Showing 21 results for sadeghi
Leyla Kasraeian, Ali Sadeghi Hsanabadi,
Volume 8, Issue 32 (9-2000)
Abstract
Family planning programs in Islamic Republic of Iran is faced with limited resources. So the evaluation of family planning programs, in order to determine how much we have reached to our targets, is so important.The easiest way for measuring the results of these activities is by using a couple year of protection.Method and Material: This study is conducted in order to clarify the couple-year of protection of three methods of contraception: condom, tubectomy, vasectomy.300 condom users, 290 cases of tubectomey and 290 cases that vasectome was done for their husbands were selected by simple random sampling from the health centers in Shiraz. They were asked about mean coital frequency per month in condom users and asked about age of women during using permanent contraceptive method, and then CYP was calculated.Results: Converting factors were 0.012, 9.82 and 10.76 in order for condom, tubectomy, and vasectomy.Mean live children number was 4.8 + 0.52 for vasectomy and 5.21 + 0.76 for tubectomy. Educational level of cases of vasectomy was statistically significant in comparison to tubectomy (P<0.05).Conclusion: Converting factors of permanent methods are less than Wishik coefficient (12) and these methods have the least protection.With regard to the importance of age of using these methods on providing protection, these methods must be done of younger age. Converting factor of condom is more than wishik coefficient.
Niloofar Motamed, Seyed Alireza Ayatollahi, Najaf Zare, Ali Sadeghi Hasanabadi,
Volume 10, Issue 38 (3-2002)
Abstract
Background: Measuring perception of the population about their health in order to evaluate the usefulness of health care is important. To ascertain the reliability and validity of the Persian version of SF-36 health questionnaire, this study was conducted on the staff of Shiraz Medical School in 2001.
Materials and Methods: In this descriptive study, we used alpha Cronbach's coefficient for reliability, one way ANOV A, Spearman's correlation coefficient, and linear regression for validity.
Results: Of some 134 questionnaires distributed, 112 ones (83.6%) were sent back. The responses rate was 83.6%. SF-36 was internally consistent as Cronbach's-Alpha coefficient was 0.87 which was more than the least recommended of 0.85. The construct validity of SF-36 was acceptable as the scores of physical component score and physical functioning decreased significantly with increasing age. Also, these two scores were less in married people. The correlation between physical and mental component scores and eight scales were acceptable.
Conclusion: The reliability and validity of the translated SF-36 were acceptably high. The translated SF-36 questionnaire is recommended for the assessment of population's health status due to its speed and simpleness. However, the choices regarding physical and psychological health seem to need reform. In the end, more studies with greater sample size to uncover other facts are strongly recommended.
Mehran Mohammadian Fazli, Gholamreza Sadeghi,
Volume 11, Issue 43 (Jun 2003)
Abstract
Background: Zanjan city with population of about 300,000 is supplied with groundwater resources for drinking water. Now, Zanjan sewage is disposed to cesspools. On the other hand, precipitation has decreased during the past decade. Because of the importance of the safe drinking water, this study was conducted to determine some chemicals and microbial characteristics in drinking water in 1999-2000.
Materials and Methods: This descriptive study was performed on total drinking water resources (wells) in Zanjan. During four seasons, 280 samples were collected and nitrate, nitrite and chloride concentrations were measureed by spectrophotometry and argantometric titration. Total coliforms and fecal coliforms were detected according to the approved methods. The data were analysed by Kroscal Wallis method.
Results: In this research, 36 drinking water wells were divided into two groups: 22 wells in the city and 14 wells out of the city. Mean concentrations of nitrate, nitrite and chloride in the wells inside the city, in comparison with the ones' outside, were statistically higher (p<0.0003, p<0.003, p<0.0003) respectively. In some wells inside the city, the nitrate was greater than of its standard limit, but the nitrite and chloride levels were less than the standard limits in all the wells. All the wells had desirable microbiological quality.
Conclusion: Nitrate and nitrite concentrations in some drinking water wells were undesirable and some of the wells were exposed to the contamination. Thus, it is necessary to determine the nitrate and nitrite concentrations in drinking water distribution system.
