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Volume 11, Issue 42 (Mar 2003)
J Adv Med Biomed Res 2003, 11(42): 15-25 |
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Chahardouli B, Mortazavi Y, Ghaffari S H, Alimoghaddam K, Ghavamzadeh A, Bahar B, et al . Kinetics Of Chimerism After Bone Marrow Transplantation By Multiplex Pcr Amplification Of Short Tandem Repeat (Str) Gene Loci. J Adv Med Biomed Res 2003; 11 (42) :15-25
URL: http://journal.zums.ac.ir/article-1-25-en.html
URL: http://journal.zums.ac.ir/article-1-25-en.html
Bahram Chahardouli * 
, Yousef Mortazavi , Seyed Hamidollah Ghaffari , Kamran Alimoghaddam , Ardeshir Ghavamzadeh , Babak Bahar , Masoud Iravani , Seyed Asadollah Mousavi
, Yousef Mortazavi , Seyed Hamidollah Ghaffari , Kamran Alimoghaddam , Ardeshir Ghavamzadeh , Babak Bahar , Masoud Iravani , Seyed Asadollah Mousavi
Abstract: (158779 Views)
Background: Bone marrow transplantation (BMT) has an increasing application in most hematological disorders that cannot be controlled by current chemotherapy strategies. Therefore, the ability to the evaluation of the contribution of donor cells in recipients' blood circulation (chimerism) is important in the kinetics of bone marrow engraftment. The present study was carried out on candidates of BMT at Tehran Shariati hospital in order to evaluate post-transplantation kinetics of chimerism by multiplex polymerase chain reaction.
Materials and Methods: This descriptive study was performed on 360 blood samples from ten patients who underwent blood or bone marrow stem cell transplantation. Six of the patients were suffering from different types of leukemia and four patients had non-malignant hematological disorders. The chimeric status of transplantation was investigated by close molecular monitoring up to six months post-transplantation. Donor and recipient allelic patterns were determined by multiplex PCR using a panel of STR gene markers such as ARA, D4S2366, D16S539, D7S820, D13S317, F13A1, FES/FPS, VWA, CSF1PO, TPOX and TH01 on DNA of blood specimens.
Result: Based on different gene loci a sensitivity of 1-2% was obtained using PCR method. Mixed chimerism was detected in all the patients between days 1 to 9 post-transplantation. Eight patients changed to a complete allelic pattern during the days 9 to 14 and had an uncomplicated post-transplant disease course. Two patients including one with a low-intensity conditioning regimen consistently retained mixed chimerism for up to six months. Most of the patients achieved full chimerism between days nine to fourteen. Hematological and platelet engraftment occurred between days 8-9 and 9-25 post-transplantation respectively.
Conclusion: The data show that STR analysis using multiplex PCR can provide an accurate, rapid, and quantitative assessment of chimerism situations in post-transplantation patients. Such information may be useful for guiding the early implementation of additional treatment designed to circumvent graft failure or suppression of relapse in the future.
Materials and Methods: This descriptive study was performed on 360 blood samples from ten patients who underwent blood or bone marrow stem cell transplantation. Six of the patients were suffering from different types of leukemia and four patients had non-malignant hematological disorders. The chimeric status of transplantation was investigated by close molecular monitoring up to six months post-transplantation. Donor and recipient allelic patterns were determined by multiplex PCR using a panel of STR gene markers such as ARA, D4S2366, D16S539, D7S820, D13S317, F13A1, FES/FPS, VWA, CSF1PO, TPOX and TH01 on DNA of blood specimens.
Result: Based on different gene loci a sensitivity of 1-2% was obtained using PCR method. Mixed chimerism was detected in all the patients between days 1 to 9 post-transplantation. Eight patients changed to a complete allelic pattern during the days 9 to 14 and had an uncomplicated post-transplant disease course. Two patients including one with a low-intensity conditioning regimen consistently retained mixed chimerism for up to six months. Most of the patients achieved full chimerism between days nine to fourteen. Hematological and platelet engraftment occurred between days 8-9 and 9-25 post-transplantation respectively.
Conclusion: The data show that STR analysis using multiplex PCR can provide an accurate, rapid, and quantitative assessment of chimerism situations in post-transplantation patients. Such information may be useful for guiding the early implementation of additional treatment designed to circumvent graft failure or suppression of relapse in the future.
Keywords: SHORT TANDEM REPEAT (STR), ALLOGENEIC BONE MARROW TRANSPLANTATION, CHIMERISM, MULTIPLEX POLYMERASE CHAIN REACTION
Type of Study: Original Research Article |
Received: 2006/09/6 | Accepted: 2017/06/18 | Published: 2017/06/18
Received: 2006/09/6 | Accepted: 2017/06/18 | Published: 2017/06/18
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