of An. Maculipennis to Ziziphora EO in high doses was similar to that of DEET, however, this response differed in doses lower than 1 mg/cm2.
Conclusion: Ziziphora essential oil 30% solution has similar repellency effect to DEET and can prevent biting of An. Maculipennis for 240 minutes. Synergists could be added to increase its effectiveness and electrophysiologic studies could determine the mechanism of action of thymol and carvacrol at molecular levels.

Keywords: Anopheles maculipennis, Ziziphora essential oil, Repellents, DEET, Ziziphoratenuoir
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Type of Study: Original Article |
Received: 2018/11/13 | Accepted: 2018/11/13 | Published: 2018/11/13
References
1. Service MW (2012). Medical Entomology for Students,5th Ed, Cambridge Academic Press. 2012: 34-51. [DOI:10.1017/CBO9781139002967]
2. Ghavami MB, Ladoni H. Funa and frequency of mosquito species in Zanjan province. J Zanjan Univ Med Sci. 2005; 13: 46-54. [in Persian].
3. Ghavami MB. Estimation and comparison of Anopheles maculipennis s.l. (Diptera: Culicidae) survival rates with light-trap and indoor resting data. Iranian J Publ Health. 2005; 34: 48-57.
4. World Health Organization, A global brief on vector-born diseases. 2014; 1: 30-38.
5. Patel EK, Gupta A, Oswal RJ. A review on: mosquito repellent methods. IJPCBS. 2012; 2: 310-17.
6. Senejouxa F, Girarda C, Kerramb P, et al. Mechanisms of vasorelaxation induced by Ziziphora clinopodioides Lam. (Lamiaceae) extract in rat thoracic aorta. J Ethnopharmacol. 2010; 132: 268-73. [DOI:10.1016/j.jep.2010.08.028] [PMID]
7. Azadmehr A, Mosalla S, Hajiaghaee R, Shahnazi M. Immunomodulatory effects of Ziziphora tenuior L. extract on the dendritic cells. DARU J Pharm Sci. 2014; 22: 1-6. [DOI:10.1186/s40199-014-0063-8] [PMID] [PMCID]
8. Celik C, Tutar U, Karaman I, Hepokur C, Atas M. Evaluation of the antibiofilm and antimicrobial properties of Ziziphora tenuior L. Essential oil against multidrug-resistant Acinetobacter baumanni. Int J Pharmacol. 2016; 12: 28-3. [DOI:10.3923/ijp.2016.28.35]
9. Abu-Darwish MS, Cabral C, Goncalves MJ, et al. Ziziphora tenuior L. essential oil from dana biosphere reserve (Southern Jordan); chemical characterization and assessment of biological activities. J Ethnopharmacol. 2016; 24: 963-70. [DOI:10.1016/j.jep.2016.10.076] [PMID]
10. Verdian-Rivi M. Effect of the essential oil composition and biological activity of Ziziphora clinopodiodes Lam. on the against Anopheles stephensi and Culex pipiens larva from Iran. Saudi J Biol Sci. 2008: 15: 185-88.
11. Ghavami MB, Poorrastgoo F, Taghiloo B, Mohammad J. Repellency effect of essential oils of some native plants and synthetic repellents against human flea, Pulex irritans (Siphonaptera: Pulicidae) J Arthropod Borne Dis. 2017; 11: 105-15. [DOI:10.1016/j.pestbp.2018.03.004] [PMID]
12. Choochote W, Chaithong U, Kamsuk K, et al. Repellent activity of selected essential oils against Aedes aegypti. Fitoterapia. 2007; 78: 359-64. [DOI:10.1016/j.fitote.2007.02.006] [PMID]
13. Kumar S, Wahab N, Warikoo R. Bioefficacy of Mentha piperita essential oil against dengue fever mosquito Aedes aegypti L. Asian Pac J Trop Biomed. 2011; 1: 85-88. [DOI:10.1016/S2221-1691(11)60001-4]
14. Moore SJ, Darling ST, Sihuincha M, Padilla N, Devine GJ. A low-cost repellent for malaria vectors in the Americas: results of two field trials in Guatemala and Peru. Malaria J. 2007; 6:101-6. [DOI:10.1186/1475-2875-6-101] [PMID] [PMCID]
15. Trongtokit Y, Rrongsriyam Y, Komalamisra N Apiwathnasorn CH. Comparative repellency of 38 essential oils against mosquito bites. Phytother Res. 2005; 19: 303-9. [DOI:10.1002/ptr.1637] [PMID]
16. Ansari MA, Vasudevan P, Tandon M,Razdan RK. Larvicidal and mosquito repellent action of peppermint (Mentha piperita) oil. Bioresour Technol. 2000; 71: 267-71. [DOI:10.1016/S0960-8524(99)00079-6]
17. Nzira L, Per M, Peter F, Claus B. Lippia javanica (Burm F) Spreng: its general constituents and bioactivity on mosquitoes. Trop Biomed. 2009; 26: 85-91.