Mahnaz Sadeghi Shabestari, Hamideh Hosseinpour Feizi,
Volume 14, Issue 54 (Mar 2006)
Abstract
Adverse reactions induced by BCG vaccination are rare and appear either in the form of lymphadenitis or osteitis. One of the rarest complications of the vaccine is disseminated mycobacterial infection which mostly occurs in infants with immune deficiency. In this paper a case of disseminated BCG infection is reported in a four-month-old infant suffering from severe combined immunodeficiency (SCID). Plain radiographs showed multiple osteolytic lesions in skull and extremities abdominal sonography revealed multiple nodules in the liver and spleen in thoracic radiography disseminated interstitial infiltration was observed, and gastric tub age culture tested positive for Mycobacterium bovis.
Rahmatoalleh Parandin, Dr Hamidreza Sadeghipour Rodsari, Dr Sirous Shamili, Dr Hamidreza Ghasempour,
Volume 16, Issue 65 (12-2008)
Abstract
Background and Objective: Evaluation of the importance and the effects of plant derived drugs on fertility of laboratory animals have long been recognized. In this research the effects of administration of aqueous extract of Boswellia thurifera on fertility indices, body weight and reproductive organs weight was evaluated in male rats. Materials and Methods: Eighteen rats were randomly divided into 3 groups, control, group A and group B. Each group comprises of six rats. Animals in control group received 1 ml of distilled water (vehicle) and test groups (A and B) received graded doses of 200 and 400 mg/kg body weight of aqueous extract of Boswellia thurifera respectively on daily basis for 60 days. At the end of 60 days of treatment period, fertility indices such as body weight, reproductive organs weight, sperm motility and count, fertility rate, epididymal sperm reserve (ESR), daily sperm production (DSP) and blood testosterone concentration were measured. Results: There was a significant increase in the reproductive organs and body weight, sperm motility and count, ESR, DSP, blood testosterone concentration and fertility rate especially in the group which received the higher doses of aqueous extract of Boswellia thurifera. Conclusion: The results of this study showed that aqueous extract of Boswellia thurifera could increase fertility rate in male rats. Key words: Boswellia thurifera, Fertility, Male rats
Iraj Moghaddami, Dr Mino Iikhanipoor, Dr Godarz Sadeghi-Hashjin,
Volume 17, Issue 69 (12-2009)
Abstract
Background and objectives: Non steroidal anti inflammatory drugs (NSAIDs) are widely used to reduce inflammation, pain and fever. The present study was organized to induce an experimental inflammation in an animal model, using a putative biological oxidant, Peroxynitrite (ONOO-) and to study the effects of Flunixin meglumine and Ketoprofen on the pressure-induced pain. Materials and Methods: For this purpose, 24 male guinea pigs were divided into 4 groups each consisting of 6 animals. Three groups (groups 2, 3 and4) were injected Peroxynitrite and one group (the first: control) received physiological salt solution subcutaneously in the paw. After induction of a local inflammatory response, Flunixin meglumine (1mg/kg) and Ketoprofen (2 mg/kg) were injected to the second and third groups, 5 times with 12h intervals. The first and the fourth groups were injected saline solution in the same manner. Pressure analgesiometry was performed before and 1 hour after injections. Result: The animals in all 3 groups treated with Peroxynitrite demonstrated an increased sensitivity to painful pressure (P<0.05). Both NSAIDs decreased the pain sensation dramatically after the 1st and the 2nd injections but, not after the 3rd, 4th and the 5th injections. Conclusion: The study suggested that NSAIDs may be helpful in reducing pressure-induced pain in animal model in early hours of the treatment, whereas the effect subsides over time and ends up after a few days. This effect may be of importance in humans who receive these kinds of drugs for a long period as they may not be effective in reducing pain after a while.
Amir Vahedian Azimi, Mostafa Sadeghi, Ali Movafegh, Rahim Sorouri Zanjani, Davoud Hasani, Ar Salehmoghaddam, Mohammad Bagher Kashshsfi,
Volume 20, Issue 78 (3-2012)
Abstract
Background and Objectives: Stress and stressful situations can be a prelude to fatal diseases such as cardiovascular diseases and hypertension. The aim of this study was to investigate the relationship between perceived stress with five major characteristics of the heart disease in patients with myocardial infarction. Materials and Methods: In this cross sectional descriptive-analytical study that was conducted from May 2005 till October 2009, a total of 3,200 patients with myocardial infarction, from cardiovascular care unit of Gha'em and Imam Reza hospital, Mashhad, were randomly selected. A demographic questionnaire, an instrument for recording laboratory and electrocardiograph finding, and the Perceived Stress Scale were used for data collection. Results: The results of this study show that while 35% of all cases suffer from moderate stress, 65% percent of them suffer from high level of stress. The level of perceived stress in different categories of variables such as gender, educational level, hypertension, history of hypertension, depression, cigarette smoking, exercising, job, level of incoming, location of living, and family history of cardiovascular disease was significantly different from each other. Conclusion: Considering the high levels of stress among patients with myocardial infarction, design and implementation of interventions for identifying stressors, as well as their management seem to be crucial.
