Background and objectives: Due to the benefits of regular physical activity and prevalence of sedentary of physical activity among females and the potential of health education theories and models in changing behaviors, this study was designed to assess the effect of physical education (II) based on BASNEF Model on female students’ regular physical activity of Isfahan university of medical sciences. Materials and Methods: All female students who registered in physical education Π classes in the first semester of academic year 2007-2008, participated in this experimental intervention program (N=120). These students were from six classes and randomly divided into two groups: experimental group (3 classes) and control group (3 classes). The data was collected using a developed questionnaire based on BASNEF Model (contain Belief, Attitude, Subjective Norm, Enabling Factors components) as well as international physical activity questionnaire (IPAQ). The experimental group participated in 2 months ordinary physical education program based on BASNEF Model. The control group had 2 months ordinary educational program. The data was collected in pre and post-test periods and then 2 and 4 months follow-up and analyzed using SPSS software. Results: After intervention, the mean scores of BASNEF components were significantly high in experimental group in comparison to the control group. Also the experimental groups’ participation in regular physical activity was significantly greater than the control group after 2 and 4 months prior to the interventional program. Conclusion: Developing physical education courses based on BASNEF model can improve regular physical activity of female students.

Background and Objectives: In this study, capability of Vero cells for growth on FibraCel disks were compared on 3 kinds of microcarriers including Cytodex-1, Cutodex-3, and Sigma Solohill in 500 ml Spinner flasks in both serum contained medium (DMEM+10% Fetal Calf Serum, FCS) and serum-free medium (VP-SFM). The propagation of fixed PV (Pasteur Virus) strain of rabies in Vero cells, for production of rabies vaccine, grown under the above conditions were studied and compared. Materials and Methods: Stepwise perfusion mode in growth phase and batch mode were applied in the virus production step by the use of M199 +0.2% Bovine Serum Albumin (BSA) and VP-SFM as serum containing and serum-free media, respectively. The available surface area provided by the carriers, and primary cell density in the experiments were assumed the same (about 12,000 cm2 and 12,500 cells/cm2, respectively). Results: The highest cell density was achieved on FibraCel disks in DMEM equal to 7.1 ±0.7×106 cells /ml on day 9, while the lowest cell density was obtianed on Cytodex-3 in VP-SFM equal to 2.91 ±0.2×106 cells/ml. The highest virus titer (55.18 ±4.4×106 Fluorescent Focus Unit,FFU/ml) was gained in VP-SFM containing FibraCel disks, and the lowest titer (3.66 ±0.4 ×106 FFU/ml) was resulted on Cytodex-3 in M199. Conclusion: In these experiments, FibraCel disks supported the growth of Vero cells better than the microcarriers, and the use of DMEM for propagation of Vero cells and VP-SFM for proliferation of rabies virus showed better results. The experimental vaccine prepared by collected virus from VP-SFM has an acceptable potency of 2.75 IU. Based on these results and to the relative ease for making FibraCel disks, we recommend the use of this carrier for propagation of Vero cells and production of rabies virus.

Background and Objective: Celery (Apium graveolens) is a plant from Apiaceae family with high nutritional and medicinal use.This plant has many phytoestrogens that can affect the pituitary-gonadal axis. The purpose of this study was to investigate the effect of hydro-alcoholic extract of celery leaves on serum level of testosterone, FSH and LH in male rats. Materials and Methods: In this experimental study, thirty-two male wistar rats were randomly divided into four groups of eight rats each. The control group did not receive anything. The sham group received distilled water (as a solvent), and the experimental groups received doses of 200 and 300 mg/kg/ BW of hydro-alcoholic extract of celery leaves for 20 days. The extract was gavaged once a day. One day after the last gavage, the rats were anaesthetized and blood samples were collected from the heart and then serum levels of testosterone, LH and FSH were measured. The data were analyzed using SPSS software and ANOVA test. Results: Concentration of LH in the treatment group with doses of 200 mg/kg (0.39±0.02 mIU/dl) reduced in comparison with control (0.67±0.01 mIU/dl) and sham (0.73±0.02 mIU/dl) groups (P<0.05). No significant difference was observed in serum level of testosterone and FSH hormones in comparison with control group (P>0.05). Conclusion: The results indicated that the administration of 200 mg/kg doses of celery extract causes a significant reduction in serum LH concentration, but it has no effect on ganadotropin and testosterone hormones in highest doses used in this study. This finding may be due to the presence of flavonoid and antioxidant proporties of celery.