18. Tunon H, Thorsell W, Mikiver A, Malander I. Arthropod repellency, especially tick (Ixodes ricinus), exerted by extract from Artemisia abrotanum and essential oil from flowers of Dianthus caryophyllum.Fitoterapia 2006; 77: 257-61. [DOI:10.1016/j.fitote.2006.02.009] [PMID]
19. Fradin MS, Day JF. Comparative efficacy of insect repellents against mosquito bites. South Pacific Underwater Medicine Society (SPUMS) Journal. 2003; 33: 103-4.
20. Pirmohammadi M, Shayeghi M, Vatandoost H, et al. Chemical composition and repellent activity of Achillea vermiculata and Satureja hortensis against Anopheles stephensi. J Arthropod-Borne Dis. 2016; 10:201-10.
21. Sritabutra D, Soonwera M, Waltanachanobon S, Poungjai S. Evaluation of herbal essential oil as repellents against Aedes aegypti (L.) and Anopheles dirus Peyton & Harrion. Asian Pac J Trop Biomed. 2011; 1: 124-8. [DOI:10.1016/S2221-1691(11)60138-X]
22. Govindarajan M. Larvicidal and repellent properties of some essential oils against Culex tritaeniorhynchus Giles and Anopheles subpictus Grassi (Diptera: Culicidae). Asian Pac J Trop Biomed. 2011; 4: 106-11. [DOI:10.1016/S1995-7645(11)60047-3]
23. Siriporn P, Mayura S. The effects of herbal essential oils on the oviposition-deterrent and ovicidal activities of Aedes aegypti (Linn.), Anopheles dirus (Peyton and Harrison) and Culex quinquefasciatus (Say). Asian Pac J Trop Biomed. 2012; 29: 138-50.
24. Tavassoli M, Shayeghi M, Abai MR,et al. Repellency effects of essential oils of myrtle (Myrtus communis), Marigold (Calendula officinalis) compared with DEET against Anopheles stephensi on human volunteers. Iran J Arthropod Borne Dis. 2011; 5: 10-22.
25. Omolo MO, Okinyo D, Ndiege IO, Lwande W, Hassanali A. Repellency of essential oils of some Kenyan plants against Anopheles gambiae. Phytochemistry. 2004; 65: 2797-802. [DOI:10.1016/j.phytochem.2004.08.035] [PMID]
26. Park BS, Choi WS, Kim JH, Kim KH. Monoterpenes from thyme (Thymus vulgaris) as potential mosquito repellents. J Am Mosq Control Assoc. 2005; 21: 80-3. [DOI:10.2987/8756-971X(2005)21[80:MFTTVA]2.0.CO;2]
27. Rehman JU, Ali A, Khan IA. Plant based products: Use and development as repellents against mosquitoes: Fitoterapia. 2014; 95: 65-74. [DOI:10.1016/j.fitote.2014.03.002] [PMID]
28. Auysawasdi N, Chuntranuluck S, Phasomkusolsil S, Keeratinijakal V. Improving the effectiveness of three essential oils against Aedes aegypti (Linn.) and Anopheles dirus (Peyton and Harrison). Parasitol Res. 2016; 115: 99-106. [DOI:10.1007/s00436-015-4725-3] [PMID]
29. Lutgen P. Mosquitocidal and repellent properties of plant extracts.Malaria World. 2015; 14: 27.
30. Popovic A, Sucur J, Orcic D, Strbac P. Effects of essential oil formulations on the adult insect Tribolium castaneum (Herbst) (Col: Tenebrionidae). J Central Europe Agriculture. 2013; 14: 181-93. [DOI:10.5513/JCEA01/14.2.1246]
31. Pavela R, Benelli G. Ethnobotanical knowledge on botanical repellents employedin the African region against mosquito vectors. Experimental Parasitology. 2016; 167: 103-8. [DOI:10.1016/j.exppara.2016.05.010] [PMID]
32. Khalequzzaman M, Ara H, Zohura F, Nahar J. Toxic, repellent and attractant properties of some insecticides towards the housefly (Musca domestica L.). On; ine. J Biol Sci. 2002; 2: 672-76. [DOI:10.3923/jbs.2002.672.676]
33. Maia MF, Moore S. Plant-based insect repellents: a review of their efficacy, development and testing. Malaria J. 2011; 10: 11-25. [DOI:10.1186/1475-2875-10-S1-S11] [PMID] [PMCID]
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