Reza Ranjbar, Meysam Sarshar, Nour Khoda Sadeghifard,
Volume 20, Issue 81 (9-2012)
Abstract
Background and Objective: Salmonella spp. are enteric pathogens with a worldwide distribution comprising a large number of serovars characterized by different hosts and distribution. Among Salmonella spp., the number of infections and diseases caused by the serotype Salmonella enterica serovar Infantis started to increase significantly in the last decade. The aim of this study was to investigate the genetic diversity of the clinical stains of Salmonella enterica serovar Infantis isolated in Tehran, Iran by using the Ribotyping method. Materials and Methods: In this descriptive study from November 2007 to December 2010, clinical samples, collected from different hospitals in Tehran, were investigated for detection of Salmonella enterica serovar Infantis. Bacterial isolation and identification was achieved through biochemical and bacteriological methods. The Ribotyping technique was applied for the molecular typing of the strain of Salmonella enterica serovar Infantis. Results: Out of the 26 Salmonella serogroup C samples isolated in this study, 19 strains (73%) belonged to Salmonella enterica serotype Infantis. Ribotyping results divided Salmonella enterica serovar Infantis stains into 9 clusters (1c to 9c). The majority (7) of the strains belonged to cluster 1c. Conclusion: The results obtained from the Ribotyping patterns indicate that Salmonella enterica serovar Infantis strains, circulating among the patients in Tehran, belong to a diverse number of clones. Moreover, our data show that Ribotyping is an appropriate method for the molecular typing of Salmonella enterica serovar Infantis strains.
Alireza Sadeghi, Marjaneh Karmi, Maryam Jameshorani, Saeedeh Mazlumzade, Reza Salmani,
Volume 21, Issue 84 (3-2013)
Abstract
Background and Objective: Osteoarthritis is the most common form of arthritis and joint disease and a major cause of disability among middle-aged and elderly people. The process of the progressive destruction of articular cartilage, causes pain and dysfunction of the joints involved. However, the main cause of this disease is not known. In several epidemiological studies from other countries, the problem was reported to be associated with cardiovascular disease. The aim of this study was to find the relationship between carotid artery atherosclerosis in elderly women and osteoarthritis of the joints. Materials and Methods: This cross-sectional study was done on 256 middle-aged and elderly women referred to the Vali-e-Asr Hospital in Zanjan. The patients were divided randomly into two groups of test and control following medical examination. The rate and severity of atherosclerosis and the presence or absence of atherosclerotic plaques were evaluated using Doppler Sono. The data were analyzed using the SPSS software. The mean values were subjected to student t-test and chi-square methods. Results: Of the total of 256 women who participated (mean age 56.79 years) in the study, 30.8% of women with osteoarthritis and 17.5% of the normal groups had carotid artery atherosclerotic plaque (P=0.009 OR=2.1). Even after normalization for age, osteoarthritis was still considered a risk factor for the carotid plaque. The mean carotid artery intima-media thickness was 0.975 mm in the test group compared with 0.910 mm in the control group (P<0.0001). Conclusion: Our findings are consistent with the outcome of some previous studies reporting a significant relationship between osteoarthritis and atherosclerotic disease. A proper drug treatment to reduce the severity of atherosclerosis in order to prevent the progress of osteoarthritis is recommended.
