Volume 27, Issue 120 (January & February 2019)                   J Adv Med Biomed Res 2019, 27(120): 1-7 | Back to browse issues page

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Shokoohizadeh L, Saniee M, Mirzaee M, Taheri M. Mutations in gyrA and parC Genes in Quinolone-Resistant Klebsiella pneumoniae Isolates from Borujerd Hospitals. J Adv Med Biomed Res 2019; 27 (120) :1-7
URL: http://journal.zums.ac.ir/article-1-5763-en.html
Mutations in gyrA and parC Genes in Quinolone-Resistant Klebsiella pneumoniae Isolates from Borujerd Hospitals
Leili Shokoohizadeh1 , Mehrangiz Saniee2 , Mohsen Mirzaee2 , Mohammad Taheri * 3
1- Dept. of Medical Microbiology, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran
2- Dept. of Medical Laboratory Sciences, Borujerd Branch, Islamic Azad University, Borujerd, Iran
3- Dept. of Medical Microbiology, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran , motaheri360@gmail.com
Abstract:   (149705 Views)

Background and Objective: Quinolones are the antibiotics used to treat infections. Several reports have indicated the increased resistance to quinolone in K. pneumoniae strains all over the world. The aim of this study is to investigate amino acid substitutions in GyrA and ParC proteins among quinolone-resistant isolates of K. pneumoniae in Borujerd )west of Iran( hospitals.
Materials and Methods: Totally, 100 isolates of K. pneumoniae were collected. After validating the isolates by conventional laboratory methods, an antimicrobial susceptibility test was carried out for some antibiotics from the quinolones family. Quinolone resistance and Minimum Inhibitory Concentration (MIC) of Ciprofloxacin were detected by disk diffusion and E-test methods, respectively. The amplification of gyrA and parC genes in quinolone-resistant strains was performed by PCR using specific primers. PCR products were sequenced in order to detect the mutations in gyrA and parC genes.
Results: Generally, 38% of all the collected isolates were resistant to Nalidixic acid and Ciprofloxacin, 18% were resistant to Ofloxacin, and 15% were resistant to Norfloxacin. Concurrent resistance to Nalidixic acid, Ciprofloxacin, Ofloxacin, and Norfloxacin was determined in 15% of the cases. In 86% (n=20) of Ciprofloxacin-resistant strains, MIC was measured 128 μg/mL. The mutation rate was 40% (n=9) in gyrA gene in quinolone-resistant strains and 35% (n=8) in parC gene.
Conclusion: Briefly, our research findings reveal that relatively high resistance to quinolones as well as fluoroquinolone was observed among K. pneumoniae isolates in Brojurd hospitals. It is likely that mutation occurrence in certain positions of gyrA and parC genes has a significant effect on developing high-level resistance to quinolone in K. pneumoniae.

Keywords: Klebsiella pneumoniae, gyrA and parC mutations, Quinolone resistance
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✅ Briefly, our research findings reveal that a relatively high resistance to quinolones as well as fluoroquinolone was observed among K. pneumoniae isolates in Brojurd hospitals. It is likely that mutation occurrence in certain positions of gyrA and parC genes has a significant effect on developing high-level resistance to quinolone in K. pneumoniae.


Type of Study: Original Article | Subject: Medical Biology
Received: 2019/09/3 | Accepted: 2019/09/3 | Published: 2019/09/3
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