Farzaneh Sadeghi, Mohammad Khalaj-Kondori, Mohammad Ali Hosseinpour Feizi, Farzam Shaikhzadeh Hesari,
Volume 22, Issue 95 (8-2014)
Abstract
Background and Objective: Learning is the acquisition of information about the world and memory is a mechanism to encode, store and retrieve the learned information. Weak memory and learning disorders are the most common cognitive problems. In the present study, the pharmacological effects of aqueous extract of Boswellia on learning and spatial memory in male rats was investigated using the Morris water maze (MWM) test. Materials and Methods: In this experimental study, 21 male rats were divided into 3 groups including a control group (distilled water) and two groups treated with aqueous extract of Boswellia (50 and 100 mg/kg) that received the treatment for 4 weeks. To evaluate learning ability of animals, Morris Water Maze was used. Results: In the first and the last day of training, all groups showed significant reduction in escape latency (P<0.0001) and traveled distance (P<0.0001). In the sixth day of training, both treatment groups showed significant reduction in escape latency (P<0.05) and traveled distance (P<0.05) in comparison with the control group. Conclusion: The results suggest that intake of Boswellia facilitates the learning and spatial memory formation in rats via Morris water maze test method. References 1- Sharifzadeh M, Sharifzadeh K, Naghdi N, Ghahremani MH, Roghani A. Posttraining intrahippocampal infusion of a protein kinase AII inhibitor impairs spatial memory retention in rats. J Neurosci Res. 2005 79(3): 392-400 2- Francis PT, Palmer AM, Snape M, Wilcock GK. The cholinergic hypothesis of Alzheimer’s disease: a review of progress. J Neurol Neurosurg Psychiatry. 1999 66(2): 137-47. 3- Abdel-Tawab M WO, Schubert-Zsilavecz M. Boswellia serrata: an overall assessment of in vitro, preclinical, pharmacokinetic and clinical data. Clin Pharmacokinet. 2011 50(6): 349-69. 4- Hussain H, Al-Harrasi A, Al-Rawahi A, Hussain J. Chemistry and biology of essential oils of genus boswellia. Evid Based Complement Alternat Med. 2013:140509. doi: 10.1155/ 2013/ 140509. 5- Moussaieff A, Mechoulam R. Boswellia resin: from religious ceremonies to medical uses a review of in‐vitro, in‐vivo and clinical trials. J Pharm Pharmacol. 2009 61(10): 1281-93. 6- Thulin M, Warfa A. The frankincense trees (Boswellia spp., Burseraceae) of northern Somalia and southern Arabia. Kew Bulletin. 1987: 487-500. 7- Sharma A, Mann A, Gajbhiye V, Kharya M. Phytochemical profile of Boswellia serrata: An overview. Pharmacog Rev. 2007 1(1): 137. 8- Poeckel D, Werz O. Boswellic acids: biological actions and molecular targets. Curr Med Chem. 2006 13(28): 3359-69. 9- Kimmatkar N, Thawani V, Hingorani L, Khiyani R. Efficacy and tolerability of Boswellia serrata extract in treatment of osteoarthritis of knee–A randomized double blind placebo controlled trial. Phytomedicine. 2003 10(1): 3-7. 10- Marshall S. Frankincense: festive pharmacognosy. Pharmaceutical J. 2003 271 (7280): 862-4. 11- Hosseini -Sharifabad M, Esfandiari E, Alaei H. Effects of frankincense aqueous extract during gestational period on increasing power of learning and memory in adult offsprings. Journal of Isfahan Medical School (iums). 2004 21(71): 16-20. 12- Hosseini-sharifabad M, Esfandiari E. Alaee, H. Moatar F. Effect of maternal consumption of aqueous extract of the gum resin of Boswellia serrata during lactation on increasing power of learning and memory in adult off springs. Iran J Basic Med Sci. 2003 6(3): 207-11. 13- Alaei H, Motahar F, Tory L. Effects of the abstract of oliban on learning and memory. J Ghazvin Univ Med Sci. 1999 21: 21-8. 14- Mahmoudi A, Hosseini-Sharifabad A, Monsef-Esfahani HR,et al. Evaluation of systemic administration of Boswellia papyrifera extracts on spatial memory retention in male rats. J Nat Med. 2011 65(3-4): 519-25. 15- Fathi FHH, Ali Hemmati AR, Banan Khojasteh SM. Effects of sesame oil on improving spatial memory in alzheimer's disease. J Babol Univ Med Sci. 2014 16(2): 34-41. 16- Hosseini M, Hadjzadeh MA, Derakhshan M, Havakhah S, Rassouli FB, Rakhshandeh H, Saffarzadeh F. The beneficial effects of olibanum on memory deficit induced by hypothyroidism in adult rats tested in Morris water maze. Arch Pharm Res. 2010 33(3): 463-8. 17- Hosseini M, Shafei MN, Safari V, Taiarani Z, Kafami Ladani M, Sadeghian R. The effects of olibanum administered to methimazole-treated dams during lactation on learning and memory of offspring rats. Nat Prod Res. 2012 26(16): 1544-8. 18- Hosseini-Sharifabad M, Esfandiari E. Effect of Boswellia serrata gum resin on the morphology of hippocampal CA1 pyramidal cells in aged rat. Anat Sci Int. 2014 Feb 11. [Epub ahead of print] 19- Jalili C, Salahshoor MR, Moradi S, Pourmotabbed A, Motaghi M. The therapeutic effect of the aqueous extract of boswellia serrata on the learning deficit in kindled rats. Int J Prev Med. 2014 5(5): 563-8. 20- Karima O, Riazi G, Yousefi R, Movahedi AAM. The enhancement effect of beta-boswellic acid on hippocampal neurites outgrowth and branching (an in vitro study). Neurol Sci. 2010 31(3): 315-20. 21- Lee S-C, Moon Y-S, You K-H. Effects of red ginseng saponins and nootropic drugs on impaired acquisition of ethanol-treated rats in passive avoidance performance. J Ethnopharmacol. 2000 69(1): 1-8. 22- Savelev S, Okello E, Perry N, Wilkins R, Perry E. Synergistic and antagonistic interactions of anticholinesterase terpenoids in Salvia lavandulaefolia essential oil. Pharmacol Biochem Behav. 2003 75(3): 661-8. 23- Singh G, Atal C. Pharmacology of an extract of salai guggal ex-Boswellia serrata, a new non-steroidal anti-inflammatory agent. Agents Actions. 1986 18(3-4): 407-12. 24- Dhingra D, Parle M, Kulkarni S. Memory enhancing activity of Glycyrrhiza glabra in mice. J Ethnopharmacol. 2004 91(2): 361-5. 25- Mayford M, Kandel ER. Genetic approaches to memory storage. Trends Genet. 1999 15(11): 463-70. 26- Tully T, Bourtchouladze R, Scott R, Tallman J. Targeting the CREB pathway for memory enhancers. Nat Rev Drug Discov. 2003 2(4): 267-77.
Hamidreza Sadeghipour, Farhad Daryanoosh, Mohsen Salesi,
Volume 23, Issue 96 (3-2015)
Abstract
Background and Objective: Chemerin and vaspin are adipose tissue-driven adipokines and appear to be associated with inflammation and insulin resistance. The aim of this study was to investigate the effect of 12 weeks of aerobic interval training on chemerin, vaspin and insulin resistance index in overweight students. Materials and Methods: Twenty four male students voluntarily participated in this study and were randomly divided into experimental and control groups (n=12). The experimental group took part in an aerobic interval training program (3 days/wk, continued for 12 weeks, 40 minutes each session) and the control group continued their routine daily life. Before and after this intervention program, chemerin,vaspin and insulin resistance index were measured. Paired and independent t- tests and Pearson’s correlations were used for data analysis. Results: Results showed a significant decrease in chemerin concentration (t= 5.02, P= 0.001) and insulin resistance index (t= 3.73, P= 0.003) in the experimental group after 12 weeks of aerobic interval training. The results did not show any significant change in the serum vaspin (t= 2.04, P= 0.06). Also, there was a significant relationship between insulin resistance index and both chemerin (P= 0.007) and vaspin levels (P= 0.04). Conclusion: In conclusion, aerobic interval training affects serum chemerin and insulin resistance and suggests that it can play a major role in reducing inflammatory responses caused by overweight and obesity.
Homeir Zardooz, Nahid Sarahian, Hedayat Sahraei, Bahareh Sadeghi,
Volume 23, Issue 101 (8-2015)
Abstract
Background and Objective: Memantine is a glutamate N-Methyl-D-Aspartate (NMDA) receptor antagonist with low receptor binding affinity which is currently used in moderate to severe Alzheimer’s disease. So far, the effect of memantine on stress system has not been investigated. This study focused on the effects of both peripheral and intra-accumbal memantine administration on plasma corticosterone concentration in female NMRI mice.
Materials and Methods: Four groups of mice (n=6/group) received different doses of memantine (1, 0.5 and 0.1 mg/kg) intraperitoneally. In this groups were canulated bi- or unilaterally in the nucleus accumbens by stereotaxic instrument. After a week of recovery, memantine (1, 0.5 and 0.1 µg/kg mouse) was administrated to the animals intra-accumbally. Blood samples were taken from retro-orbital sinus of all animals 30 min (intraperitoneally administration) or 5 min (intra-accumbally) following memantine injection for the evaluation of plasma corticosterone.
Results: Intraperitoneal memantine injection led to a dramatic increase in plasma corticosterone concentration in a dose-independent manner. On the other hand, intra-accumbal memantine administrations increased plasma corticosterone level significantly (P<0.001) in a dose and site –dependent manner.
Conclusion: Memantine may have a severe effect on stress system by virtue of plasma corticosterone level increment which may be the side effect of this medication.
Masoud Hejazi, Afsaneh Sobhi, Aslan Sadeghi Jahan,
Volume 24, Issue 104 (5-2016)
Abstract
Background and Objective: Pervasive prevalence of mental disorders among drug dependent individuals may reduce the success of treatment for drug addiction. In this study, the effect of life skills training on attenuation of anxiety and depression among drug dependent people referred to drop-in centers for harm reduction in Zanjan City was investigated.
Materials and Methods: The population included 90 drug dependent subjects referred to drop-in center for harm reduction in Zanjan City. Using convenience sampling method, the researchers took advantage of pretest-posttest control group design throughout this study. Beck Anxiety Inventory and Beck Depression Inventory were implemented as instruments. Teaching life skills to the experimental group was carried out for 8 sessions 90 minutes each. Analysis of covariance in inferential statistics was used to analyze the collected data.
Results: The results exhibited diminution in the experimental group’s post-test anxiety and depression scores.
Conclusion: Life skills training can be valuable in reducing depression among drug abusers and in the similar way can be efficient in reducing mental disorders of drug dependent people.
Leyla Bahrami, Bahram Sadeghi Bigham, Kurosh Kamali,
Volume 24, Issue 105 (6-2016)
Abstract
Background and Objective: Endometriosis is a prevalent disease in women which may lead to infertility or low fertility. Grasping the genetic grounds for the disease may contribute to its treatment because it is presumed that genetic factors predispose to endometriosis risk factors.
Materials and Methods: 9 genes involved in endometriosis in patients suffering from endometriosis and also in healthy individuals (total 260 samples) were examined. The data were obtained from Ibn Sina (Jahad Daneshgahi) Research Center of new Technologies in Biological Sciences Institute. The study incorporated standard process Crisp for data mining. Weka data mining and software modeling were implemented with the aid of four algorithms.
Results: Comparison of four algorithms implied prominent accuracy of K-Star model. Meanwhile, filtering, while reducing the percentage of models, presented a positive impact on the MLP model. The lowest percentage pertained to Naïve Bayes.
Conclusion: K-Star model without any filtering proved to have the highest accuracy in the early diagnosis of endometriosis.
Parvin Khodarahmi, Nasim Hayati Roodbari, Kazem Parivar, Marjan Sadeghian,
Volume 24, Issue 107 (8-2016)
Abstract
Background and Objective: Iron oxides nanoparticles have numerous applications in medical industry. Nanoparticles are particles with 100 nm in diameter. Despite their various applications, toxic effects of Iron oxide nanoparticles call for supplementary investigations. In this study, the toxic effect of iron oxide nanoparticles on lung development in fetal mice was examined.
Materials and Methods: Using in vivo technique, the mice were allocated to one of the following groups: the control group without the effect of nano iron oxide, sham group with injection of distilled water and experimental groups with low doses (10, 30 and 50 µg /kg) and high doses (10, 30 and 30 mg/kg) of nano iron oxide with 20 nm in diameters injected intraperitoneally on day 9 of pregnancy. On day 16 of pregnancy, embryos were removed and the lung tissue sections were assessed.
Results: The findings showed a significant decrease in the length of the fetus with 30 and 50 mg/kg doses and a significant decrease in the fetal weight and number of primitive bronchioles with 10, 30 and 50 mg/kg. Results also showed a significant increase in lung weight and lung length with doses of 30 and 50 µg/kg, a significant increase in the number of alveolus with doses of 10, 30, 50 µg/kg and 10 mg/kg and a significant increase in the number of necrotic cells with doses of 30 and 50 mg/kg.
Conclusion: Iron oxide nanoparticles showed dual effects. It is asserted throughout this study that fetal lung development increases at low doses and necrotic effect increases with high doses.
Abbas Ghotaslou, Hasan Boustani, Esmaeil Rostami, Omid Ghalesardi, Mohammad Hadi Sadeghian,
Volume 25, Issue 112 (7-2017)
Abstract
Background and Objective: Acute lymphoblastic leukemia is a disorder caused by the proliferation of malignant immature lymphoid cells. GRAF (GTPase regulator associated with focal adhesion kinase) which acts as a tumor suppressor gene is disabled in hematologic malignancies due to genetic and epigenetic abnormalities. In this study, GRAF gene expression was inspected in patients with B-ALL.
Materials and Methods: Peripheral blood samples from 60 patients with B-ALL and 30 healthy controls were collected. DNA was extracted and cDNA was synthesized. GRAF gene expression was measured using Real-Time PCR and the relationship between GRAF expression and clinical and laboratory findings were investigated.
Results: GRAF gene expression was significantly decreased in patients compared to the control group (0.87).
The number of patients who had a reduction in their GRAF gene expression was 73.4% (44 of 60 patients). GRAF expression had no significant difference in the FAB morphological subtypes.
Conclusion: Results of this study showed that GRAF gene expression is decreased in acute lymphoblastic leukemia. The reduction in GRAF gene expression confirms its tumor suppressive role in this leukemia.
Alireza Sadeghi, Maryam Jameshorani, Shima Akbari, Saeiideh Mazloomzadeh, Ayoub Pezeshki,
Volume 25, Issue 113 (8-2017)
Abstract
Background and Objective: Behcet's syndrome is a chronic vasculitis disease that affects multiple organs, including the eyes. Since the involvement of eyes due to inflammation can lead to blindness, therefore it is important that the identifying factors that are associated with increased inflammation be recognized. In recent years the potential role of mineral elements such as zinc in inflammatory conditions has been suggested. The aim of this study was to evaluate the level of zinc in Behcet's disease with eye involvement.
Materials and Methods: This cross-sectional study was carried out on 75 patients with Behcet's disease who were divided into three groups consisting of active uveitis, without uveitis and improved uveitis. Venous blood samples were collected from all patients to measure zinc levels using ELISA. Data were analyzed using SPSS software.
Results: The mean serum zinc level was 130 ± 89/44 in patients with active uveitis, 147 ± 92/26 in the group with improved uveitis and 146 ± 92/24 in the control group. In patients with active uveitis serum zinc levels were lower than the other two groups but this difference was not statistically significant.
Conclusion: In this study, there was no significant reduction in serum zinc levels in active uveitis group. Therefore, unlike similar studies, we could not find evidence to claim that Behcet's disease is associated with zinc deficiency and its effects on the exacerbation of ocular inflammation at least in our patients. Therefore, further studies with a larger population of patients and more accurate methods of measurement can be helpful to elucidate such relation.
Omalbanin Mokhlesi, Mohammad Hadi Sadeghian, Arezoo Shajiei, Maryam Sheikhi, Payam Siyadat, Mohammad Mehdi Kooshyar, Hossein Rahimi, Nafiseh Amini, Maliheh Dadgar Moghadam, Hossein Ayatollahi, Seyyede Fatemeh Shams, Zahra Khoshnegah,
Volume 31, Issue 146 (May & June 2023)
Abstract
Background and Objective: Chronic myeloid leukemia (CML) is a myeloproliferative disorder caused by an aberrant BCR-ABL fusion protein. Imatinib mesylate (IM) is a tyrosine kinase inhibitor that induces clinical remissions in chronic-phase CML patients. The T315I mutation at the gatekeeper residues of BCR-ABL confers resistance to both IM and second-generation TKIs, including dasatinib and nilotinib. Our objective was to determine the prevalence of T315I mutation between two groups of CML patients before and during Imatinib treatment in North-East of Iran.
Materials and Methods: This study was conducted on 100 newly diagnosed cases of CML (before commencing IM treatment) and 25 IM-resistant CML patients. PCR-RFLP, ASO-PCR, and direct sequencing were performed to detect T315I mutations.
Results: The median age of newly-diagnosed and IM-resistant patients was 48±14 and 50±12.3 years, respectively. Males/Females ratio was 1 and 1.08 for newly diagnosed and IM-resistant patients, respectively. There was no significant difference regarding the age and sex between the two groups. During the study, T315I mutational analysis was performed for all 125 patients. The prevalence of T315I mutation was 0% and 4% for newly-diagnosed and IM-resistant patients, respectively. T315I mutation was not detected before IM administration, although it was detected in 1 (4%) among resistant patients who were at least 6-months on IM treatment.
Conclusion: These observations suggest that T315I mutation may be categorized as secondary resistance and induce clonal expansion due to BCR/ABL instability. Hence, BCR-ABL mutations are less likely to appear before the onset of treatment, as presented in our study.
Alireza Sadeghi, Mina Rostami, Nazila Nasiri, Robabeh Hatami, Arezoo Karimi Moghaddam, Zhaleh Karimi Moghaddam, Alireza Zeraatchi,
Volume 31, Issue 149 (November & December 2023)
Abstract
Background and Objective: Rheumatoid Arthritis (RA) is an autoimmune-driven chronic systemic inflammatory disease. It could result in miscarriage, pre-eclampsia, and preterm labor, among other unfavorable pregnancy outcomes. Thus, this study was conducted to investigate the adverse pregnancy outcomes in pregnant women with RA.
Materials and Methods: Two cohorts of pregnant women with and without RA referred to the Ayatollah Mousavi Hospital's Gynecology and Obstetrics Clinic in Zanjan, Iran, during 2019-2020 were enrolled in a retrospective matched cohort study. Using their medical records, each participant completed a checklist of study variables. We used binary Logistic regression, chi-square test, Analysis of Variance, and independent samples t-test to analyze data using SPSS v.23.
Results: The study included 280 pregnant women. The mean age of the RA and control group (pregnant women without RA) was 32.4 ± 6.6 and 29.5 ± 6.7 years, respectively. The most prevalent adverse outcome was spontaneous abortion (54, 19.28%), which was significantly higher in the RA group (25% vs. 13.6%, P= 0.015). Cesarean section (24.3% vs. 10.7%, P= 0.003) and low birth weight (LBW) were both significantly higher in the RA group (15% vs. 5%, P= 0.005). RA increased the probability of spontaneous abortion, cesarean section, and LBW by more than 1.3 (odds ratio, 1.38; 95% CI, 0.45-5.46; P= 0.017), 2.2 (odds ratio, 2.24; 95% CI, 1.29-6.54; P =0.004), and 2.6 (odds ratio, 2.67; 95% CI, 1.86-7.05; P= 0.008) times, respectively.
Conclusion: Pregnant women with RA are more likely to experience spontaneous abortion, cesarean section, and having LBW infants comparing to pregnant women without RA.
Niloufar Abdollahi, Homeira Hatami, Dariush Shanehbandi, Mohammad Shabani, Reihaneh Sadeghian,
Volume 32, Issue 150 (January & February 2024)
Abstract
Background & Objective: Toll-like receptors (TLRs) are proteins that play key roles in inflammation. METH and buprenorphine (BUP) both modulate pain, but the exact mechanism underlying their antinociceptive effects is unknown. As a result, the expression of TLR1 and TLR2 genes was examined in METH rats that had been treated with or without BUP.
Materials & Methods: A total of 77 rats were classified into 11 subtypes (n = 7): control (saline), BUP 6 or 10 mg/kg, METH (10 mg/kg), METH+BUP 6 or 10 mg/kg, or withdrawal groups. The treatments were intraperitoneally administered for 5 or 14 days. RT-PCR evaluated genes expressed in the brain stem area.
Results: The results showed that TLR1 gene expression in the METH group (10 mg/kg; 5 days) considerably improved compared with the control group. Furthermore, BUP injection (10 mg/kg) acutely decreased TLR2 gene expression compared with the METH group. In the METH + BUP (10 mg/kg; 14 days) group, TLR1 expression was higher than in the METH group. The coadministration of METH+BUP (10 mg/kg) acutely decreased TLR2 gene expression compared with METH.
Conclusion: There are limited changes in these genes, and their role in METH consumption and inflammation is unclear. Due to the presence of these two genes in the inflammatory pain and addiction signaling pathways, they may have more clear roles in other parts of the nervous system.